Next Article in Journal
Seasonal Variations of Carbon Dioxide, Water Vapor and Energy Fluxes in Tropical Indian Mangroves
Previous Article in Journal
Potential for Hybrid Poplar Riparian Buffers to Provide Ecosystem Services in Three Watersheds with Contrasting Agricultural Land Use
 
 
Font Type:
Arial Georgia Verdana
Font Size:
Aa Aa Aa
Line Spacing:
Column Width:
Background:
Article

Above- and Belowground Biomass Models for Trees in the Miombo Woodlands of Malawi

Department of Ecology and Natural Resource Management, Norwegian University of Life Sciences, P.O. Box 5003, 1432 Ås, Norway
*
Author to whom correspondence should be addressed.
Forests 2016, 7(2), 38; https://doi.org/10.3390/f7020038
Submission received: 25 October 2015 / Revised: 29 December 2015 / Accepted: 25 January 2016 / Published: 5 February 2016

Abstract

:
In this study we present general (multiple tree species from several sites) above- and belowground biomass models for trees in the miombo woodlands of Malawi. Such models are currently lacking in the country. The modelling was based on 74 trees comprising 33 different species with diameters at breast height (dbh) and total tree height (ht) ranging from 5.3 to 2 cm and from 3.0 to 25.0 m, respectively. Trees were collected from four silvicultural zones covering a wide range of conditions. We tested different models including dbh, ht and wood specific gravity ( ρ ) as independent variables. We evaluated model performance using pseudo-R2, root mean square error (RMSE), a covariance matrix for the parameter estimates, mean prediction error (MPE) and relative mean prediction error (MPE%). Computation of MPE% was based on leave-one-out cross-validation. Values of pseudo-R2 and MPE% ranged 0.82–0.97 and 0.9%–2.8%, respectively. Model performance indicated that the models can be used over a wide range of geographical and ecological conditions in Malawi.

1. Introduction

Miombo woodlands, classified as dry forests, are dominated by woody plants, primarily trees, whose canopy cover more than 10% of the ground surface, occurring in a climate with a dry season of three months or more [1]. The woodlands are dominated by deciduous trees of the genera Brachystegia, Julbernadia and Isoberlinia, which cover an area of approximately 2.7 million km2 spanning ten countries in eastern and central Africa including Malawi [1,2,3,4,5]. Miombo woodlands may be divided into dry and wet miombo. Dry miombo occur in areas receiving less than 1000 mm of rainfall annually in Zimbabwe, central Tanzania, and in the southern areas of Mozambique, Malawi and Zambia. Wet miombo occur in areas receiving more than 1000 mm of annual rainfall in eastern Angola, northern Zambia, south western Tanzania and central Malawi [1,6]. In Malawi, miombo woodlands constitute 92.4% of the country’s total forested area, and are mainly located in forest and game reserves established for water catchment as well as for soil and biodiversity conservation [2,7].
Miombo woodlands play a critical role in the livelihoods of Malawian communities because they provide social, economic, and environmental benefits, such as firewood, timber, medicinal plants, food, and catchment protection, among others [8]. Increased population growth, currently estimated at an annual growth rate of 2.8% [9], has led to higher demand for firewood, charcoal, and timber. Thus, the woodlands are being deforested at an annual rate of approximately 0.9%, which is among the highest rates in Africa [10].
To sustain the provision of these services, there is an urgent need to implement sustainable forest management measures including estimation of growing stock, productivity, forest biomass and yield [11,12]. Estimation of forest biomass is the first step towards calculation of carbon stocks. Due to the natural capacity of trees to sequester carbon dioxide, miombo woodlands are considered an important element in global climate change mitigation programs such as the reducing emissions from deforestation and forest degradation mechanism (REDD+), which provides a framework where developing countries may be financially rewarded for reducing carbon emissions.
The Malawi government recently established a baseline for forest biomass and carbon stock estimates for targeted forest reserves in miombo woodlands of Malawi [7]. However, the estimates are unreliable because of the nature of the allometric models that were used. For example, the aboveground forest biomass estimates were based on a pan-tropical biomass model developed by Chave et al. [13]. This model was developed using data from trees in tropical America and Asia but did not include Africa or the miombo woodlands. In addition, belowground forest biomass estimates were based on an allometric model developed by Cairns et al. [14]. Unlike Chave et al. [13], this dataset included some trees from Africa, i.e., Democratic Republic of the Congo, Ghana and the Ivory Coast. However, the trees were from moist evergreen tropical forests, whose structure is different from miombo woodlands.
By 2011 there were approximately 370 allometric models for predicting tree biomass in sub-Saharan Africa [15]. The majority of these models were developed for tropical rainforests in western Africa. Among the models in south-eastern Africa, only a few were developed for miombo woodlands. These models consisted of: (a) models developed for specific tree species based on a dataset from one site (Mwakalukwa et al. [16]); (b) models developed for specific tree species based on a dataset from several sites (Mate et al. [17]); (c) models developed for multiple tree species based on a dataset from one site (Chidumayo [18], Chamshama et al. [19], Malimbwi et al. [20], Ryan et al. [4], Mwakalukwa et al. [16]) and (d) models developed for multiple tree species based on a dataset from several sites (Mugasha et al. [21]).
Miombo woodlands are characterized by high tree species diversity, and the reported number of species from assessments at different spatial scales ranges from 80 to 300 [22,23,24,25,26,27]. Due to such large number of tree species, the applicability of species-specific models is limited. Furthermore, applicability of single-site models over different ecological zones is also limited due to their narrow geographical range. A scenario with general models, combining multiple species collected over several sites, would therefore be the best alternative, for example, in cases where national forest inventories are to be carried out. No such models exist for miombo woodlands in Malawi.
Most of the previously mentioned studies focused on aboveground biomass. However, estimation of belowground biomass in miombo woodlands is also vital. Belowground tree biomass, as a basis for model development, can be determined using complete excavation of roots, soil core sampling for fine and medium roots, and root sampling (complete excavation of a few sampled roots of a tree). Estimating belowground tree biomass can be done by using the root to shoot ratio (RS-ratio), i.e., the ratio between belowground and aboveground dry weights (see e.g., [28,29]), or through allometric models. Belowground biomass models for miombo woodlands in neighbouring countries were developed by Mugasha et al. [21], Chidumayo [18] and Ryan et al. [4].
The Inter-governmental Panel on Climate Change (IPCC) (see [30]) requires biomass and carbon reporting under the REDD+ mechanism to be accompanied by appropriate measures of uncertainty. Uncertainties are likely to occur in the following steps in biomass quantification: (i) when applying the sampling design (number and size of plots); (ii) during tree measurements and (iii) when applying the biomass model (e.g., [31,32]). The model-related uncertainty in this context stems from sources such as: (a) model misspecifications; (b) uncertainties in values of independent variables; (c) residual variability and (d) uncertainty in the model parameter estimates (see e.g., [33,34]). Among these, uncertainty in model parameter estimates has a great influence [35]. However, very few studies report uncertainty in the model parameter estimates, i.e., the covariance structure of the parameter estimates of developed models, and this makes it impossible to analyse the totality of the uncertainty related to estimated forest biomass (see [35]).
The objective of this study is to develop general (multiple tree species from several sites) above- and belowground biomass models applicable across the entire distribution of miombo woodlands in Malawi. The models are also accompanied with information on their covariance structure to enable quantification of model-related uncertainties in biomass and carbon estimation. Furthermore, we provide basic statistics on RS-ratios and compare the performance of our models with existing models from miombo woodlands.

