Distribution Characteristics and Driving Factors of the Bacterial Community Structure in the Soil Profile of a Discontinuous Permafrost Region
Abstract
:1. Introduction
2. Materials and Methods
2.1. Research Area and Collection of Soil Samples
2.2. Soil Sample Testing
2.3. DNA Extraction, Amplification, and Sequencing
2.4. Sequencing of Data Processing
2.5. Statistical Analysis
3. Results
3.1. Physical and Chemical Properties of Different Soil Layers
3.2. Richness and Diversity of Soil Bacterial Communities along Soil Profiles in Permafrost Regions
3.3. Soil Bacterial Community Structure along Soil Profiles in Permafrost Regions
3.4. Relationship between the Major Bacterial Phyla and Environmental Factors along the Soil Profiles in Permafrost Regions
3.5. Prediction and Analysis of Soil Bacterial Community Function along Soil Profiles in Permafrost Regions
3.6. Soil Profiles Affect Soil Bacterial Metabolic Pathways in Permafrost Regions
4. Discussion
4.1. Differences in Soil Profiles Affect the Composition of the Microbial Community in Permafrost Areas
4.2. Differences in Soil Profiles Affect Bacterial Richness and Diversity in Permafrost Areas
4.3. Effects of the Environment on the Soil Bacterial Community in the Permafrost Area
4.4. Analysis of the Prediction Function of Bacterial Communities along Soil Profiles in Permafrost Regions
5. Conclusions
Supplementary Materials
Author Contributions
Funding
Data Availability Statement
Conflicts of Interest
References
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Sample | pH | Cond (S/m) | ST (°C) | SWC (%) | TOC (g/kg) | TN (g/kg) | TP (g/kg) | HN4+-N (mg/kg) | HN3−-N (mg/kg) |
---|---|---|---|---|---|---|---|---|---|
Upper active layer | 5.41 ± 0.03 a | 5.01 ± 0.24 c | 0.07 ± 0.05 a | 19.57 ± 1.48 b | 33.82 ± 0.61 b | 2.13 ± 0.09 a | 828.33 ± 4.43 b | 12.33 ± 0.19 a | 4.33 ± 0.09 a |
Lower active layer | 5.28 ± 0.01 b | 6.32 ± 0.09 b | 0.30 ± 0.08 a | 22.27 ± 0.71 a | 43.59 ± 1.44 a | 1.79 ± 0.02 b | 1115.50 ± 15.24 a | 10.90 ± 0.28 b | 2.08 ± 0.09 b |
Transition layer | 5.01 ± 0.04 c | 5.71 ± 0.12 b | 0.17 ± 0.34 a | 22.8 ± 0.94 a | 20.31 ± 0.64 c | 1.41 ± 0.04 c | 691.4 ± 22.62 c | 8.14 ± 0.09 d | 1.14 ± 0.08 c |
Permafrost layer | 4.91 ± 0.05 c | 7.85 ± 0.71 a | −1.97 ± 0.48 b | 21.97 ± 0.09 a | 15.99 ± 0.43 d | 1.52 ± 0.02 c | 507.44 ± 11 d | 10.20 ± 0.14 c | 1.33 ± 0.03 c |
Sample | Shannon | Simpson | ACE | Chao1 | Good’s Coverage (%) |
---|---|---|---|---|---|
Upper active layer | 4.81 ± 0.06 a | 0.021 ± 0.002 b | 811.94 ± 44.33 ab | 801.42 ± 31.74 ab | 99.43 ± 0.13 |
Lower active layer | 4.44 ± 0.01 b | 0.037 ± 0.002 ab | 839.00 ± 4.42 a | 856.42 ± 17.47 a | 99.28 ± 0.04 |
Transition layer | 4.20 ± 0.15 b | 0.061 ± 0.02 a | 725.75 ± 13.66 b | 731.45 ± 14.12 b | 99.38 ± 0.03 |
Permafrost layer | 4.45 ± 0.09 b | 0.028 ± 0.005 b | 751.90 ± 11.95 b | 759.87 ± 15.4 b | 99.51 ± 0.14 |
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Liu, Q.; Song, L.; Zou, S.; Wu, X.; Zang, S. Distribution Characteristics and Driving Factors of the Bacterial Community Structure in the Soil Profile of a Discontinuous Permafrost Region. Forests 2024, 15, 1456. https://doi.org/10.3390/f15081456
Liu Q, Song L, Zou S, Wu X, Zang S. Distribution Characteristics and Driving Factors of the Bacterial Community Structure in the Soil Profile of a Discontinuous Permafrost Region. Forests. 2024; 15(8):1456. https://doi.org/10.3390/f15081456
Chicago/Turabian StyleLiu, Qilong, Liquan Song, Siyuan Zou, Xiaodong Wu, and Shuying Zang. 2024. "Distribution Characteristics and Driving Factors of the Bacterial Community Structure in the Soil Profile of a Discontinuous Permafrost Region" Forests 15, no. 8: 1456. https://doi.org/10.3390/f15081456