Recent Advances in Decellularized Extracellular Matrix-Based Bioinks for 3D Bioprinting in Tissue Engineering
Abstract
:1. Introduction
2. Bioprinting Technology of Decellularized Extracellular Matrix
2.1. Inkjet-Based Bioprinting
2.2. Laser-Assisted Bioprinting
2.3. Stereolithography-Based Bioprinting
2.4. Extrusion-Based Bioprinting
3. Bioactive Molecules in the Decellularized Extracellular Matrix
4. Construction Methods of Decellularized Extracellular Matrix
4.1. Chemical Methods
4.2. Physical Methods
4.3. Biological Methods
5. The Construction and Modification of Decellularized Extracellular Matrix-Based Bioinks
6. The Applications of Decellularized Extracellular Matrix-Based Bioink for Bioprinting in Tissue Engineering
6.1. Hearts
6.2. Cartilage
6.3. Adipose Tissue
6.4. Skeletal Muscle
6.5. Liver
6.6. Skin
6.7. Cornea
6.8. Brain
6.9. Pancreas
6.10. Trachea
6.11. Blood Vessels
6.12. Tendon
7. Current Challenges and Further Perspectives
7.1. Optimizing the Fabrication Procedure of dECM-Derived Bioinks
7.2. Mechanical Properties
7.3. Long-Term Biosafety of dECM-Derived Bioinks after Implantation
7.4. Drug Discovery and Development
8. Conclusions
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Conflicts of Interest
References
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Bioink Composition | Seed Cells | Biological Factors | Source of dECM | Applications | References |
---|---|---|---|---|---|
Decellularized heart tissue | Rat myoblast cells | - | Porcine heart | Heart tissue regeneration | [142] |
Decellularized heart tissue | Cardiac progenitor cells | VEGF | Left ventricle from the complete porcine heart | Hydrogel patch for cardiac repair | [30] |
Decellularized heart tissue | Human cardiac progenitor cells | Vitamin B2 | Heart tissue from a 6-month-old Korean domestic pig | In vitro fabrication of engineered tissue | [143] |
Decellularized heart tissue | Human iPSCs | - | Heart left ventricles from Yorkshire pigs | Fabrication of patient-specific tissue model | [144] |
Decellularized heart tissue | Neonatal rat cardiomyocytes | - | Heart tissues from 6-month-old Korean domestic pigs | Engineered heart tissue | [145] |
Decellularized cartilage tissue | Human inferior turbinate-tissue-derived MSCs | - | Porcine cartilage tissue | Cartilage tissue regeneration | [142] |
Decellularized cartilage tissue and silk fibroin | Rabbit bone-marrow-derived MSCs | - | Porcine articular cartilage | Developing tissue substitutes with irregular shape | [146] |
Decellularized cartilage tissue and silk fibroin | Rabbit bone-marrow-derived MSCs | TGF-β3 | Articular cartilage tissue from female goats | Cartilage regeneration | [147] |
Decellularized menisci and polyurethane and polycaprolactone polymers | Human bone-marrow-derived MSC | - | Porcine lateral and medial menisci | Meniscus regeneration | [148] |
Decellularized menisci and poly(vinyl alcohol) | - | - | Rabbit menisci | Meniscus regeneration | [149] |
Decellularized adipose tissue | Human adipose-derived stem cells | - | Porcine adipose | Adipose tissue regeneration | [142] |
Decellularized adipose tissue and alginate | Human subcutaneous preadipocyte cells | - | Human adipose | Engineering densely packed adipose tissue | [150] |
Decellularized adipose tissue and plasma | Human dermal fibroblasts | - | Porcine adipose | Tissue substitutes with optimal microenvironment | [151] |
Decellularized adipose tissue | Minced parathyroid glands | - | Porcine adipose | Maintaining biological functions of parathyroid glands | [152] |
Decellularized skeletal muscle | Human skeletal muscle cells | - | Porcine tibialis anterior muscles and descending aortas | Volumetric muscle loss treatment | [153] |
Methacrylate decellularized skeletal muscle | C2C12 cells | - | Lower limb muscle from adult Yorkshire porcine | Muscle regeneration | [154] |
Decellularized liver tissue | HepG2 cells and human MSCs | - | Porcine liver tissue | Liver in vitro models for transplantation and drug screening | [155] |
Decellularized liver tissue | Human iPSCs | - | Liver from three-month-old healthy Yorkshire pigs | Fabrication of patient-specific tissue substitutes | [144] |
Decellularized liver tissue and collagen I | HepG2 cells | - | Porcine liver from Yorkshire pigs | Disease mechanism exploration and drug screening | [156] |
Decellularized skin tissue | Human neonatal epidermal keratinocytes, human adipose-derived MSCs | - | Porcine skin tissue | Skin regeneration | [157] |
Decellularized skin tissue | Human dermal fibroblasts | - | Porcine skin tissue | Dermal substitute | [158] |
Decellularized skin tissue | Mouse fibroblasts | - | Native skin tissues from a Korean domestic pig | Establishing a 3D cell printing process | [159] |
Decellularized cornea and collagen I | Human corneal keratocytes | - | Bovine eyeballs | Cornea substitutes | [160] |
Decellularized cornea | - | - | Bovine eyeballs | Artificial corneas | [161] |
Decellularized brain | Glioblastoma cells and endothelial cells | - | Cephalic parts of market pigs | In vitro disease model | [162] |
Decellularized pancreatic tissues | Rat islets and endothelial cells | - | Porcine pancreatic tissue | Fabricating 3D pancreatic tissue constructs | [163] |
Decellularized pancreatic tissues | - | - | Porcine pancreatic tissue | Pancreatic tissue substitutes | [164] |
Decellularized tracheal mucosa | Endothelial cells and fibroblasts | - | Porcine tracheal mucosa | Functional airway-on-a-chip | [165] |
Decellularized trachea | Human inferior turbinate MSCs | - | Porcine trachea | Tracheal reconstruction | [166] |
Decellularized vascular tissue | Endothelial progenitor cells | - | Porcine descending aortas of pigs | Therapy for ischemic disease | [31] |
Decellularized vascular tissue | Endothelial cells | - | Fresh porcine aortic tissue | In vitro vascular models | [167] |
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Zhe, M.; Wu, X.; Yu, P.; Xu, J.; Liu, M.; Yang, G.; Xiang, Z.; Xing, F.; Ritz, U. Recent Advances in Decellularized Extracellular Matrix-Based Bioinks for 3D Bioprinting in Tissue Engineering. Materials 2023, 16, 3197. https://doi.org/10.3390/ma16083197
Zhe M, Wu X, Yu P, Xu J, Liu M, Yang G, Xiang Z, Xing F, Ritz U. Recent Advances in Decellularized Extracellular Matrix-Based Bioinks for 3D Bioprinting in Tissue Engineering. Materials. 2023; 16(8):3197. https://doi.org/10.3390/ma16083197
Chicago/Turabian StyleZhe, Man, Xinyu Wu, Peiyun Yu, Jiawei Xu, Ming Liu, Guang Yang, Zhou Xiang, Fei Xing, and Ulrike Ritz. 2023. "Recent Advances in Decellularized Extracellular Matrix-Based Bioinks for 3D Bioprinting in Tissue Engineering" Materials 16, no. 8: 3197. https://doi.org/10.3390/ma16083197