2. Materials and Methods

2.1. Site Description

The sample trees for model development were selected from four forest reserves, namely Mtangatanga, Kongwe, Mua-livulezi and Tsamba (Figure 1, Table 1). The selection of sites was based on geographical location, management regime, silvicultural classification, and climatic conditions to capture a wide range of factors influencing tree growth [1].
Figure 1. Map of Malawi showing the location of the study sites.
Figure 1. Map of Malawi showing the location of the study sites.
Forests 07 00038 g001
Table 1. Geographical location, management regime, silvicultural classification and climatic conditions of study sites.
Table 1. Geographical location, management regime, silvicultural classification and climatic conditions of study sites.
MtangatangaKongweMua-livuleziTsamba
RegionNorthernCentralCentralSouthern
DistrictMzimbaDowaDedzaNeno
Location11°56′ S 33°42′ E13°35′ S 33°55′ E14°21′ S 34°37′ E15°21′ S 34°36′ E
Area (ha)84431813121473240
Management regimeCo-managementGovernmentCo-managementGovernment
Altitude (m)1500–17001000–1500400–900700–1500
Dominant soil typeHumic ferralliticFerruginousLithosolsFerrallitic
Silvicultural classificationMoist BrachystegiaMoist BrachystegiaDry BrachystegiaMoist Brachystegia
Mean minimum annual temp (°C)66138
Mean maximum annual temp (°C)29293228
Total annual rainfall range (mm)960–1050960–1050840–9601200–1600
Rain periodDecember–AprilNovember–AprilNovember–AprilNovember–April
Dry monthsMay–NovemberMay–OctoberMay–OctoberMay–October
Data sources: Rainfall and temperature data (1975–2005) from Ministry of Natural Resources, Energy and Mining, Department of Climate Change and Meteorological Services in Malawi. Soil and silvicultural classification according to Hardcastle [36].

2.2. Selection of Sample Trees

We conducted systematic sample plot inventories covering each site to collect information on ranges in tree size and species distribution to guide the selection of sample trees (e.g., [21]). We used circular plots with radius 11.28 m (400 m2). On each plot, we identified all tree species and measured their diameter at breast height (dbh) for all trees with dbh >4 cm. In addition, we sampled three trees within each plot, (one with the smallest, one with a medium and one with the largest dbh), and measured their total height (ht). The inventories covered a total of 221 plots with 70, 30, 71 and 50 plots for Mtangatanga, Kongwe, Mua-livulezi and Tsamba, respectively. The maximum recorded dbh values based on all sample plots in Mtangatanga, Kongwe, Mua-livulezi and Tsamba were 61 cm, 73 cm, 70 cm and 56 cm, respectively, while the number of species identified for the respective sites were 66, 45, 77 and 65. In total, for all the study sites, we identified 139 species. The most frequent species for Mtangatanga, Kongwe, Mua-livulezi and Tsamba were Uapaca kirkiana Müll. Arg., Brachystegia spiciformis Benth., Diplorhynchus condylocarpon (Müll. Arg.) Pichon and Uapaca kirkiana Müll. Arg, respectively.
A total of 74 trees were selected based on the observed dbh and tree species frequency within the sites. We ensured that the trees were selected from all dbh classes observed in the sample plot inventories. In addition, we selected a total of eight trees with larger dbh than those observed in the sample plot inventories to reduce uncertainty when predicting biomass of very large trees. We also selected at least one tree among the eight most frequent species observed in each site. The remaining sample trees were selected randomly among all species. In total, 33 tree species were selected, comprising 10, 10, 12 and 10 different tree species in Mtangatanga, Kongwe, Mua-livulezi and Tsamba, respectively.
Before felling (at a stump height of 30 cm), we recorded scientific and local names, and measured dbh, stump diameter (at 30 cm above ground) and ht (Table 2 and Table A1). We used either a calliper or a diameter tape, depending on tree sizes, to measure dbh and stump diameter, while a Suunto hypsometer was used for all ht measurements. These trees have previously been used to develop general volume models for miombo woodlands in Malawi [37].
Table 2. Mean, minimum, maximum and standard deviation (STD) of diameter at breast height (dbh) and total tree height (ht) for sample trees at each site.
Table 2. Mean, minimum, maximum and standard deviation (STD) of diameter at breast height (dbh) and total tree height (ht) for sample trees at each site.
SiteNo. of Treesdbh (cm)ht (m)
MeanMin.Max.STDMeanMin.Max.STD
Mtangatanga2035.56.0111.226.710.74.018.04.3
Kongwe1834.99.075.719.611.75.022.04.7
Tsamba1830.28.475.017.512.66.525.05.1
Mua-livulezi1832.85.381.723.011.63.022.05.8
All7433.45.3111.221.811.63.025.04.9

2.3. Destructive Sampling

We separated each sample tree into the following aboveground components: merchantable stem (from the stump at 30 cm above ground to the point where the first branches start), branches (all parts of the tree above the defined merchantable stem and up to a minimum diameter of 2.5 cm) and twigs (all branches with a diameter less than 2.5 cm). For small trees not considered suitable for timber production (dbh < 15 cm, in total 14 trees), merchantable stem biomass were allocated to branches (e.g., [37,38]). During the work on destructive sampling most of the trees had already started to shed leaves. We therefore excluded leaves from twigs, and leaves were not included in the models.
To facilitate measurements, stems and branches were crosscut into manageable pieces of approximately 1–2 m in length and then weighed for fresh weight using a mechanical hanging spring balance (0–200 kg). Twigs from each tree were separately bundled and weighed for fresh weight. Three small sub-samples, varying in weight between 0.1 and 1.0 kg, from each of the components (merchantable stem, branches and twigs) were taken from each sample tree and weighed with an electronic balance for fresh weight and finally brought to laboratory for drying. The sub-samples were taken from the biggest, medium and smallest diameter parts of each tree component.
For determination of belowground biomass of the sample trees, our strategy involved root sampling at two levels, namely main roots (roots branching directly from the root crown) and side roots (roots branching from the main roots). The first step in excavation involved clearing the topsoil around the tree base to expose the points at which the roots were branching. We then selected three main roots, i.e., the main roots with the largest, medium and smallest diameters, and recorded their diameters at the points where they joined the root crown. The diameters of all main roots not excavated were recorded at the point where they were joined the root crown. From each of the selected main roots, we selected up to three side roots, i.e., the side roots with the largest, medium and smallest diameters. For each of the selected side roots, we recorded the diameter where they joined the main root. For the remaining side roots, we also recorded the diameters at the branching point from the main root. The selected side and main roots were then fully excavated up to the minimum diameter of 1 cm and then weighed. In cases where the full roots could not be excavated due to obstacles such as rocks, the diameter of the last bit of the root was recorded and we treated the remaining unexcavated part as a side root. An effort was made to ensure that all the main, side and taproots were fully excavated up to the last 1 cm. In total, 38 out of the 41 trees, had taproots. Out of the 38 trees, we were not able to fully excavate the taproots for 16 trees. In such cases, the diameter at the breaking point of the unexcavated taproot was recorded and treated as a side root. On average, trees were dug down to 2.5 m depth. Lastly, we recorded the fresh weight of the root crown for each tree.
For all sample trees, three small sub-samples, varying in weight between 0.1 and 1.0 kg, were taken from each main and side root, and one was taken from the root crown. We obtained the fresh weight of the sub-samples using an electronic balance and brought them to the laboratory for drying.

