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Forests
Volume 13
Issue 12
10.3390/f13122123
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Article
Peer-Review Record

Expression Pattern Analysis of Larch WRKY in Response to Abiotic Stress

Forests 2022, 13(12), 2123; https://doi.org/10.3390/f13122123
by Chen Wang, Qingrong Zhao, Lei Zhang and Hanguo Zhang *
Reviewer 1:
Xueqin Wan
Reviewer 2: Anonymous
Forests 2022, 13(12), 2123; https://doi.org/10.3390/f13122123
Submission received: 14 November 2022 / Revised: 8 December 2022 / Accepted: 9 December 2022 / Published: 11 December 2022
(This article belongs to the Section Genetics and Molecular Biology)

Round 1

Reviewer 1 Report

About the paper, with my knowledge in relation to transcription factors analysis, I think this a complete article because includes both bioinformatics and expression pattern analysis. I have some comments on the manuscript.

1. How the sequence of WRKY transcription factor family come from? Only four WRKY transcription factor are detected in Larix olgensis? I think this information should supplied in the section of materails.

2. Did you only use one reference gene? According to MIQE (Minimum Information for Publication of Quantitative Real-Time PCR): «Unless fully validated single reference genes are used, normalisation should be performed against multiple reference genes, chosen from a sufficient number of candidate reference genes tested from independent pathways using at least one algorithm..» (Bustin, S.A., Beaulieu, JF., Huggett, J. et al. MIQE précis: Practical implementation of minimum standard guidelines for fluorescence-based quantitative real-time PCR experiments. BMC Molecular Biol. 2010. 11, 74. https://doi.org/10.1186/1471-2199-11-74)

3. Subcellular localization analysis would be supplied in the bioinformatics analysis. It would help to enrich the content of function prediction of WRKY TFs.

Best regards.

 

Author Response

Please see the attachment.

Author Response File: Author Response.docx

Reviewer 2 Report


Comments for author File: Comments.docx

Author Response

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Author Response File: Author Response.docx

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