Variation of Traits on Seeds and Germination Derived from the Hybridization between the Sections Tacamahaca and Aigeiros of the Genus Populus
Abstract
:1. Introduction
2. Materials and Methods
2.1. Plant Materials
2.2. Pollination Procedure
2.2.1. Water Culture
2.2.2. Pollen Collection and Pollination
2.3. In Vitro Pollen Germination
2.4. Ovary Observation
2.5. Seed Traits Measurements
catkin) ÷ ovule number per capsule × 100%.
2.6. Seedling Traits Measurement
2.7. Statistical Analysis
3. Results
3.1. Variation in Ovary Traits of Female Parents
3.2. Seed Formation Rate and Seed Morphological Variation
3.3. Variation in Seed Traits
3.4. Seed Germination Characteristics
3.5. Variations in Seedling Cotyledon Length and Morphology
4. Discussion and Conclusions
Author Contributions
Funding
Conflicts of Interest
References
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Cultivar | Latin Name | Sexuality | Collection Place |
---|---|---|---|
XY-1 | P. simonii | Female | Manas Plain Forest Farm, Sinkiang |
XY-2 | P. simonii | Female | Tongliao, Inner Mongolia |
XY-3 | P. simonii | Female | Tongliao, Inner Mongolia |
XY-4 | P. simonii | Female | Tongliao, Inner Mongolia |
XY-5 | P. simonii | Male | Tongliao, Inner Mongolia |
XY-6 | P. simonii | Male | Tongliao, Inner Mongolia |
XQ-1 | P. pseudo-simonii | Female | Tongliao, Inner Mongolia |
XQ-2 | P. pseudo-simonii | Female | Tongliao, Inner Mongolia |
XQ-3 | P. pseudo-simonii | Female | Tongliao, Inner Mongolia |
XQ-4 | P. pseudo-simonii | Male | Tongliao, Inner Mongolia |
OH-1 | P. nigra | Male | Tongliao, Inner Mongolia |
OH-2 | P. nigra | Male | Manas Plain Forest Farm, Sinkiang |
ZTY | Populus nigra var. italica | Male | Beijing Botanical Garden |
137 | P. deltoides | Male | Tongliao, Inner Mongolia |
144 | P. deltoides | Male | Tongliao, Inner Mongolia |
154 | P. deltoides | Male | Tongliao, Inner Mongolia |
BJLY3 | Populus deltoides × Populus nigra | Male | Yiling Plain Forest Farm, Sinkiang |
BJY | Populus deltoides × Populus nigra | Male | Yiling Plain Forest Farm, Sinkiang |
DZY | Populus deltoides × Populus nigra | Male | Yiling Plain Forest Farm, Sinkiang |
XMH | Populus simonii × (Populus pyramidalis + Salix matsudana) | Male | Tongliao, Inner Mongolia |
ZY3 | Populus pseudo-simonii × Populus nigra var. lica | Female | Tongliao, Inner Mongolia |
EBY | Populus nigra var. thevestina × Populus simonii | Female | Wuwei, Ganshu |
Evaluated Traits | Sources Variation | |||||||
---|---|---|---|---|---|---|---|---|
Female | Error (Female) | Male | Error (Male) | |||||
df | Mean Squares | df | Mean Squares | df | Mean Squares | df | Mean Squares | |
Days for seed development | 8 | 377.23 ** | 93 | 12.82 | - | - | - | - |
Ovule number of per capsule | 8 | 171.97 ** | 81 | 2.16 | - | - | - | - |
Seed formation rate (%) | 8 | 0.11 ** | 93 | 0.03 | 12 | 0.20 ** | 89 | 0.02 |
Seed length (mm) | 8 | 0 ** | 90 | 0 | 12 | 0 ns | 86 | 0 |
Seed width (mm) | 8 | 0.11 ** | 90 | 0.01 | 12 | 0.06 ** | 86 | 0.02 |
Seed germination rate (%) | 8 | 0.26 ns | 93 | 0.27 | 12 | 1.63 ** | 89 | 0.09 |
Seedling non-radicle rate (%) | 8 | 504.21 ns | 77 | 543.41 | 12 | 1692.17 ** | 73 | 350.27 |
Seedling cotyledon length (mm) | 8 | 2.15 ** | 59 | 0.44 | 9 | 2.28 ** | 59 | 0.39 |
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Zhu, J.; Tian, J.; Wang, J.; Nie, S. Variation of Traits on Seeds and Germination Derived from the Hybridization between the Sections Tacamahaca and Aigeiros of the Genus Populus. Forests 2018, 9, 516. https://doi.org/10.3390/f9090516
Zhu J, Tian J, Wang J, Nie S. Variation of Traits on Seeds and Germination Derived from the Hybridization between the Sections Tacamahaca and Aigeiros of the Genus Populus. Forests. 2018; 9(9):516. https://doi.org/10.3390/f9090516
Chicago/Turabian StyleZhu, Jialei, Ju Tian, Jun Wang, and Shuijing Nie. 2018. "Variation of Traits on Seeds and Germination Derived from the Hybridization between the Sections Tacamahaca and Aigeiros of the Genus Populus" Forests 9, no. 9: 516. https://doi.org/10.3390/f9090516