2.4. Laboratory Analyses and Determination of Biomass Dry Weight

All sub-samples, from both above- and belowground, for each tree were dried in an oven in a laboratory at a temperature of 80 °C until a constant weight was achieved (constant weight was observed in 2–3 days). We then recorded dry weights of the individual sub-samples. Subsequently, we used the sub-sample dry and fresh weights to determine the tree- and section specific dry to fresh weight ratios (DF-ratios) (see Table A2).
We then calculated the dry weight of each section as a product of tree- and section specific DF-ratios and the fresh weights of the respective trees and tree sections. Subsequently, we computed the total aboveground dry weight by summing the dry weights of the merchantable stem, branches and twigs of each tree (Figure 2, Table A1).
Figure 2. Total aboveground tree biomass (kg dry weight) distribution over dbh (cm) and ht (m) for Mtangatanga, Kongwe, Mua-livulezi and Tsamba and forest reserves.
Figure 2. Total aboveground tree biomass (kg dry weight) distribution over dbh (cm) and ht (m) for Mtangatanga, Kongwe, Mua-livulezi and Tsamba and forest reserves.
Forests 07 00038 g002
To determine the total belowground dry weights of the excavated parts of the trees we first converted all the fresh weights from the different sections to dry weight biomass by multiplying the tree- and section specific DF-ratios and their respective fresh weights. We then developed a general (combining data from all sites) side root model by regressing the dry weight biomass of the fully excavated side roots and their diameters (cm). We assumed the relationship between side root biomass and root diameter (similarly for main roots, see below) to exhibit a power-law relationship described as:
B = a   × d b
where B = dry weight biomass of a side root or main root (kg); d = diameter (cm) of a side or main root at the point it is joining the main root or the root crown, respectively; a and b are parameter estimates. The following side root model was developed:
B =   0.198102 × d 1.656968 ,   Pseudo - R 2 = 0.67 ,   MPE % =   2.0
where
Pseudo - R 2 =   1   ( SSR CSST )
SSR is the sum of squared residuals and CSST is the corrected total sum of squares. The mean prediction error (MPE), and the relative mean prediction error (MPE%) is calculated as
MPE =   i = 1 n ( y i y ^ i ) n ,   MPE % =   MPE y ¯   × 100
where y i is the observed biomass of tree i, y ^ i   is the predicted biomass of tree i and y ¯ is the mean observed biomass. Both MPE and MPE% are based on leave-one-out-cross validation. The MPE% value for the side root model was not significantly different from zero indicating appropriate model performance.
The side root model was used to predict the dry weight biomass of all the side roots that were not excavated for the main sample root. The total dry weight of all side roots for each main sample root was then determined by summing dry weights of the excavated side roots and predicted dry weights of unexcavated side roots. Finally the complete dry weight of the sample main root was determined by summing the total dry weights of all side roots and the excavated parts of the main root. The following main root model was then developed and applied to predict the dry weights of main roots not excavated;
B =   0.063132 × d 2.174388 ,   Pseudo - R 2 = 0.79 ,   MPE % =   0.4
The MPE% value for the main root model was not significantly different from zero indicating appropriate model performance. To determine the dry weight of unexcavated parts of the taproots (16 trees), we applied the general side root model.
Total belowground dry weight biomass for each tree was finally determined by adding the dry weights of all excavated and unexcavated main roots, dry weight of the taproot, and the dry weight of the root crown (Figure 3, Table A1).
Figure 3. Total belowground tree biomass (kg dry weight) distribution over dbh (cm) and ht (m) for Mtangatanga, Kongwe, Mua-livulezi and Tsamba and forest reserves.
Figure 3. Total belowground tree biomass (kg dry weight) distribution over dbh (cm) and ht (m) for Mtangatanga, Kongwe, Mua-livulezi and Tsamba and forest reserves.
Forests 07 00038 g003aForests 07 00038 g003b

2.5. Model Development and Evaluation

Before fitting the models, we assessed the basic diagnostic plots of biomass over dbh and ht. As expected, the plots indicated non-linear patterns in the relationships between biomass and dbh and ht (Figure 2 and Figure 3). Since wood specific gravity ( ρ ) is considered as an important factor for explaining variation in biomass (e.g., [39]), we included this variable in the models. We therefore tested the following models:
Model   1 :   B = a × dbh b
Model   2 :   B = a × dbh b × ht c
Model   3 :   B = a × dbh b × ρ c
Model   4 :   B = a × dbh b × ht c × ρ d
where B is biomass (kg), dbh is diameter at breast height (cm), ht is total tree height (m) and ρ is the species-specific mean wood specific gravity (g/cm3) extracted from the global wood density database [40,41] and a, b, c and d are parameter estimates.
Since the data was collected from different study sites in different geographical regions and silvicultural zones across Malawi, we anticipated that individual tree attributes would be different depending on site. We therefore initially fitted mixed effects models, with site as a random effect, to the side root, main root and total belowground and aboveground biomass datasets using PROC NLMIXED of SAS 9.4 [42]. This procedure fits nonlinear mixed models, that is, models in which both fixed and random effects enter nonlinearly. PROC NLMIXED fits nonlinear mixed models by maximizing an approximation to the likelihood integrated over the random effects using the maximum likelihood estimation method.
These mixed effects models were compared with weighted nonlinear regression models fitted using PROC MODEL in SAS 9.4 [42]. This procedure fits models in which the relationships among the variables comprise a system of one or more nonlinear equations using the full information maximum likelihood (FIML) estimation method.
For each model derived from the two procedures, i.e., mixed effects and weighted regression, we computed Akaike Information Criterion (AIC) values [43]. AIC measures the model goodness-of-fit whilst correcting for model complexity. We used the AIC values to compare mixed effects models with the weighted regression nonlinear models. The results showed that, in all cases, weighted regression models produced lower AIC values relative to mixed effects models. We thus decided to develop our final models based on weighted regression.
Model efficiency and performance were assessed based on results from a leave-one-out cross validation procedure [44]. This splits the dataset of n observations into two parts, namely, a validation dataset and a training dataset. The validation dataset comprises a single observation (x1, y1) and the training dataset comprises the remaining {(x2, y2),….., (xn, yn)} observations. The model is fitted on the n-1 observations in the training dataset and a prediction y   ^ is made for a single observation in the validation dataset, using its value x1. Since (x1, y1) was not used in the fitting process, the square error (SE) = (y1 y ^ )2 provides an estimate of the test error. This procedure is repeated n times, thus producing n test errors, SE1…….SEn. The leave-one-out-cross validation estimate for the test error is the mean of these n test error estimates (MSE).
The cross validation results were then used to calculate the root mean square error (RMSE) as follows;
RMSE = MSE
RMSE  ( % ) = RMSE y ¯ × 100
where y ¯ is the mean observed biomass and RMSE (%) is the relative Root Mean Square Error.
Model comparison was based on AIC values. Models with insignificant parameter estimates were not considered irrespective of AIC values. For all the models, we presented pseudo-R2, RMSE, RMSE (%), covariance matrix for the parameter estimates, and the MPE and MPE% values based on leave-one-out cross validation. Student t-tests were conducted to determine whether the MPE values were significantly different from zero.
In addition, we tested a number of previously developed biomass models (Table 3) on our data. This included models developed for miombo woodlands in neighbouring countries, i.e., Ryan et al. [4] in Mozambique, Mugasha et al. [21] in Tanzania and Chidumayo [18] in Zambia, and the pan-tropical model developed by Chave et al. [39]. MPE values were computed, and student t-tests were applied to determine whether the MPE values were significantly different from zero.
For a graphical display of the behaviour of models with ht as independent variable, i.e., Mugasha et al. [21] and Chave et al. [39] (see Table 3), we applied a height-diameter model developed from our sample trees:
ht = 1.3 + exp 3.787685 6.62809   ×   dbh 0.45222
Furthermore, when applying Chave et al. [39], we extracted ρ values from the global wood density database [40,41] and subsequently calculated a mean ρ value, which was then used for the graphical display of this model.
Table 3. Number of sites, sample trees and dbh ranges (cm) of previously developed models tested on our data.
Table 3. Number of sites, sample trees and dbh ranges (cm) of previously developed models tested on our data.
Tree SectionAuthorModelNo. of SitesNo. of Treesdbh Range (cm)Species
Above-GroundMugasha et al. [21] B = 0.1027 × dbh 2.4798 41671.1–11060
Mugasha et al. [21] B = 0.0763 × dbh 2.2046 × ht 0.4918 41671.1–11060
Ryan et al. [4] a C = 3.629 + 2.601 × log ( dbh ) 1295–736
Chidumayo [18] B = 2.5265 + 2.5553 × log ( dbh ) 11132–3919
Chave et al. [39] b B = 0.0673 × ( ρ × dbh 2 × ht ) 0.976 5840045–180Unclear
Below-GroundMugasha et al. [21] B = 0.2113 × dbh 1.9838 4803.3–9560
Mugasha et al. [21] B = 0.1766 × dbh 1.7844 × ht 0.3434 4803.3–9560
Ryan et al. [4] a C = 3.370 + 2.262 × log ( dbh ) 1235–726
Chidumayo [18] B = 1.9439 + 2.1712 × log ( dbh ) 1124–3519
a Carbon converted to biomass as B = ( C 47 )     × 100 ; b ρ (g/cm3) is the species-specific mean wood specific gravity (g/cm3) extracted from the global wood density database [40,41].

