Full-Length Transcriptome Sequencing and Identification of Genes Related to Terpenoid Biosynthesis in Cinnamomum migao H. W. Li
Round 1
Reviewer 1 Report
The study was conducted to report the transcriptome data of C. migao using full-length transcriptome and NGS. This is a good study since the genome data of the plant species is not available. Overall, the authors have reported good results but certain parts of the manuscript need to be improved extensively. Authors also should send the manuscript for English language editing. M&M is too shallow. The author should write all the methods/procedures in detail. Everything should be written out in detail for readers to understand or for them to refer to.
Abstract:
space between C. and migao
Try to avoid using keywords already stated in the title of the manuscript
Line 38: in vitro of should "of" be italicised too?
Line 39: space between C. and migao Check all places too.
Line 55-56: Although some researchers.... Hanging sentence. Please rephrase.
Line 60: However,
Line 72-77: Rephrase and check language for clarity.
Line 87-88: Four sample with three repeats but equal to 21 RNA? Please correct this sentence. Do you mean that you have 7 samples (CMFI, CMFII, CMFIII, CMFIV, roots, stems, leaves)?
Line 89: What parameters were measured using Agilent 2100? How RNA was considered good/bad quality using this analyzer?
Line 91-93: Elaborate more in detail how you synthesis the full-length cDNA.
Line 99: Elaborate more on the sequencing using Illumina platform. Any specific requirement has to be met to sequence the cDNA or what parameters were chosen to run the sequencing.
Line 109-111: What software was used for the NGS transcriptome data? What do you mean by low-quality data? How you filter out? Please write everything in detail.
Line 135-136: sentence not clear. Rephrase.
Line 136: action gene?
Line 139-141: Write in detail how you prepare the RT-qPCR mix and the parameter to run the RT-qPCR..temperature etc.
Comments for author File: 
Comments.pdf
Major English language editing is required. Please send it to a professional English editing service provider.
Author Response
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Author Response File: 
Author Response.docx
Reviewer 2 Report
IN this paper the Cinnamomum migao the genome data of this plant species resource using molecular biological technology meaning full-length transcriptome and NGS sequencing was firstly used to obtain the transcriptome data of C. migao. A total of 73,57524 redundant transcripts from 39.9Gb raw data was obtained and 70,427 transcripts were annotated. Meanwhile, SSR, lncRNA and TFs were predicted using powerful bioinformatic tools. Finally, 57 DETs26 involved in the biosynthesis of terpenoids were identified. This study provide a basis data for the next research in gene mining, genetic breeding and metabolic engineering in C.migao Comments papers isawesome showing good charts and figures related to gene biotechnology for the first time please check English language
please check English language
Author Response
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Author Response File: Author Response.docx
Reviewer 3 Report
Comment 1: Provide justification for the novelty of this study in comparison to the literature cited.
Comment 2: What is the main research question addressed in this study?
Comment 3: What does this study contribute to the subject area in comparison to other published material?
Comment 4: The abstract paragraph is too short it should be more informative and clearer.
Comment 5: Acronyms/Abbreviations should be defined the first time they appear in the abstract, the main text, and the first figure or table (when defined for the first time, the acronym/abbreviation should be added in parentheses).
Comment 6:2.1. Plant materials part is missing the field location (by GPS), field environmental conditions, the soil type, irrigation, and the age of harvesting the plants.
Comment 7: In line 83, four different developmental stages were collected and used for RNA83 extraction, which named CMFI, CMFII, CMFIIII and CMFIV. What is the means of these symbols?
Comment 8: In table 1, the plant name must be in italics (check it throughout the whole manuscript).
Comment 9: Figure 2, the resolution is very bad and it needs correction.
Comment 10: The discussion part is very poor and needs to be more informative. Authors need to compare the results with recent studies to demonstrate the importance of your research.
Comment 11: The same paragraph is in both the conclusion and the abstract. Authors need to present your conclusion separately.
English revision by a Native speaker is recommended
Author Response
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Author Response File: Author Response.docx
Round 2
Reviewer 1 Report
The author has made a good effort in revising the manuscript.
Authors should include their response to the comments on the determination of good/bad quality RNA in the materials and methods section as it is a crucial process for the experiment. Explain clearly in the manuscript.
Other parts are fine and good.
Author Response
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Author Response File: Author Response.docx
Reviewer 3 Report
This manuscript has revised all of my comments. I think that the current version needs English editing by a native speaker to meet the publishing requirements in Forests.
English revision by a native speaker is recommended
Author Response
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Author Response File: Author Response.docx
