Multiscale Femoral Neck Imaging and Multimodal Trabeculae Quality Characterization in an Osteoporotic Bone Sample
Abstract
:1. Introduction
2. Materials and Methods
2.1. Sample Collection and Preparation
2.2. Sample Extraction and Preparation for Microindentation Test
2.3. X-ray Microtomography Measurements
2.3.1. Image Post-Processing and Analysis
2.3.2. SRµCT (Voxel Size: 0.9 µm)
2.4. Microindentation
2.5. Fourier Transform Infrared Spectroscopy
2.6. Statistical Analysis
3. Results
3.1. Osteocytes Lacunae Characteristics
3.2. Trabeculae Mechanical Properties
3.3. ATR-FTIR Results
4. Discussion
5. Conclusions
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Conflicts of Interest
Appendix A. Femoral Neck Macroscale Analysis
Whole Femoral Neck (51.0 µm) | Femoral Neck Core (4.95 µm) | |||||||
---|---|---|---|---|---|---|---|---|
Ct.Th (mm) | Tb.Th (mm) | Tb.Sp (mm) | BVF | Tb.Th (mm) | Tb.Sp (mm) | BVF | Min Tb.Th (mm) | |
Control | 0.74 ± 0.52 | 0.21 ± 0.10 | 1.03 ± 0.51 | 0.158 | 0.13 ± 0.06 | 1.10 ± 0.29 | 0.079 | 0.057 ± 0.003 |
OsteopS | 0.73 ± 0.48 | 0.18 ± 0.11 | 1.11 ± 0.59 | 0.137 | 0.12 ± 0.06 | 1.41 ± 0.47 | 0.053 | 0.052 ± 0.005 |
Diff | −2% | −12% | 8% | −13% | −5% | 28% | −33% | −8% |
Appendix B. Image Processing Effect on OL Characterization
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Gender | Age (y) | Leg Pos | Height (m) | DXA (g/cm2) | |||
---|---|---|---|---|---|---|---|
Total | Neck | Troch. 2 | |||||
Control | Female | 95 | Right | 1.63 | 0.939 | 0.898 | 0.883 |
OsteopS 1 | Female | 96 | Right | 1.65 | 0.480 | 0.423 | 0.419 |
Total Values | OsteopS | Control | Diff | |
Nb of Analyzed Regions | 5 | 5 | - | |
Bone Volume (mm3) | 0.28 | 0.45 | - | |
OL Number | 4030 | 6649 | - | |
OL Volume (mm3) | 0.0016 | 0.0023 | - | |
OL Density (104 mm−3) | 1.44 | 1.49 | −3% | |
Bone Porosity (%) | 0.59 | 0.52 | +13% | |
Mean Values | OsteopS (mean ± SD) | Control (mean ± SD) | Diff | |
OL Volume (µm3) | 358.08 ± 165.00 | 287.10 ± 160.00 | 25% * | |
OL Surface (µm2) | 225.53 ± 13.75 | 195.00 ± 10.11 | 16% * | |
Lacunar Density (104 mm−3) | 1.60± 0.33 | 1.56 ± 0.16 | 3% | |
OL Region of Action (104 µm−3) | 5.7 ± 2.7 | 6.0 ± 4.0 | −5% | |
OL Principal Axes (µm) | a (length) | 12.13 ± 0.46 | 11.18 ± 0.57 | 8% |
b (width) | 6.68 ± 0.32 | 6.19 ± 0.13 | 8% * | |
c (depth) | 4.40 ± 0.13 | 4.09 ± 0.12 | 8% * | |
OL Shape (Ad) | a/b | 1.90 ± 0.11 | 1.89 ± 0.09 | 1% |
b/c | 1.54 ± 0.06 | 1.53 ± 0.06 | 1% | |
a/c | 2.85 ± 0.07 | 2.82 ± 0.17 | 1% | |
a/(b + c) | 1.13 ± 0.05 | 1.12 ± 0.05 | 1% | |
OL Sphericity (Ad) | 0.80 ± 0.01 | 0.79 ± 0.02 | 0% | |
OL Fractal Anisotropy (Ad) | 0.47 ± 0.02 | 0.46 ±0.02 | 1% |
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Soldati, E.; Roseren, F.; Guenoun, D.; Mancini, L.; Catelli, E.; Prati, S.; Sciutto, G.; Vicente, J.; Iotti, S.; Bendahan, D.; et al. Multiscale Femoral Neck Imaging and Multimodal Trabeculae Quality Characterization in an Osteoporotic Bone Sample. Materials 2022, 15, 8048. https://doi.org/10.3390/ma15228048
Soldati E, Roseren F, Guenoun D, Mancini L, Catelli E, Prati S, Sciutto G, Vicente J, Iotti S, Bendahan D, et al. Multiscale Femoral Neck Imaging and Multimodal Trabeculae Quality Characterization in an Osteoporotic Bone Sample. Materials. 2022; 15(22):8048. https://doi.org/10.3390/ma15228048
Chicago/Turabian StyleSoldati, Enrico, Flavy Roseren, Daphne Guenoun, Lucia Mancini, Emilio Catelli, Silvia Prati, Giorgia Sciutto, Jerome Vicente, Stefano Iotti, David Bendahan, and et al. 2022. "Multiscale Femoral Neck Imaging and Multimodal Trabeculae Quality Characterization in an Osteoporotic Bone Sample" Materials 15, no. 22: 8048. https://doi.org/10.3390/ma15228048