3. Results

The mean RS-ratio of the 41 trees sampled both above- and belowground was 0.47 (Table 4). No significant differences in RS-ratios were found between sites (p = 0.8684, F = 0.2400). The RS-ratio decreased nonlinearly with increasing dbh (Figure 4).
Table 4. Mean, standard deviation (STD) and range of root to shoot ratios (RS-ratio) over sites.
Table 4. Mean, standard deviation (STD) and range of root to shoot ratios (RS-ratio) over sites.
SiteNo. of TreesMeanMinMaxSTD
Mtangatanga120.490.321.150.25
Kongwe100.440.220.910.22
Mua-livulezi90.510.270.920.27
Tsamba100.440.210.780.16
All410.470.181.150.22
Figure 4. Root to shoot ratio (RS-ratio) vs. diameter at breast height (dbh). The dots represent observations for individual trees and the line represents the fitted nonlinear model.
Figure 4. Root to shoot ratio (RS-ratio) vs. diameter at breast height (dbh). The dots represent observations for individual trees and the line represents the fitted nonlinear model.
Forests 07 00038 g004
For aboveground biomass, all models, except Model 4, had significant parameter estimates and appropriate performance criteria, i.e., none of the models had MPE% values significantly different from zero (p > 0.05) (Table 5). Among these models, Model 2 with dbh and ht as independent variables had the smallest AIC value. The pseudo-R2 values for all models ranged from 0.93 to 0.97. For belowground biomass, Model 1 was the only one where all parameter estimates were significant. Covariance matrices for all models with significant parameter estimates in Table 5 are shown in Table A3.
Among the models with significant parameter estimates for twigs, branches and merchantable stem biomass (Table 6), Models 1, 2 and 2, respectively, provided the smallest AIC values. The pseudo-R2 values for the twigs, branches and merchantable stem models with significant parameter estimates were 0.82, 0.91–0.92 and 0.77–0.88, respectively.
We further evaluated the above- and belowground biomass models over sites and dbh classes (Table 7). None of the tested models produced MPE values significantly different from zero (p > 0.05) overall or for any site. However, a significant MPE was observed for dbh class 0–20 cm for Model 3. For the aboveground biomass models, MPE% values ranged from 0.4% to 15.1% while for the belowground biomass model, the MPE% values ranged from 2.1% to 3.9%.
Finally, we tested previously developed models (Table 3) on our dataset (Table 8). The MPE% when applying the aboveground biomass models developed by Mugasha et al. [21], Ryan et al. [4], Chidumayo [18] and Chave et al. [39] ranged from 2.8 to 30.8 (under prediction). The above- and belowground biomass models developed by Chidumayo [18] generally produced the lowest MPE% values, i.e., 2.8% and −4.7%, respectively. Figure 5 and Figure 6 display above- and belowground biomass over dbh for some of the models developed in the current study and some from the previous studies.
Table 5. Model parameters and performance criteria of above- and belowground biomass models.
Table 5. Model parameters and performance criteria of above- and belowground biomass models.
ComponentModel No.No. of TreesModelPseudo-R2RMSEMPEAIC
(kg)(%)(kg)(%)
Aboveground174 0.21691 × dbh 2.318391 0.93751.260.6−22.2−1.8981.89
274 0.103685   ×   d b h 1.921719 ×   h t 0.844561     0.97426.634.4−19.8−1.6954.27
374 0.290457 × dbh 2.283998 × ρ 0.443619 0.94923.174.5−53.2−4.3977.05
474 0.129899 × dbh 1.90203 × ht 0.828647 × ρ 0.296271 NS 0.97542.143.7−37.1−3.0952.35
Belowground141 0.284615 ×   d b h 1.992658     0.94161.730.7−4.8−0.9481.23
241 0.224132 × dbh 1.899061 × ht 0.222554 NS 0.94169.732.2−7.4−1.4481.73
341 0.415451 × dbh 1.933905 × ρ 0.465663 NS 0.94175.433.3−5.0−1.0481.74
441 0.350488 × dbh 1.732452 × ht 0.395127 NS × ρ 0.836154 NS 0.94170.232.3−6.5−1.2479.66
NS Parameter estimate not significant (p > 0.05). Note: biomass in kg, dbh in cm, ht in m, and ρ in g/cm3, Bold: best model according to AIC (Akaike Information Criterion (AIC)).
Table 6. Model and performance criteria for twigs, branches and merchantable stem biomass models.
Table 6. Model and performance criteria for twigs, branches and merchantable stem biomass models.
ComponentModel No.No. of TreesModelPseudo-R2RMSEMPEAIC
(kg)(%)(kg)(%)
Twigs172 0.07239   ×   d b h 1.858897 0.8239.362.5−0.8−1.2602.84
272 0.070224 × dbh 1.598204 × ht 0.384139 NS 0.8437.860.1−1.1−1.7634.09
372 0.130116 × dbh 1.764995 × ρ 0.687581 NS 0.8344.370.4−1.4−2.2616.35
472 0.109969 NS × dbh 1.446488 × ht 0.549901 × ρ 0.951968 0.8340.263.9−1.5−2.4631.63
Branches174 0.137316 × dbh 2.328104 0.91659.880.9−25.4−3.1973.58
274 0.051157   ×   d b h 2.161115 ×   h t 0.598879     0.92565.269.3−22.6−2.8933.50
374 0.123375 × dbh 2.379626 × ρ 0.30643 NS 0.91789.096.8−47.5−5.8946.79
474 0.059792 × dbh 2.150762 × ht 0.582627 × ρ 0.20053 NS 0.92692.284.9−39.3−4.8934.74
Merchantable160 0.145576 × dbh 2.116265 0.77299.966.9−1.2−0.3773.89
Stem260 0.030811 ×   d b h 1.572422 ×   h t 1.345696 0.88249.955.85.31.2737.16
360 0.221213 × dbh 2.059249 × ρ 0.544483 0.79330.273.7−13.9−3.1772.48
460 0.039194 × dbh 1.554911 × ht 1.329832 × ρ 0.36722 NS 0.88256.257.22.60.6737.75
NS Parameter estimate not significant (p > 0.05). Note: biomass in kg, dbh in cm, ht in m, and ρ in g/cm3. Bold: best model according to AIC.
Table 7. Mean prediction errors (MPE) of the models over study sites and dbh classes.
Table 7. Mean prediction errors (MPE) of the models over study sites and dbh classes.
ComponentModel No.Variable No. of TreesObservedPredictedMPE
(kg)(kg)(kg)(%)
Aboveground1SiteMtangatanga201465.51606.2−140.8−9.6
Kongwe181195.41200.6−5.2−0.4
Mua-livulezi181316.71231.585.36.5
Tsamba18956.3882.473.87.7
dbh class0–202179.793.4−13.6−17.1
(cm)21–4035545.6584.1−38.5−7.1
41–6062225.41911.6313.914.1
>60124801.54826.0−24.5−0.5
All 741239.71240.3−0.6−0.1
2SiteMtangatanga201465.51424.141.42.8
Kongwe181195.41146.848.64.1
Mua-livulezi181316.71337.8−21.1−1.6
Tsamba18956.31032.2−75.9−7.9
dbh class0–202179.786.3−6.5−8.2
(cm)21–4035545.6578.5−32.9−6.0
41–6062225.42111.9112.55.1
>60124801.54754.147.41.0
All 741239.71240.3−0.6−0.1
3SiteMtangatanga201465.51686.3−220.8−15.1
Kongwe181195.41133.362.15.2
Mua-livulezi181316.71205.4111.48.5
Tsamba18956.3889.367.07.0
dbh class0–202179.796.3−16.5−20.7 *
(cm)21–4035545.6585.6−40.0−7.3
41–6062225.41936.4289.113.0
>60124801.54807.8−6.3−0.1
All 741239.71240.9−1.2−0.1
Belowground1SiteMtangatanga12795.9777.018.92.4
Kongwe10386.3401.4−15.1−3.9
Mua-livulezi9427.7418.98.82.1
Tsamba10435.3450.9−15.5−3.6
dbh class0–201242.146.1−4.0−9.5
(cm)21–4016240.3259.3−19.0−7.9
41–604821.3736.984.410.3
>6091553.41551.91.50.1
All 41527.2527.2−0.0−0.0
* MPE is significantly different from zero (p < 0.05).
Table 8. Mean prediction error (MPE) of previously developed models.
Table 8. Mean prediction error (MPE) of previously developed models.
ComponentModelIndependent Variable(s)No. of TreesObservedPredictedMPE
(kg)(kg)(kg)(%)
AbovegroundMugasha et al. [21]dbh741239.71135.7104.08.4
Mugasha et al. [21]dbh, ht741239.71076.7163.013.2 **
Ryan et al. [4]dbh741239.71068.8170.913.8 *
Chidumayo [18]dbh741239.71205.634.12.8
Chave et al. [39]dbh, ρ , ht741239.7953.7286.123.1 ***
BelowgroundMugasha et al. [21]dbh41527.2377.5149.728.4 ***
Mugasha et al. [21]dbh, ht41527.2364.8162.430.8 ***
Ryan et al. [4]dbh41527.2426.9100.319.0 ***
Chidumayo [18]dbh41527.2551.9−24.7−4.7
* MPE is significantly different from zero at (p < 0.05); ** MPE is significantly different from zero at (p < 0.01) and *** MPE is significantly different from zero at (p < 0.001)
Figure 5. Aboveground biomass (dry weight) over dbh based on the general models developed in this study (with dbh and ht as independent variables), by Mugasha et al. [21] (with dbh and ht as independent variables), by Ryan et al. [4] (with dbh as only independent variable), by Chidumayo [18] (with dbh only as independent variable) and by Chave et al. [39] (with dbh, ρ , and ht as independent variables). CHI, RY, MG and CS are the maximum dbh values for the data used in the models developed by Chidumayo [18], Ryan et al. [4], Mugasha et al. [21] and current study, respectively.
Figure 5. Aboveground biomass (dry weight) over dbh based on the general models developed in this study (with dbh and ht as independent variables), by Mugasha et al. [21] (with dbh and ht as independent variables), by Ryan et al. [4] (with dbh as only independent variable), by Chidumayo [18] (with dbh only as independent variable) and by Chave et al. [39] (with dbh, ρ , and ht as independent variables). CHI, RY, MG and CS are the maximum dbh values for the data used in the models developed by Chidumayo [18], Ryan et al. [4], Mugasha et al. [21] and current study, respectively.
Forests 07 00038 g005
Figure 6. Belowground biomass (dry weight) over dbh based on the general models developed in this study, by Mugasha et al. [21], by Ryan et al. [4] and by Chidumayo [18]. All models had dbh as the only independent variable. CHI, RY, MG and CS are the maximum dbh values for the data used in the models developed by Chidumayo [18], Ryan et al. [4], Mugasha et al. [21] and current study, respectively.
Figure 6. Belowground biomass (dry weight) over dbh based on the general models developed in this study, by Mugasha et al. [21], by Ryan et al. [4] and by Chidumayo [18]. All models had dbh as the only independent variable. CHI, RY, MG and CS are the maximum dbh values for the data used in the models developed by Chidumayo [18], Ryan et al. [4], Mugasha et al. [21] and current study, respectively.
Forests 07 00038 g006

