Preparation and Characterization of Porous Poly(Lactic Acid)/Poly(Butylene Adipate-Co-Terephthalate) (PLA/PBAT) Scaffold with Polydopamine-Assisted Biomineralization for Bone Regeneration
Abstract
:1. Introduction
2. Materials and Methods
2.1. Materials
2.2. Fabrication of Porous PLA/PBAT Scaffold
2.3. Biomimetic Mineralization
2.4. Specimen Characterization
2.4.1. Scaffold Morphology
2.4.2. Physicochemical Characterization
2.4.3. Mechanical Properties
2.4.4. Surface Contact Angle Measurements
2.4.5. Porosity
2.4.6. Water Uptake
2.4.7. In Vitro Degradation Test
2.5. Biological Evaluation
2.5.1. Cell Viability and Biocompatibility
2.5.2. Cell–Scaffold Attachment
2.5.3. Osteogenic Differentiative Property
2.6. Statistical Analysis
3. Results
3.1. Scaffold Morphology
3.2. Physicochemical Characterization of the Scaffolds
Compressive Strength
3.3. Chemical Composition Analysis
3.3.1. FTIR Analysis
3.3.2. XRD Analysis
3.4. Biological Evaluations
3.4.1. Cell Viability and Cell Attachment
3.4.2. The Osteogenesis Property of the Scaffold
4. Discussion
4.1. Scaffold Morphology
4.2. Physicochemical Properties
4.2.1. Compressive Strength
4.2.2. In Vitro Degradation
4.2.3. Chemical Composition
4.3. Biological Properties
5. Conclusions
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Acknowledgments
Conflicts of Interest
References
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Reagent | Order | Amount (g) | Concentration (mM) |
---|---|---|---|
NaCl | 1 | 58.443 | 1000 |
KCl | 2 | 0.373 | 5 |
CaCl2·2H2O | 3 | 3.675 | 25 |
MgCl2·6H2O | 4 | 1.016 | 5 |
NaH2PO4 | 5 | 0.25 | 3.62 |
NaHCO3 | 6 | 0.84 | 10 |
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Suttiat, K.; Wattanutchariya, W.; Manaspon, C. Preparation and Characterization of Porous Poly(Lactic Acid)/Poly(Butylene Adipate-Co-Terephthalate) (PLA/PBAT) Scaffold with Polydopamine-Assisted Biomineralization for Bone Regeneration. Materials 2022, 15, 7756. https://doi.org/10.3390/ma15217756
Suttiat K, Wattanutchariya W, Manaspon C. Preparation and Characterization of Porous Poly(Lactic Acid)/Poly(Butylene Adipate-Co-Terephthalate) (PLA/PBAT) Scaffold with Polydopamine-Assisted Biomineralization for Bone Regeneration. Materials. 2022; 15(21):7756. https://doi.org/10.3390/ma15217756
Chicago/Turabian StyleSuttiat, Kullapop, Wassanai Wattanutchariya, and Chawan Manaspon. 2022. "Preparation and Characterization of Porous Poly(Lactic Acid)/Poly(Butylene Adipate-Co-Terephthalate) (PLA/PBAT) Scaffold with Polydopamine-Assisted Biomineralization for Bone Regeneration" Materials 15, no. 21: 7756. https://doi.org/10.3390/ma15217756