Mutation of Hydrophobic Residues in the C-Terminal Domain of the Marburg Virus Matrix Protein VP40 Disrupts Trafficking to the Plasma Membrane
Abstract
:1. Introduction
2. Materials and Methods
2.1. Site Directed Mutagenesis
2.2. Cell Culture
2.3. Heterologous Expression Test
2.4. Confocal Imaging and Analysis
2.5. Transmission Electron Microscopy
2.6. Protein Expression and Purification
2.7. Multilamellar Vesicle Sedimentation Assay
2.8. Sedimentation Velocity Measurements
2.9. Molecular Dynamics Simulations
3. Results
3.1. A Hydrophobic Loop in the VP40 CTD is Conserved Across eVP40 and mVP40
3.2. Cellular Imaging of mVP40 Hydrophobic Mutants Reveals a Stalled Vesicle Morphology
3.3. Transmission Electron Microscopy
3.4. Assessment of WT mVP40 Dimer Structure and Lipid Binding Properties with Mutation of the CTD Hydrophobic Patch
3.5. Investigation of WT mVP40 Co-Expression with mVP40 Hydrophobic Mutations
3.6. Molecular Dynamics Simulations of mVP40 and eVP40
4. Discussion
5. Conclusions
Supplementary Materials
Author Contributions
Funding
Acknowledgments
Conflicts of Interest
References
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Wijesinghe, K.J.; McVeigh, L.; Husby, M.L.; Bhattarai, N.; Ma, J.; Gerstman, B.S.; Chapagain, P.P.; Stahelin, R.V. Mutation of Hydrophobic Residues in the C-Terminal Domain of the Marburg Virus Matrix Protein VP40 Disrupts Trafficking to the Plasma Membrane. Viruses 2020, 12, 482. https://doi.org/10.3390/v12040482
Wijesinghe KJ, McVeigh L, Husby ML, Bhattarai N, Ma J, Gerstman BS, Chapagain PP, Stahelin RV. Mutation of Hydrophobic Residues in the C-Terminal Domain of the Marburg Virus Matrix Protein VP40 Disrupts Trafficking to the Plasma Membrane. Viruses. 2020; 12(4):482. https://doi.org/10.3390/v12040482
Chicago/Turabian StyleWijesinghe, Kaveesha J., Luke McVeigh, Monica L. Husby, Nisha Bhattarai, Jia Ma, Bernard S. Gerstman, Prem P. Chapagain, and Robert V. Stahelin. 2020. "Mutation of Hydrophobic Residues in the C-Terminal Domain of the Marburg Virus Matrix Protein VP40 Disrupts Trafficking to the Plasma Membrane" Viruses 12, no. 4: 482. https://doi.org/10.3390/v12040482