4. Discussion

Capturing a wide range of natural variability in factors influencing tree growth, such as soil types, temperature, and rainfall, is important for developing robust biomass models [21,39]. The modelling dataset for this study was collected from sites located in all the three regions of Malawi, i.e., north, central and south (Figure 1). Application of sound sampling procedures when selecting sample trees is also critical in development of models as it may help in reducing the probability of biases. In this study, we selected trees based on information from forest inventories for each site done prior to tree selection. We also included a number of very large trees to avoid extrapolation beyond the data ranges as much as possible. Our sample trees comprised 33 out of the 139 tree species identified during the forest inventories for the four study sites. Compared to most previous studies our dataset included a large number of tree species. The modelling dataset of Mugasha et al. [21] comprised 60 tree species, while those of Chidumayo [18] and Ryan et al. [4] had 19 and 6 species, respectively. Although the proportion of the number of tree species in our dataset is relatively low compared to total number of tree species reported in miombo woodlands [22,23,24,25,26,27], the information from the prior forest inventories ensured that the most common species were represented, in addition to species selected randomly among the remaining less frequent species.
Leaves and fine roots were excluded from our biomass sampling. Leaves were excluded because most of the trees had started to shed leaves when we carried out destructive sampling. This is a common challenge for biomass studies in seasonally dry forests, as acknowledged by Chave et al. [39]. A recent study on miombo woodlands in Mozambique by Mate et al. [17] found that leaves comprised only 3% of the total aboveground biomass during the peak leaf season. Such a number would probably be a good estimate of how much aboveground biomass is missing in our data.
Root parts with diameter < 1 cm were also excluded from the biomass data, mainly to reduce workload. Similar root parts (diameter < 2 cm) were also excluded in the Chidumayo [18] and Ryan et al. [4] models. Chidumayo [18] analysed cumulative root biomass vs. diameter and found that the trend levelled off considerably around a diameter of 2 cm. Thus, the underestimation of the belowground biomass in our data is small, especially since we used a diameter threshold of 1 cm.
RS ratio is an important alternative for estimating belowground tree biomass in cases where allometric models are not available (see [14,45]). The mean RS ratio observed here (0.47, Table 4) is higher than the value reported by Mugasha et al. [21] and Ryan et al. [4] for miombo woodlands (0.40 and 0.42 respectively). However, Chidumayo [18] reported a mean value of 0.54 which is comparatively higher. Since the RS ratio decreases with increasing dbh (Figure 4), the mean value depends on the size distribution of the trees. The high mean RS-ratio found by Chidumayo [18] is likely to result from the relatively smaller tree sizes in his dataset (see Table 3) rather than a higher proportion of root biomass in trees from miombo woodlands in Zambia. Mean RS ratios are frequently used to estimate belowground biomass (e.g., [46]). However, by using a fixed mean RS ratio for a relationship that most probably is non-linear (Figure 4), a bias will be introduced (see e.g., [47]). Therefore, application of mean RS-ratios to estimate belowground should be done with caution.
Among the aboveground biomass models with significant parameter estimates, Model 2 (with dbh and ht as independent variables) had the smallest AIC value (Table 5). Models with both dbh and ht as independent variables had a better fit than those with only dbh (e.g., [21,39]). Inclusion of ρ as independent variable (Model 3), in place of ht (Model 2), did not improve aboveground biomass prediction. This could be attributed to the fact that the ρ values were not obtained directly from the sampled trees, but from the global wood density database [40,41]. The model with the smallest AIC value (Model 2) is similar (pseudo-R2 = 0.97) to the models developed by Mugasha et al. [21] (pseudo-R2 = 0.95), Chidumayo [18] (R2 = 0.98) and Ryan et al. [4] (adjusted-R2 = 0.93).
Although Model 2 is generally considered the best aboveground biomass model, it should be noted that Models 1 and 3 can still be applied during forest inventories in cases where ht is lacking or considered as inaccurate. Application of Model 2 requires measuring both dbh and ht during a forest inventory. In such cases, ht for individual trees is usually predicted based on dbh-ht models developed from sample trees collected from the study site because measuring ht in all trees is too time consuming. However, measuring tree height is also prone to errors especially in closed-canopy forests, due to differences in crown shapes and the difficulty of observing the top of the tree crown [48,49].
Among the belowground biomass models, the only viable model, i.e., with significant parameter estimates, was the one with dbh as an independent variable (Model 1). The fit of this model is similar (pseudo-R2 = 0.94) to that of the models developed by Mugasha et al. [21] (pseudo-R2 = 0.92), Chidumayo [18] (R2 = 0.95) and Ryan et al. [4] (adjusted-R2 = 0.94).
Proper implementation of the REDD+ mechanism in participating countries, including Malawi, requires biomass estimates to be accompanied with an estimate of uncertainty (see [30]). Uncertainty of biomass estimates is usually computed from error estimates of model parameters for the employed biomass models [34]. In Table A3, we have therefore presented the covariance matrices of model parameters for all the valid models in Table 5 to enable potential users to estimate uncertainty in biomass and carbon quantities during national forest inventories and monitoring, reporting and verification systems under the REDD+ mechanism (see [30,34]).
Tree component biomass models, i.e., models for twigs, branches and merchantable stem, may be useful when planning commercial extraction of timber or quantification of biomass for domestic fuelwood or charcoal production (see [37]). All tree component models with significant parameter estimates produced MPE% values not significantly different from zero (see Table 6). This is an indication of appropriate performance.
The evaluation of the developed above- and belowground models on our own data showed that no models produced MPE% significantly different from zero for any site (Table 7), thus indicating that the models can be applied over a wide range of conditions. The trend was the same over dbh classes except for the smallest dbh class under Model 3. It should be noted that the magnitude of MPE% seen over sites in Table 7 is the kind of error that should be expected across sites if we were to apply our models across Malawi, e.g., in a national forest inventory.
The previously developed models from neighbouring countries resulted in large prediction errors significantly different from zero when applied to our data (Table 8). Exceptions were observed in the aboveground biomass models of Mugasha et al. [21] and the above- and belowground models of Chidumayo [18]. For the recently developed pan tropical aboveground biomass model of Chave et al. [39], the prediction error was also large (23.1% underestimation). The generally large prediction errors when appliying the previously developed models are, of course, not surprising since they are applied outside their respective data ranges. These results, however, also demonstrate the importance of developing local models.

