Identification and Characterization of Nectria pseudotrichia Associated with Camellia Canker Disease in China
Abstract
:1. Introduction
2. Materials and Methods
2.1. Study Site and Field Surveys of Canker Disease in C. japonica
2.2. Fungal Isolation
2.3. Morphological Characterization
2.4. DNA Extraction, Amplification, Sequencing, and Phylogenetic Analysis
2.5. Pathogenicity Tests
3. Results
3.1. Field Surveys of Canker Disease in C. japonica
3.2. Isolation, Morphological Structures and Cultural Characteristics of the Pathogenic Fungus of Camellia Canker
3.3. Phylogenetic Analysis
3.4. Pathogenicity Tests
4. Discussion
Author Contributions
Funding
Data Availability Statement
Conflicts of Interest
References
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Species | Strain 1 | Geographic Origin | GenBank Accession No. | ||
---|---|---|---|---|---|
ITS | LSU | tef1-α | |||
Nectria antarctica | A.R.2767 | USA | HM484556 | HM484560 | HM484516 |
N. aurantiaca | CBS 308.34 | UK | JF832628 | JF832682 | JF832519 |
N. balansae | A.R. 4446; CBS 123351 | France | HM484552 | GQ505996 | HM484525 |
N. balansae | A.R. 4635 | China | JF832653 | JF832711 | JF832522 |
N. berberidicola | A.R. 4662 | France | JF832662 | JF832712 | JF832538 |
N. cinnabarina | A.R. 4477 | France | HM484548 | HM484562 | HM484527 |
N. dematiosa | G.J.S. 94-37 | USA | HM484557 | HM484561 | HM484534 |
N. magnispora | TPP-h122 | Japan | JF832663 | JF832683 | JF832539 |
N. mariae | A.R. 4274 | France | JF832629 | JF832684 | JF832542 |
N. nigrescens | A.R. 4211 | USA | HM484707 | HM484720 | HM484672 |
N. polythalama | A.R. 4586 | New Zealand | JF832638 | JF832695 | JF832523 |
N. pseudocinnabarina | G.J.S. 09-1359 | Venezuela | JF832642 | JF832697 | JF832533 |
N. pseudotrichia | LPNP | China | OK562743 | OK562730 | OL440127 |
N. pseudotrichia | CBS551.84; CBS 451.85 | Japan; Honduras | HM484554 | GQ506000 | HM484532 |
N. pseudotrichia | MAFF 241394 | Japan | JF832639 | JF832705 | JF832527 |
N. pseudotrichia | ICMP 2245 | Japan | JF832640 | JF832701 | JF832532 |
N. pseudotrichia | MAFF 241452 | Papua New Guinea | JF832649 | JF832706 | JF832531 |
N. pseudotrichia | CBS 652.83 | Japan | JF832648 | JF832703 | JF832528 |
N. pseudotrichia | Y.H. 10-04 | Venezuela | JF832650 | JF832704 | JF832529 |
N. pseudotrichia | G.J.S. 09-1329 | USA | JF832647 | JF832702 | JF832530 |
N. pseudotrichia | CFCC 52122 | Venezuela | MG231953 | MG231974 | MG232016 |
N. pseudotrichia | CFCC 52123 | China | MG231954 | MG231975 | MG232017 |
N. pseudotrichia | CFCC 52124 | China | MG231955 | MG231976 | MG232018 |
N. novae-zealandiae | HMAS 252485 | China | KM026503 | KM026504 | KM026506 |
N. ulmicola | CFCC 52117 | China | MG231959 | MG231980 | MG232022 |
N. australiensis | H13 | China | GU075855 | DQ119569 | HM054076 |
N. eustromatica | CBS 121896 | China | HM534896 | HM534896 | HM534875 |
N. sordida | G.J.S. 86-117 | Croatia | HM484857 | HM484868 | HM484848 |
N. balansae | CFCC 52119 | French Guiana | MG231956 | MG231977 | MG232019 |
Cosmospora coccinea | CBS 114050; AR2741 | China | FJ474072 | GQ505990 | AY489629 |
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Geng, X.; Liu, Y.; Li, J.; Li, Z.; Shu, J.; Wu, G. Identification and Characterization of Nectria pseudotrichia Associated with Camellia Canker Disease in China. Forests 2022, 13, 29. https://doi.org/10.3390/f13010029
Geng X, Liu Y, Li J, Li Z, Shu J, Wu G. Identification and Characterization of Nectria pseudotrichia Associated with Camellia Canker Disease in China. Forests. 2022; 13(1):29. https://doi.org/10.3390/f13010029
Chicago/Turabian StyleGeng, Xiansheng, Ying Liu, Jiyuan Li, Zhihong Li, Jinping Shu, and Guiyang Wu. 2022. "Identification and Characterization of Nectria pseudotrichia Associated with Camellia Canker Disease in China" Forests 13, no. 1: 29. https://doi.org/10.3390/f13010029
APA StyleGeng, X., Liu, Y., Li, J., Li, Z., Shu, J., & Wu, G. (2022). Identification and Characterization of Nectria pseudotrichia Associated with Camellia Canker Disease in China. Forests, 13(1), 29. https://doi.org/10.3390/f13010029