Semipersistently Transmitted, Phloem Limited Plant Viruses Are Inoculated during the First Subphase of Intracellular Stylet Penetrations in Phloem Cells
Round 1
Reviewer 1 Report
The authors have generated novel information on the transmission of BPV, a Closterovirus, and shown the key phases of aphid feeding involved in its transmission. This is highly important as the Closertovirus (and related criniviruses) are important pathogens of many crops worldwide and the mechanisms of their transmission remain poorly defined. Here the key role of phase II-1 is demonstrated and contrasts significantly to the mechanisms of transmission of CaMV, suggesting differences in their sites of binding or transmission may differ. This adds significantly to the knowledge to aphid-virus transmission and its' acceptance is recommended. Some improvements to the language could be made to clarify the manuscript and these are outlined below.
Line 34: change 'associated to virus transmission' to 'associated with virus transmission'
Line 77: change to 'was used in the experiments'
Line 79: change to 'maintained on'
Line 297: change to 'host plant from the'
Line 305: change to 'since we manually disturbed the feeding'
Line 429: Reference 17 and 18 are a duplicate.
Author Response
Comments to reviewers
Authors are grateful for the positive feedback from the reviewers. The suggested modifications detailed below have been now included in the last version of the manuscript.
Reviewer 1
General comments:
The authors have generated novel information on the transmission of BPV, a Closterovirus, and shown the key phases of aphid feeding involved in its transmission. This is highly important as the Closterovirus (and related criniviruses) are important pathogens of many crops worldwide and the mechanisms of their transmission remain poorly defined. Here the key role of phase II-1 is demonstrated and contrasts significantly to the mechanisms of transmission of CaMV, suggesting differences in their sites of binding or transmission may differ. This adds significantly to the knowledge to aphid-virus transmission and its' acceptance is recommended. Some improvements to the language could be made to clarify the manuscript and these are outlined below.
Minor comments:
Line 34: change 'associated to virus transmission' to 'associated with virus transmission'
Comment amended in the text (Line 34).
Line 77: change to 'was used in the experiments'
Comment amended in the text (Line 85).
Line 79: change to 'maintained on'
Comment amended in the text (Line 87).
Line 297: change to 'host plant from the'
Comment amended in the text (Line 322).
Line 305: change to 'since we manually disturbed the feeding'
Comment amended in the text (Line 329).
Line 429: Reference 17 and 18 are a duplicate.
Reference 18 was actually the DOI for that precise reference. References’ numbers moved in the list from their original order when adapting the manuscript to journal format. References are now indicated accordingly in the reference list.
Reviewer 2 Report
This paper describes the Beet yellows virus transmission by an aphid vector whose feeding was monitoted by the EPG technique. The manuscript is well written and may be published after a minor revision.
L 61-70 – The description of the current data on the BYV transmission are too much in passing. A brief description of the virion structure and mentioning that the minor CP, HSP70h, and p64 are involved in aphid transmission (Killiny et al. 2016 Appl Environ Biol 82:6294-6302) are in order here.
The References and their quotation in the text do not seem to correspond to each other, and a careful checking is necessary.
L 68 – the reference 19 (Virology 1994, 198, 311-324.) does not refer to luteoviruses.
L 208 – the reference 21 (Mol. Plant Pathol. 2003, 4, 91-98.) does not mention the SPSS 25.0.0.1 package.
Some references in the list are not mentioned in the text.
Author Response
Reviewer 2
General comments:
This paper describes the Beet yellows virus transmission by an aphid vector whose feeding was monitored by the EPG technique. The manuscript is well written and may be published after a minor revision.
Minor comments:
- L 61-70 – The description of the current data on the BYV transmission are too much in passing. A brief description of the virion structure and mentioning that the minor CP, HSP70h, and p64 are involved in aphid transmission (Killiny et al. 2016 Appl Environ Biol82:6294-6302) are in order here.
In order to provide a more and concise information in regard with transmission of BYV, the following sentences were included as suggested, further including a new reference (Lines 60-67) as follows:
‘’The transmission of the whitefly-transmitted Lettuce infectious yellows virus (LIYV, Closterovirus) is determined by a minor coat protein (CPm) retention mechanism in the anterior foregut of its vector [16]. Also, Citrus tristeza virus (CTV, Closterovirus) has been suggested to bind to the N-acetylglucosamine (NAG) moieties of the cuticular surface of its vector Toxoptera citricida via the CPm, in addition to p61 and p65 heat shock proteins [17]. In case of BYV, efficient assembly by CPm requires homologous Hsp70h, p64 and p20 proteins, with these proteins also likely playing a role in virus transmission in addition to the CPm [18].’’
- The References and their quotation in the text do not seem to correspond to each other, and a careful checking is necessary.
References’ numbers moved in the list when adapting it to journal format. References are now indicated accordingly in both the text and along the references list.
- L 68 – the reference 19 (Virology 1994, 198, 311-324.) does not refer to luteoviruses.
References are now indicated accordingly.. L 208 – the reference 21 (Mol. Plant Pathol. 2003, 4, 91-98.) does not mention the SPSS 25.0.0.1 package.
References are now indicated accordingly.- Some references in the list are not mentioned in the text.
All references in the refences list were mentioned in the text. However, the format modification led into greater number of references in the list. This issue has been amended and all references are now expressed accordingly in both the text and the references list.