5. Conclusions

We developed general above- and belowground, stem, branch and twig biomass models for the miombo woodlands of Malawi. Our models can be used over a wide range of geographical and ecological conditions in Malawi. The generally large prediction errors seen when applying previously developed models from neighbouring countries to our data demonstrated the importance of developing local models.

Acknowledgments

The first author of the study was mainly funded by the Norwegian Government through the Norwegian Quota Scheme. Special thanks should also go to the Norwegian University of Life Sciences and World Wildlife Fund (WWF) under the Russel E. Train Education for Nature Fellowship for funding the fieldwork. We would also like to thank the Forest Research Institute of Malawi for facilitating the grant of a research permit from the National Research Council of Malawi. We thank Wilson Mugasha, Norwegian University of Life Sciences, for his guidance during initial stages of fieldwork. Our gratitude is also extended to Devey Lekapo, Happy Kachamba, Humphrey Chapama, Boyd Zulu, Kola Daitoni and Steve Hara for working tirelessly during data collection. Finally, we also like to say thank you to District Forest Officers for Mzimba, Dowa, Dedza and Neno districts for their cooperation and guidance during data collection.

Author Contributions

Daud Jones Kachamba was responsible for developing the research design, data collection and analyses and manuscript development. Tron Eid guided the development of the research design and analyses, and participated in manuscript development. Terje Gobakken participated in manuscript development.

Conflicts of Interest

The authors declare no conflict of interest.

Appendix

Table A1. Dataset used for model development.
Table A1. Dataset used for model development.
SiteScientific NameLocal Namedbh (cm)ht (m)Total Above-Ground Biomass (kg)Total Below-Ground Biomass (kg)Use
MtangatangaBrachystegia glaucescens Burtt Davy & Hutch.Musani6.04.415.517.8a,b
Erica benguelensis (Welw. ex Engl.) E.G.H. Oliv.Msankhanya9.44.018.76.2a,b
Erica benguelensis (Welw. ex Engl.) E.G.H. Oliv.Msankhanya13.06.031.6N/Aa
Uapaca kirkiana Müll. Arg.Msuku15.05.073.436.8a,b
Julbernadia paniculata (Benth.) TroupinMtondo16.58.0106.084.2a,b
Isoberlinia angolensis (Benth.) Hoyle & BrenanKabale19.412.0266.386.5a,b
Brachystegia boehmii Taub.Mombo20.28.0194.0N/Aa
Uapaca kirkiana Müll. Arg.Msuku21.07.8175.2N/Aa
Brachystegia utilis Burtt Davy & Hutch.Nzale23.010.0278.8N/Aa
Brachystegia taxifolia Burtt Davy & Hutch.Mchinji23.610.0271.8N/Aa
Julbernadia paniculata (Benth.) TroupinMtondo27.310.0412.2N/Aa
Isoberlinia angolensis (Benth.) Hoyle & BrenanKabale28.611.0445.0N/Aa
Brachystegia spiciformis Benth.Chumbe36.012.0921.1N/Aa
Brachystegia longifolia Benth.Sanga36.010.81173.0511.1a,b
Brachystegia glaucescens Burtt Davy & Hutch.Musani48.414.51847.1947.1a,b
Brachystegia glaucescens Burtt Davy & Hutch.Musani50.016.02160.7741.5a,b
Brachystegia spiciformis Benth.Chumbe60.018.03935.01247.0a,b
Brachystegia glaucescens Burtt Davy & Hutch.Musani68.010.53233.21160.7a,b
Brachystegia glaucescens Burtt Davy & Hutch.Musani78.017.04952.11567.2a,b
Brachystegia glaucescens Burtt Davy & Hutch.Musani111.218.08798.53144.9a,b
KongweJulbernadia paniculata (Benth.) TroupinMtondo9.05.023.411.1a,b
Uapaca kirkiana Müll. Arg.Msuku10.25.026.2N/Aa
Brachystegia spiciformis Benth.Chumbe13.06.094.986.4a,b
Brachystegia manga De Wild.Mpapa/Bovo17.310.0138.485.7a,b
Julbernadia paniculata (Benth.) TroupinMtondo21.011.0264.9154.3a,b
Brachystegia utilis Burtt Davy & Hutch.Nzale24.59.0368.682.1a,b
Monotes africanus A. DC.Mkalakate27.210.0437.2N/Aa
Brachystegia spiciformis Benth.Chumbe27.59.3499.9N/Aa
Brachystegia boehmii Taub.Mombo30.814.0636.5N/Aa
Uapaca kirkiana Müll. Arg.Msuku34.012.0689.6201.4a,b
Parinari curatellifolia Planch. ex Benth.Muula37.312.0839.7285.7a,b
Brachystegia manga De Wild.Mpapa/Bovo38.117.01445.1N/Aa
Cussonia arborea Hochst. ex A. Rich.Mbwabwa38.47.0346.6N/Aa
Brachystegia spiciformis Benth.Chumbe38.714.01298.4492.7a,b
Brachystegia manga De Wild.Mpapa/Bovo52.022.02534.0N/Aa
Brachystegia manga De Wild.Mpapa/Bovo61.717.04400.31235.3a,b
Brachystegia spiciformis Benth.Chumbe71.218.04914.11228.4a,b
Erythrina abyssinica Lam. ex DC.Muwale75.712.02559.1N/Aa
Mua-livuleziMarkhamia obtusifolia (Baker) SpragueMsewa5.35.06.35.8a,b
Combretum apiculatum Sond.Kakunguni6.03.015.012.4a,b
Bauhinia petersiana BolleMphandula9.15.236.527.4a,b
Bauhinia thonningii Schum.Msekese13.05.061.6N/Aa
Diplorhynchus condylocarpon (Müll. Arg.) PichonThombozi16.210.484.7N/Aa
Annona senegalensis Pers.Mpoza17.08.083.945.3a,b
Markhamia obtusifolia (Baker) SpragueMsewa20.09.3142.8N/Aa
Pterocarpus rotundifolius (Sond.) DruceM’balitsa22.08.5170.1N/Aa
Albizia versicolor Welw. ex Oliv.Mtangatanga23.210.4214.6N/Aa
Bauhinia petersiana BolleMphandula27.47.9366.6N/Aa
Diplorhynchus condylocarpon (Müll. Arg.) PichonThombozi31.813.0407.3169.5a,b
Acacia galpinni Burtt DavyMgundanjira37.714.0932.3232.6a,b
Pterocarpus rotundifolius (Sond.) DruceM'balitsa42.015.0959.6N/Aa
Brachystegia spiciformis Benth.Chumbe47.115.51916.1349.5a,b
Brachystegia bussei HarmsMtwana61.020.53970.51337.1a,b
Pseudolachnostaylis maprouneifolia PaxMsolo61.015.62994.0N/Aa
Brachystegia spiciformis Benth.Chumbe69.521.04580.61669.5a,b
Brachystegia bussei HarmsMtwana81.722.06758.4N/Aa
TsambaUapaca kirkiana Müll. Arg.Msuku8.46.518.0N/Aa
Brachystegia floribunda Benth.Tsamba9.59.037.5N/Aa
Pseudolachnostaylis maprouneifolia PaxMsolo12.47.0105.9N/Aa
Brachystegia floribunda Benth.Tsamba19.98.0287.6N/Aa
Parinari excelsa SabineMpembu21.010.0205.186.2a,b
Julbernadia globiflora (Benth.) TroupinKachumbe21.010.0223.0N/Aa
Brachystegia spiciformis Benth.Chumbe21.212.0271.7210.7a,b
Uapaca sansibarica PaxMsokolowe22.210.0212.3110.2a,b
Pericorpsis angolensis (Baker) MeeuwenMuwanga28.48.0440.2249.4a,b
Uapaca kirkiana Müll. Arg.Msuku30.311.0581.9277.7a,b
Pterocarpus angolensis DC.Mlombwa31.317.0667.7218.5a,b
Brachystegia utilis Burtt Davy & Hutch.Nzale32.014.0867.6385.8a,b
Uapaca sansibarica PaxMsokolowe32.012.0445.9177.3a,b
Brachystegia floribunda Benth.Tsamba34.512.0683.0N/Aa
Julbernadia globiflora (Benth.) TroupinKachumbe37.518.01014.0N/Aa
Faurea speciosa (A. Rich.) Pic.Serm.Chisese38.716.0693.7N/Aa
Julbernadia globiflora (Benth.) TroupinMchenga67.421.04591.21397.5a,b
Brachystegia spiciformis Benth.Chumbe75.025.05866.21240.0a,b
Note: a = aboveground only; a, b = both above-and belowground; N/A = not applicable.
Table A2. DF-ratio for merchantable stem, branches, twigs, roots, and root crown in the four study sites.
Table A2. DF-ratio for merchantable stem, branches, twigs, roots, and root crown in the four study sites.
SiteAbovegroundBelowground
No. of TreesMerchantable StemBranchesTwigsNo. of TreesRootsRoot crown
MeanMinMaxMeanMinMaxMeanMinMaxMeanMinMaxMeanMinMax
Mtangatanga200.610.480.770.570.440.730.510.300.66120.580.460.690.590.530.67
Kongwe180.560.360.710.540.350.650.520.370.65100.580.480.720.570.390.67
Mua-livulezi180.600.540.690.610.540.670.560.440.6690.600.490.700.590.460.68
Tsamba180.590.450.700.580.450.670.530.380.65100.520.450.630.570.510.61
All740.590.360.770.580.350.730.530.300.66410.570.450.730.580.390.68
Note: The number of sample trees for Kongwe for the twigs section is 16 instead of 18 because two trees did not have twigs.
Table A3. Covariance matrices of all valid above- and belowground models.
Table A3. Covariance matrices of all valid above- and belowground models.
ComponentModel No.ModelVariableCovariance Matrix
Belowground10.284615 × dbh1.992658 interceptdbh
intercept0.01184
dbh−0.010180.00882
Aboveground10.21691 × dbh2.318391 interceptdbh
intercept0.00251
dbh−0.002630.00278
20.103685 × dbh1.921719 × ht0.844561 interceptdbhht
intercept0.00058
dbh−0.000450.00166
ht−0.00129−0.000940.00580
30.290457 × dbh2.283998 × ρ 0.443619 interceptdbh ρ
intercept0.00592
dbh−0.004220.00305
ρ 0.00557−0.003460.01364

