Wild Boar as a Sylvatic Reservoir of Hepatitis E Virus in Poland: A Cross-Sectional Population Study
Abstract
:1. Introduction
2. Materials and Methods
2.1. Viruses
2.2. Sera, Livers and Faeces of Wild Boar
2.3. Preparation of the HEV RNA Standard
2.4. Detection of Anti-HEV Antibodies in Wild Boars
2.5. Detection of HEV RNA Using Real-Time RT-qPCR
2.6. Statistical Analysis
3. Results
3.1. Prevalence of Anti-HEV Antibodies in the Sera of Wild Boar
3.2. Detection of HEV RNA in Livers and Faeces
4. Discussion
5. Conclusions
Author Contributions
Funding
Acknowledgments
Conflicts of Interest
References
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Regional Directorates of State Forest (RDSF) | Province | Population Size | Number of Animals |
---|---|---|---|
Katowice | Opole, Silesia | 20–30,000 | 43 |
Olsztyn | Warmia-Masuria | 41 | |
Szczecin | Lubuskie, West Pomerania | 53 | |
Szczecinek | Pomerania, West Pomerania | 44 | |
Białystok | Podlaskie, Warmia-Masuria | 10–20,000 | 21 |
Gdańsk | Pomerania, Warmia-Masuria | 22 | |
Krosno | Podkarpackie | 17 | |
Lublin | Lublin, Podkarpackie | 37 | |
Łódź | Łódź, Mazovia | 17 | |
Piła | Wielkopolska | 23 | |
Poznań | Wielkopolska | 38 | |
Radom | Mazovia, Świętokrzyskie | 9 | |
Toruń | Kujawy-Pomerania | 30 | |
Wrocław | Lower Silesia | 25 | |
Zielona Góra | Lubuskie | 25 | |
Kraków | Małopolska | <10,000 | 10 |
Warszawa | Mazovia | 15 | |
Total | 470 |
RDSF | No. of Sample | ELISA (%; 95%CI) | Liver (%; 95%CI) | No. of Sample | Faeces (%; 95%CI) | |||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Positive | Negative | Positive | Negative | Positive | Negative | |||||||||
Białystok | 21 * | 11 | (52.4; 29.8–74.3) | 9 | (42.9; 21.8–66.0) | 2 | (9.5; 1.2–30.4) | 19 | (90.5; 69.6–98.8) | 21 | 0 | (0; 0–16.1) | 21 | (0; 83.8–100) |
Gdańsk | 22 | 11 | (50.0; 28.2–71.8) | 11 | (50.0; 28.2–71.8) | 4 | (18.2; 5.2–40.3) | 18 | (81.1; 59.7–94.8) | 22 | 0 | (0; 0–15.4) | 22 | (0; 84.5–100) |
Katowice | 43 | 23 | (53.5; 37.6–68.8) | 20 | (46.5; 31.2–62.3) | 11 | (25.6; 13.5–41.2) | 32 | (74.4; 58.8–86.5) | 40 | 1 | (2.5; 0–13.1) | 39 | (97.5; 86.8–99.9) |
Kraków | 10 | 1 | (10.0; 0.2–44.5) | 9 | (90.0; 55.5–99.7) | 0 | (0; 0–30.8) | 10 | (100; 69.1–100) | 10 | 0 | (0; 0–30.8) | 10 | (0; 69.1–100) |
Krosno | 17 | 5 | (29.4; 10.3–56) | 12 | (70.6; 44.0–89.7) | 1 | (5.9; 0.2–28.7) | 16 | (94.1; 71.3–99.8) | 16 | 2 | (12.5; 1.5–38.3) | 14 | (87.5; 61.6–98.4) |
Lublin | 37 * | 10 | (27.0; 13.8–44.1) | 24 | (64.9; 47.5–79.8) | 1 | (2.7; 0.1–14.2) | 36 | (97.3; 85.8–99.9) | 35 | 0 | (0; 0–10) | 35 | (0; 89.9–100) |
Łódź | 17 | 6 | (35.3; 14.2–61.7) | 11 | (64.7; 38.3–85.8) | 1 | (5.9; 0.2–28.7) | 16 | (94.1; 71.3–99.8) | 16 | 1 | (6.2; 0.1–30.2) | 15 | (93.7; 69.7–99.8) |
Olsztyn | 41 * | 23 | (56.1; 39.7–71.5) | 17 | (41.5; 26.3–57.9) | 8 | (19.5; 8.8–34.9) | 33 | (80.5; 65.1–91.2) | 38 | 4 | (10.5; 2.9–24.8) | 34 | (89.4; 75.1–97) |
