Reducing Efficiency of Fucoxanthin in Diatom Mediated Biofabrication of Gold Nanoparticles
Abstract
:1. Introduction
2. Materials and Methods
2.1. Extraction of Fucoxanthin
2.2. Biofabrication of GNP
2.3. Characterizations of GNPs
2.4. Microscopic Examination of GNP Loaded N. shiloi
2.5. Quantification of Chlorophyll, Carotenoids and Fucoxanthin
3. Results
3.1. Diatom Assisted Biogenesis of GNP
3.2. Morphological Changes in Gold Exposed N. shiloi
3.3. Confirmation of Au3+ Reduction by Isolated Fucoxanthin
3.4. Characterization of Biogenic GNPs
3.5. Variation in Chlorophyll, Carotenoids and Fucoxanthin Content in GNP Loaded N. shiloi
4. Discussion
5. Conclusions
Author Contributions
Funding
Acknowledgments
Conflicts of Interest
References
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Reducing Agents | Concentration of Au3+ Solution (mg L−1) | pH | Reaction Time | Maximum Absorbance (nm) |
---|---|---|---|---|
Whole biomass of N. shiloi | 5 | 4 | 20 days | ~550 |
7 | 25 days | ~550 | ||
8 | 22 days | ~550 | ||
15 | 4 | 7 days | ~536 | |
7 | 15 days | ~550 | ||
8 | 10 days | ~540 | ||
25 | 4 | 3 days | ~530 and ~966 | |
7 | 7 days | ~535 | ||
8 | 5 days | ~539 | ||
Extracted fucoxanthin from N. shiloi | 25 | 6 | 12 h | ~535 |
Standard fucoxanthin | 25 | 6 | 12 h | ~537 |
Parameters | Experimental Condition | Time of Exposure | ||||
---|---|---|---|---|---|---|
30 min | 1 h | 3 h | 12 h | 24 h | ||
Chlorophyll (µg mL−1) | Control | 7.333 ± 0.202 | 7.543 ± 0.223 | 8.066 ± 0.132 | 8.01 ± 0.223 | 8.48 ± 0.245 |
25 mg L−1 Au3+ treated | 17.759 ± 0.329 | 13.354 ± 0.057 | 8.682 ± 0.045 | 7.554 ± 0.040 | 3.847 ± 0.083 | |
Carotenoids (µg mL−1) | Control | 14.296 ± 0.169 | 14.396 ± 0.078 | 14.55 ± 0.101 | 14.626 ± 0.172 | 14.643 ± 0.189 |
25 mg L−1 Au3+ treated | 14.762 ± 0.048 | 27.683 ± 0.386 | 15.66 ± 0.069 | 9.305 ± 0.071 | 5.194 ± 0.042 | |
Fucoxanthin (µg mL−1) | Control | 12.628 ± 0.022 | 12.680 ± 0.038 | 12.748 ± 0.104 | 12.767 ± 0.123 | 12.771 ± 0.126 |
25 mg L−1 Au3+ treated | 12.94 ± 0.039 | 24.099 ± 0.430 | 13.878 ± 0.040 | 8.175 ± 0.043 | 4.481 ± 0.014 |
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Roychoudhury, P.; Dąbek, P.; Gloc, M.; Golubeva, A.; Dobrucka, R.; Kurzydłowski, K.; Witkowski, A. Reducing Efficiency of Fucoxanthin in Diatom Mediated Biofabrication of Gold Nanoparticles. Materials 2021, 14, 4094. https://doi.org/10.3390/ma14154094
Roychoudhury P, Dąbek P, Gloc M, Golubeva A, Dobrucka R, Kurzydłowski K, Witkowski A. Reducing Efficiency of Fucoxanthin in Diatom Mediated Biofabrication of Gold Nanoparticles. Materials. 2021; 14(15):4094. https://doi.org/10.3390/ma14154094
Chicago/Turabian StyleRoychoudhury, Piya, Przemysław Dąbek, Michał Gloc, Aleksandra Golubeva, Renata Dobrucka, Krzysztof Kurzydłowski, and Andrzej Witkowski. 2021. "Reducing Efficiency of Fucoxanthin in Diatom Mediated Biofabrication of Gold Nanoparticles" Materials 14, no. 15: 4094. https://doi.org/10.3390/ma14154094