Cellulolytic Bacteria Associated with the Gut of Dendroctonus armandi Larvae (Coleoptera: Curculionidae: Scolytinae)
Abstract
:1. Introduction
2. Experimental Section
2.1. Insect Collection and Dissection
2.2. Isolation and Counting of Cellulolytic Microorganisms
2.3. CMC Assay
2.4. ARDRA
2.5. Molecular and Phylogenetic Analysis
2.6. Operational Taxonomic Units (OTU)
2.7. Morphological and Physiological Characteristic Tests
3. Results and Discussion
Representative strains | Closest known taxa | Colony | Cell morphology | Pellicle | Flagella | Spore | G * | Numnero of isolates | |
---|---|---|---|---|---|---|---|---|---|
Medium 2a | Medium 2b | ||||||||
A | Serratia | Pink | Rods, 0.39–0.72 × 0.92–6.43 μm | + | Peritrichous | − | − | 2 | 5 |
B | Serratia | Pink | Rods, 0.31–0.66 × 0.96–5.54 μm | + | Peritrichous | − | − | 11 | 20 |
C | Pseudomonas | Ivory | Rods, 0.35–0.66 × 1.08–2.75 μm | − | ND | − | − | 10 | 10 |
D | Bacillus | Ivory | Rods, 0.38–0.67 × 0.84–3.24 μm | + | Peritrichous | + | + | 2 | 10 |
F | Paenibacillus | Pink | Rods, 0.64–1.32 × 2.12–6.10 μm | − | Peritrichous | + | + | 0 | 4 |
H | Sphingomonas | Yellow | Rods, 0.32–0.59 × 0.98–10.62 μm | − | Peritrichous | + | − | 0 | 1 |
I | Serratia | Pink | Rods, 0.36–0.70× 0.91–4.98 μm | + | Peritrichous | − | − | 2 | 5 |
J | Brevundimonas | Pale yellow | Rods, 0.61–1.13× 1.64–3.50 μm | − | Peritrichous | + | − | 0 | 2 |
M | Brevundimonas | Ivory | Rods, 0.28–0.50× 1.16–3.40 μm | − | Peritrichous | + | − | 2 | 1 |
N | Pseudoxanthomonas | Yellow | Rods, 0.21–0.38× 1.05–2.50 μm | + | Polar | − | − | 1 | 1 |
P | Methylobacterium | Pale yellow | Rods, 0.32–0.50× 0.89–2.40 μm | − | Polar or subpolar | − | − | 2 | 0 |
4. Conclusions
Acknowledgments
Author Contributions
Conflicts of Interest
References
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Hu, X.; Yu, J.; Wang, C.; Chen, H. Cellulolytic Bacteria Associated with the Gut of Dendroctonus armandi Larvae (Coleoptera: Curculionidae: Scolytinae). Forests 2014, 5, 455-465. https://doi.org/10.3390/f5030455
Hu X, Yu J, Wang C, Chen H. Cellulolytic Bacteria Associated with the Gut of Dendroctonus armandi Larvae (Coleoptera: Curculionidae: Scolytinae). Forests. 2014; 5(3):455-465. https://doi.org/10.3390/f5030455
Chicago/Turabian StyleHu, Xia, Jiamin Yu, Chunyan Wang, and Hui Chen. 2014. "Cellulolytic Bacteria Associated with the Gut of Dendroctonus armandi Larvae (Coleoptera: Curculionidae: Scolytinae)" Forests 5, no. 3: 455-465. https://doi.org/10.3390/f5030455