Suppression of nbe-miR1919c-5p Expression in Nicotiana benthamiana Enhances Tobacco Curly Shoot Virus and Its Betasatellite Co-Infection
Abstract
:1. Introduction
2. Materials and Methods
2.1. Gene Cloning and Vector Construction
2.2. Plant Growth, Virus Inoculation and Agro-infiltration
2.3. DNA Extraction and Virus DNA Accumulation Analysis
2.4. RNA Extraction and Quantitative Reverse Transcription Polymerase Chain Reaction (qRT-PCR)
2.5. Prediction and Quantification of Target Genes
3. Results
3.1. Leaf Curling Symptoms Induced by TbCSV/TbCSB Co-Infection in N. benthamiana
3.2. Suppression of Nbe-miR1919c-5p Expression Enhances Leaf Curling Symptoms Caused by TbCSV/TbCSB Co-Infection
3.3. Suppression of Nbe-miR1919c-5p Expression Enhances TbCSV and TbCSB DNA Accumulation in N. benthamiana Plants
3.4. Over-Expression of Nbe-miR1919c-5p Reduced TbCSV and TbCSB DNA Accumulation in N. benthamiana Plants
3.5. Quantification of Nbe-miR1919c-5p Target Gene Expression
4. Discussion
5. Conclusions
Supplementary Materials
Author Contributions
Funding
Conflicts of Interest
References
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miRNA | Target Gene | Expectation | UPE | miRNA Start | miRNA End | Target Start | Target End | miRNA Aligned Fragment | Target Aligned Fragment | Inhibition | Multiplicity |
---|---|---|---|---|---|---|---|---|---|---|---|
Nbe-miR1919c-5p | Niben101Scf04663g00007 | 2.0 | 15.475 | 1 | 21 | 254 | 274 | UGUCGCAGAUGACUUUCGCCC | AUGCGAAAGUCAUCUGCGACA | Cleavage | 1 |
Nbe-miR1919c-5p | Niben101Scf02655g01001 | 2.0 | 16.74 | 1 | 21 | 485 | 505 | UGUCGCAGAUGACUUUCGCCC | AUGCGAAAGUCAUCUGCGACA | Cleavage | 1 |
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Du, J.; Wu, R.; Liu, Z.; Sun, M.; Ghanem, H.; Li, M.; Wu, G.; Qing, L. Suppression of nbe-miR1919c-5p Expression in Nicotiana benthamiana Enhances Tobacco Curly Shoot Virus and Its Betasatellite Co-Infection. Viruses 2020, 12, 392. https://doi.org/10.3390/v12040392
Du J, Wu R, Liu Z, Sun M, Ghanem H, Li M, Wu G, Qing L. Suppression of nbe-miR1919c-5p Expression in Nicotiana benthamiana Enhances Tobacco Curly Shoot Virus and Its Betasatellite Co-Infection. Viruses. 2020; 12(4):392. https://doi.org/10.3390/v12040392
Chicago/Turabian StyleDu, Jiang, Rui Wu, Zhuoying Liu, Miao Sun, Hussein Ghanem, Mingjun Li, Gentu Wu, and Ling Qing. 2020. "Suppression of nbe-miR1919c-5p Expression in Nicotiana benthamiana Enhances Tobacco Curly Shoot Virus and Its Betasatellite Co-Infection" Viruses 12, no. 4: 392. https://doi.org/10.3390/v12040392
APA StyleDu, J., Wu, R., Liu, Z., Sun, M., Ghanem, H., Li, M., Wu, G., & Qing, L. (2020). Suppression of nbe-miR1919c-5p Expression in Nicotiana benthamiana Enhances Tobacco Curly Shoot Virus and Its Betasatellite Co-Infection. Viruses, 12(4), 392. https://doi.org/10.3390/v12040392