References

  1. Chidumayo, E.N.; Gumbo, D.J. The Dry Forests and Woodlands of Africa: Managing for Products and Services; Earthscan: London, UK, 2010. [Google Scholar]
  2. Abbot, P.; Lowore, J.; Werren, M. Models for the estimation of single tree volume in four miombo woodland types. For. Ecol. Manag. 1997, 97, 25–37. [Google Scholar] [CrossRef]
  3. Munishi, P.K.T.; Temu, R.P.C.; Soka, G. Miombo ecosystem in the Lake Rukwa basin, southern Tanzania: Implications for conservation. J. Ecol. Nat. Environ. 2010, 3, 63–71. [Google Scholar]
  4. Ryan, C.M.; Williams, M.; Grace, J. Above- and belowground carbon stocks in a miombo woodland landscape of Mozambique. Biotropica 2011, 43, 423–432. [Google Scholar] [CrossRef]
  5. Walker, S.M.; Desanker, P.V. The impact of land use on soil carbon in miombo woodlands of Malawi. For. Ecol. Manag. 2004, 203, 345–360. [Google Scholar] [CrossRef]
  6. Abdallah, J.M.; Monela, G.G. Overview of miombo woodlands in Tanzania. In Management of Indigenous Tree Species for Ecosystem Restoration and Wood Production in Semi-Arid Miombo Woodlands in Eastern Africa; Metla, Ed.; Working Papers of the Finnish Forest Research Institute: Metla, Finland, 2007; Volume 50, pp. 9–23. [Google Scholar]
  7. Government of Malawi. Forest Resource Mapping Project under the Japanese Grant for the Forest Preservation Programme to the Republic of Malawi; Ministry of Environment and Climate Change Management: Lilongwe, Malawi, 2012.
  8. Government of Malawi. Malawi State of Environment and Outlook Report; Ministry of Natural Resources, Energy and Environment: Lilongwe, Malawi, 2010.
  9. Government of Malawi. Malawi Population Data Sheet; Malawi National Statistics office: Zomba, Malawi, 2012.
  10. FAO (Food and Agriculture Organisation of the United Nations). Global Forest Resources Assessment 2015; FAO: Rome, Italy, 2015. [Google Scholar]
  11. Akindele, S.O.; LeMay, V.M. Development of tree volume equations for common timber species in the tropical rain forest area of Nigeria. For. Ecol. Manag. 2006, 226, 41–48. [Google Scholar] [CrossRef]
  12. West, P.W. Tree and Forest Measurement, 3rd ed.; Springer International Publishing: Cham, Switzerland, 2015. [Google Scholar]
  13. Chave, J.; Andalo, C.; Brown, S.; Cairns, M.A.; Chambers, J.Q.; Eamus, D.; Fölster, H.; Fromard, F.; Higuchi, N.; Kira, T.; et al. Tree allometry and improved estimation of carbon stocks and balance in tropical forests. Oecologia 2005, 145, 87–99. [Google Scholar] [CrossRef] [PubMed]
  14. Cairns, M.A.; Brown, S.; Helmer, E.H.; Baumgardner, G.A. Root biomass allocation in the world’s upland forests. Oecologia 1997, 111, 1–11. [Google Scholar] [CrossRef]
  15. Henry, M.; Picard, N.; Trotta, C.; Manlay, R.; Valentini, R.; Bernoux, M.; Saint-André, L. Estimating tree biomass of sub-Saharan African forests: A review of available allometric equations. Silva Fenn. 2011, 45, 477–569. [Google Scholar] [CrossRef]
  16. Mwakalukwa, E.E.; Meilby, H.; Treue, T. Volume and aboveground biomass models for dry miombo woodland in Tanzania. Int. J. For. Res. 2014, 2014. [Google Scholar] [CrossRef]
  17. Mate, R.; Johansson, T.; Sitoe, A. Biomass equations for tropical forest tree species in Mozambique. Forests 2014, 5, 535–556. [Google Scholar] [CrossRef]
  18. Chidumayo, E.N. Estimating tree biomass and changes in root biomass following clear-cutting of Brachystegia-Julbernardia (miombo) woodland in central Zambia. Environ. Conserv. 2014, 41, 54–63. [Google Scholar] [CrossRef]
  19. Chamshama, S.A.O.; Mugasha, A.G.; Zahabu, E. Stand biomass and volume estimation for miombo woodlands at Kitulangalo, Morogoro, Tanzania. South. Afr. For. J. 2004, 200, 59–70. [Google Scholar] [CrossRef]
  20. Malimbwi, R.E.; Solberg, B.; Luoga, E. Estimation of biomass and volume in miombo woodland at Kitulangalo forest reserve, Tanzania. J. Trop. For. Sci. 1994, 7, 230–242. [Google Scholar]
  21. Mugasha, W.A.; Eid, T.; Bollandsås, O.M.; Malimbwi, R.E.; Chamshama, S.A.O.; Zahabu, E.; Katani, J.Z. Allometric models for prediction of above- and belowground biomass of trees in the miombo woodlands of Tanzania. For. Ecol. Manag. 2013, 310, 87–101. [Google Scholar] [CrossRef]
  22. Giliba, R.A.; Boon, E.K.; Kayombo, C.J.; Musamba, E.B.; Kashindye, A.M.; Shayo, P.F. Species composition, richness and diversity in miombo woodlands of Bereku forest reserve, Tanzania. J. Biodivers. 2011, 2, 1–7. [Google Scholar]
  23. Kalaba, F.K.; Quinn, C.H.; Dougill, A.J.; Vinya, R. Floristic composition, species diversity and carbon storage in charcoal and agriculture fallows and management implications in Miombo woodlands of Zambia. For. Ecol. Manag. 2013, 304, 99–109. [Google Scholar] [CrossRef]
  24. Mwakalukwa, E.E.; Meilby, H.; Treue, T. Floristic composition, structure, and species associations of dry miombo woodland in Tanzania. ISRN Biodivers. 2014, 2014, 153278. [Google Scholar] [CrossRef]
  25. Dewees, P.A.; Campbell, B.M.; Katerere, Y.; Sitoe, A.; Cunningham, A.B.; Angelsen, A.; Wunder, S. Managing the Miombo Woodlands of Southern Africa: Policies, Incentives and Options for the Rural Poor; World Bank, Program on Forests (PROFOR): Washington, DC, USA, 2010; Volume 2, pp. 57–73. [Google Scholar]
  26. Mugasha, W.A.; Bollandsås, O.M.; Eid, T. Relationships between diameter and height of trees in natural tropical forest in Tanzania. South. For. J. For. Sci. 2013, 75, 221–237. [Google Scholar] [CrossRef]
