Assessment of the Bone Healing Process Mediated by Periosteum-Derived Mesenchymal Stem Cells’ Secretome and a Xenogenic Bioceramic—An In Vivo Study in the Rabbit Critical Size Calvarial Defect Model
Abstract
:1. Introduction
2. Materials and Methods
2.1. Animals
2.2. Periosteum MSC Isolation and Characterization
2.3. Preparation of Secretomes
2.4. Sample Preparation for Proteomic Analysis
2.5. LC–MS-Based Protein Identification
2.6. In Vivo Evaluation of Secretomes’ Biological Response on the Bone Healing Process
2.7. Characterization of the Bone Healing Process
2.7.1. Microtomographic Evaluation
2.7.2. Histological and Histomorphometric Evaluation
2.8. Statistical Analysis
3. Results and Discussion
3.1. Secretome Characterization
3.2. The Bone Healing in Rabbit Calvarial Critical Size Defects
3.3. Secretome Modulation of the Bone Healing Process
Supplementary Materials
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Conflicts of Interest
References
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Osteogenesis-Related Proteins Identified on the Periosteum-Derived MSCs Secretomes | ||
---|---|---|
Common to UndifSec and OsteoSec | Specific to UndifSec | Specific to OsteoSec |
Actinin alpha 4 Annexin A2 Collagen alpha-2(I) chain Peroxiredoxin-1 Collagen alpha-1(XII) chain Chitinase-3-like protein 1 Fibrillin-1 Macrophage colony-stimulating factor 1 receptor Pro-low-density lipoprotein receptor-related protein 1 | Fibrillin-2 Cathepsin K | Collagen alpha-1(I) chain AE binding protein 1 Stanniocalcin-1 |
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Pranskunas, M.; Šimoliūnas, E.; Alksne, M.; Martin, V.; Gomes, P.S.; Puisys, A.; Kaupinis, A.; Juodzbalys, G. Assessment of the Bone Healing Process Mediated by Periosteum-Derived Mesenchymal Stem Cells’ Secretome and a Xenogenic Bioceramic—An In Vivo Study in the Rabbit Critical Size Calvarial Defect Model. Materials 2021, 14, 3512. https://doi.org/10.3390/ma14133512
Pranskunas M, Šimoliūnas E, Alksne M, Martin V, Gomes PS, Puisys A, Kaupinis A, Juodzbalys G. Assessment of the Bone Healing Process Mediated by Periosteum-Derived Mesenchymal Stem Cells’ Secretome and a Xenogenic Bioceramic—An In Vivo Study in the Rabbit Critical Size Calvarial Defect Model. Materials. 2021; 14(13):3512. https://doi.org/10.3390/ma14133512
Chicago/Turabian StylePranskunas, Mindaugas, Egidijus Šimoliūnas, Milda Alksne, Victor Martin, Pedro Sousa Gomes, Algirdas Puisys, Algirdas Kaupinis, and Gintaras Juodzbalys. 2021. "Assessment of the Bone Healing Process Mediated by Periosteum-Derived Mesenchymal Stem Cells’ Secretome and a Xenogenic Bioceramic—An In Vivo Study in the Rabbit Critical Size Calvarial Defect Model" Materials 14, no. 13: 3512. https://doi.org/10.3390/ma14133512