Piła | 23 * | 15 | (65.2; 42.7–83.6) | 7 | (30.4; 13.2–52.9) | 3 | (13; 2.8–33.6) | 20 | (87; 66.4–97.2) | 22 | 2 | (9.1; 1.1–29.1) | 20 | (90.9; 70.8–98.8) |
Poznań | 38 | 18 | (47.4; 31–64.2) | 20 | (52.6; 35.8–69.0) | 5 | (13.2; 4.4–28.1) | 33 | (86.8; 71.9–95.6) | 30 | 1 | (3.3; 0–17.2) | 29 | (96.6; 82.7–99.9) |
Radom | 9 | 6 | (66.7; 29.9–92.5) | 3 | (33.3; 7.5–70.1) | 4 | (44.4; 13.7–78.8) | 5 | (55.6; 21.2–86.3) | 9 | 3 | (33.3; 7.4–70) | 6 | (66.6; 29.9–92.5) |
Szczecin | 53 * | 27 | (50.9; 36.8–64.9) | 23 | (43.4; 29.8–57.7) | 8 | (15.1; 6.7–27.6) | 45 | (84.9; 72.4–93.3) | 45 | 2 | (4.4; 0.5–15.1) | 43 | (95.5; 84.5–99.4) |
Szczecinek | 44 * | 22 | (50.0; 34.6–65.4) | 21 | (47.7; 32.5–63.3) | 3 | (6.8; 1.4–18.7) | 41 | (93.2; 81.3–98.6) | 40 | 1 | (2.5; 0–13.1) | 39 | (97.5; 86.8–99.9) |
Toruń | 30 * | 16 | (53.3; 34.3–71.7) | 12 | (40.0; 22.7–59.4) | 5 | (16.7; 5.6–34.7) | 25 | (83.3; 65.3–94.4) | 27 | 5 | (18.5; 6.3–38) | 22 | (81.4; 61.9–93.7) |
Warszawa | 15 * | 7 | (46.7; 21.3–73.4) | 7 | (46.7; 21.3–73.4) | 0 | (0; 0–21.8) | 15 | (100; 78.2–100) | 15 | 0 | (0; 0–21.8) | 15 | (0; 78.1–100) |
Wrocław | 25 * | 13 | (52.0; 31.3–72.2) | 11 | (44.0; 24.4–65.1) | 0 | (0; 0–13.7) | 25 | (100; 86.3–100) | 25 | 3 | (12.5; 2.6–32.3) | 21 | (87.5; 67.6–97.3) |
Zielona Góra | 25 | 18 | (72.0; 50.6–87.9) | 7 | (28.0; 12.1–49.4) | 1 | (4; 0.1–20.3) | 24 | (96; 79.7–99.9) | 23 | 2 | (8.7; 1–28) | 21 | (91.3; 71.9–98.9) |
Total (%; 95%CI) | 470 (100) | 232 (49.4; 44.7–54) | 224 (47.7; 43.1–52.3) | 57 (12.1; 9.3–15.4) | 413 (87.9; 84.6–90.7) | 433 (100) | 27 (6.2; 4.1–8.9) | 406 (91.3; 91–93.7) |
Number of Animals * | ELISA Results | Real-Time (RT)-qPCR Results | |
---|---|---|---|
Serum | Liver (G.C./g) | Faeces (G.C./mL) | |
16 | + | 1.6 × 103–9.4 × 109 | 1.2 × 103–9.3 × 107 |
31 (1) | + | 8.9–1.7 × 1011 | - |
5 | + | - | 38–1.7 × 105 |
180 (8) | + | - | - |
4 | − | 3.7–7.1 × 108 | 8 × 102–3.1 × 106 |
11 (3) | − | 1.4–1.1 × 109 | - |
2 | − | - | 42–3.9 × 104 |
207 (27) | − | - | - |
14 | +/− | - | - |
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Kozyra, I.; Jabłoński, A.; Bigoraj, E.; Rzeżutka, A. Wild Boar as a Sylvatic Reservoir of Hepatitis E Virus in Poland: A Cross-Sectional Population Study. Viruses 2020, 12, 1113. https://doi.org/10.3390/v12101113
Kozyra I, Jabłoński A, Bigoraj E, Rzeżutka A. Wild Boar as a Sylvatic Reservoir of Hepatitis E Virus in Poland: A Cross-Sectional Population Study. Viruses. 2020; 12(10):1113. https://doi.org/10.3390/v12101113
Chicago/Turabian StyleKozyra, Iwona, Artur Jabłoński, Ewelina Bigoraj, and Artur Rzeżutka. 2020. "Wild Boar as a Sylvatic Reservoir of Hepatitis E Virus in Poland: A Cross-Sectional Population Study" Viruses 12, no. 10: 1113. https://doi.org/10.3390/v12101113