  27. Missanjo, E.; Kamanga-Thole, G.; Mtambo, C.; Chisinga, O. Evaluation of natural regeneration and tree species diversity in miombo woodlands in Malawi. J. Biodivers. Manag. For. 2014, 3. [Google Scholar] [CrossRef]
  28. Brown, S. Measuring carbon in forests: current status and future challenges. Environ. Pollut. 2002, 116, 363–372. [Google Scholar] [CrossRef]
  29. Njana, M.A.; Eid, T.; Zahabu, E.; Malimbwi, R.E. Procedures for quantification of belowground biomass of three mangrove tree species. Wetl. Ecol. Manag. 2015, 23, 749–764. [Google Scholar] [CrossRef]
  30. Intergovernmental panel on climate change (IPCC). Revised Supplementary Methods and Good Practice Guidance Arising from the Kyoto Protocol; IPCC: Geneva, Switzerland, 2014. [Google Scholar]
  31. Chave, J.; Condit, R.; Aguilar, S.; Hernandez, A.; Lao, S.; Perez, R. Error propagation and scaling for tropical forest biomass estimates. Philos. Trans. Royal Soc. Lond. B Biol. Sci. 2004, 359, 409–420. [Google Scholar] [CrossRef] [PubMed]
  32. Clark, D.B.; Kellner, J.R. Tropical forest biomass estimation and the fallacy of misplaced concreteness. J. Veg. Sci. 2012, 23, 1191–1196. [Google Scholar]
  33. Magnussen, S.; Carillo Negrete, O. Model errors in tree biomass estimates computed with an approximation to a missing covariance matrix. Carbon Balance Manag. 2015, 10. [Google Scholar] [CrossRef] [PubMed]
  34. McRoberts, R.E.; Westfall, J.A. Effects of uncertainty in model predictions of individual tree volume on large area volume estimates. For. Sci. 2013, 60, 34–42. [Google Scholar] [CrossRef]
  35. Breidenbach, J.; Anton-Fernandez, C.; Petersson, H.; McRoberts, R.E.; Astrup, R. Quantifying the model-related variability of biomass stock and change estimates in the Norwegian National Forest Inventory. For. Sci. 2014, 60, 25–33. [Google Scholar] [CrossRef]
  36. Hardcastle, P.D. A Preliminary Silvicultural Classification of Malawi; Forestry Research Institute of Malawi: Zomba, Malawi, 1978. [Google Scholar]
  37. Kachamba, D.J.; Eid, T. Total tree, merchantable stem and branch volume models for miombo woodlands of Malawi. South. For. 2016. [Google Scholar] [CrossRef]
  38. Mauya, E.W.; Mugasha, W.A.; Zahabu, E.; Bollandsås, O.M.; Eid, T. Models for estimation of tree volume in the miombo woodlands of Tanzania. South. For. 2014, 76, 209–219. [Google Scholar] [CrossRef]
  39. Chave, J.; Réjou-Méchain, M.; Búrquez, A.; Chidumayo, E.N.; Colgan, M.S.; Delitti, W.B.C.; Duque, A.; Eid, T.; Fearnside, P.M.; Goodman, R.C.; et al. Improved allometric models to estimate the aboveground biomass of tropical trees. Glob. Chang.Biol. 2014, 20, 3177–3190. [Google Scholar] [CrossRef] [PubMed]
  40. Chave, J.; Coomes, D.A.; Jansen, S.; Lewis, S.L.; Swenson, N.G.; Zanne, A.E. Towards a worldwide wood economics spectrum. Ecol. Lett. 2009, 12, 351–366. [Google Scholar] [CrossRef] [PubMed]
  41. Zanne, A.E.; Lopez-Gonzalez, G.; Coomes, D.A.; Ilic, J.; Jansen, S.; Lewis, S.L.; Miller, R.B.; Swenson, N.G.; Wiemann, M.C.; Chave, J. Global Wood Density Database. Available online: http://dx.doi.org/10.5061/dryad.234 (accessed on 15 October 2015).
  42. SAS Institute Inc. SAS 9.4 TS Level 1 M1; SAS Institute Inc.: Cary, NC, USA, 2012. [Google Scholar]
  43. Akaike, H. A new look at the statistical model identification. Inst. Electr. Electron. Eng. IEEE Trans. Autom. Control 1974, 19, 716–723. [Google Scholar] [CrossRef]
  44. James, G.; Witten, D.; Hastie, T.; Tibshirani, R. An Introduction to Statistical Learning with Application in R; Springer: New York, NY, USA, 2013; Volume 103. [Google Scholar]
  45. Mokany, K.; Raison, R.J.; Prokushkin, A.S. Critical analysis of root : Shoot ratios in terrestrial biomes. Glob. Chang. Biol. 2006, 12, 84–96. [Google Scholar] [CrossRef]
  46. Munishi, P.K.T.; Shear, T.H. Carbon storage in afromontane rainforests of the eastern arc mountains of Tanzania: Their net contribution to atmospheric carbon. J. Trop. For. Sci. 2004, 16, 78–93. [Google Scholar]
  47. Gertner, G. Prediction Bias and Response Surface Curvature. For. Sci. 1991, 37, 755–765. [Google Scholar]
  48. Larjavaara, M.; Muller-Landau, H.C. Measuring tree height: A quantitative comparison of two common field methods in a moist tropical forest. Methods Ecol. Evol. 2013, 4, 793–801. [Google Scholar] [CrossRef]
  49. Hunter, M.O.; Keller, M.; Victoria, D.; Morton, D.C. Tree height and tropical forest biomass estimation. Biogeosciences 2013, 10, 8385–8399. [Google Scholar] [CrossRef]

Share and Cite

MDPI and ACS Style

Kachamba, D.J.; Eid, T.; Gobakken, T. Above- and Belowground Biomass Models for Trees in the Miombo Woodlands of Malawi. Forests 2016, 7, 38. https://doi.org/10.3390/f7020038

AMA Style

Kachamba DJ, Eid T, Gobakken T. Above- and Belowground Biomass Models for Trees in the Miombo Woodlands of Malawi. Forests. 2016; 7(2):38. https://doi.org/10.3390/f7020038

Chicago/Turabian Style

Kachamba, Daud J., Tron Eid, and Terje Gobakken. 2016. "Above- and Belowground Biomass Models for Trees in the Miombo Woodlands of Malawi" Forests 7, no. 2: 38. https://doi.org/10.3390/f7020038

Note that from the first issue of 2016, this journal uses article numbers instead of page numbers. See further details here.

Article Metrics

Back to TopTop