Genetic and Pathogenic Characterization of QX(GI-19)-Recombinant Infectious Bronchitis Viruses in South Korea
Abstract
:1. Introduction
2. Materials and Methods
2.1. Viruses
2.2. Viral RNA Extraction, RT-PCR, and Sequencing
2.3. Phylogenetic Analysis, Sequence Comparisons, and Recombination Analysis
2.4. Pathogenicity Testing
2.5. Statistical Analysis
3. Results
3.1. Phylogenetic Analysis
3.2. Sequence Comparisons
3.3. Recombination Analysis
3.4. Animal Experiments and Histopathology
4. Discussion
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Conflicts of Interest
References
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IBV Strain | Year of Isolation | Type of Chicken | Location | Accession Number |
---|---|---|---|---|
Kr/D342/03 | 2003 | Broiler | Chungcheong | MW984619 |
Kr/D343/03 | 2003 | Breeding | Chungcheong | MW984620 |
Kr/D379/03 | 2003 | Broiler | Chungcheong | MW984621 |
Kr/Q042/05 | 2005 | Broiler | Jeolla | MW984622 |
Kr/D075/05 | 2005 | Broiler | Chungcheong | MW984623 |
Kr/D079/05 | 2005 | Native | Geyonggi | MW984624 |
Kr/D062/06 | 2006 | Broiler | Gangwon | MW984625 |
Kr/Q043/06 | 2006 | Broiler | Geyonggi | MW984626 |
Kr/Q118/08 | 2008 | Broiler | Chungcheong | MW984627 |
Kr/D027/12 | 2012 | Broiler | Chungcheong | MW984628 |
Kr/D021/13 | 2013 | Broiler | Jeolla | MW984629 |
Kr/D022/13 | 2013 | Broiler | Jeolla | MW984630 |
Kr/D024/13 | 2013 | Broiler | Jeolla | MW984631 |
Kr/D025/13 | 2013 | Broiler | Jeolla | MW984632 |
Kr/Q022/13 | 2013 | Broiler | Jeolla | MW984633 |
Kr/Q028/13 | 2013 | Broiler | Jeolla | MW984634 |
Kr/Q030/13 | 2013 | Broiler | Jeolla | MW984635 |
Kr/D068/14 | 2014 | Native | Chungcheong | MW984636 |
Kr/D069/14 | 2014 | Native | Chungcheong | MW984637 |
Kr/D062/16 | 2016 | Native | Geyonggi | MW984638 |
Kr/D053/17 | 2017 | Broiler | Gyeonsang | MW984639 |
Kr/R024/17 | 2017 | Native | Chungcheong | MW984640 |
Kr/Q037/18 | 2018 | Native | Gyeonsang | MW984641 |
Kr/D013/18 | 2018 | Broiler | Jeolla | MW984642 |
Kr/D014/18 | 2018 | Broiler | Geyonggi | MW984643 |
Kr/R030/18 | 2018 | Broiler | Chungcheong | MW984644 |
Kr/R38A/18 | 2018 | Native | Geyonggi | MW984645 |
Kr/D024/18 | 2018 | Layer | Geyonggi | MW984646 |
Kr/D025/18 | 2018 | Broiler | Jeolla | MW984647 |
Kr/Q041/18 | 2018 | Broiler | Gangwon | MW984648 |
Kr/D030/18 | 2018 | Broiler | Geyonggi | MW984649 |
Kr/D038/18 | 2018 | Layer | Chungcheong | MW984650 |
Kr/R038/18 | 2018 | Native | Geyonggi | MW984651 |
Kr/R109/18 | 2018 | Native | Gyeonsang | MW984652 |
Kr/Q054/18 | 2018 | Broiler | Jeolla | MW984653 |
Kr/Q057/18 | 2018 | Broiler | Jeolla | MW984654 |
Kr/D048/18 | 2018 | Broiler | Chungcheong | MW984655 |
Kr/D063/18 | 2018 | Broiler | Geyonggi | MW984656 |
Kr/R084/18 | 2018 | Broiler | Chungcheong | MW984657 |
Kr/Q063/18 | 2019 | Broiler | Chungcheong | MW984658 |
Kr/D005/19 | 2019 | Layer | Gyeonsang | MW984659 |
Kr/Q002/19 | 2019 | Broiler | Chungcheong | MW984660 |
Kr/Q008/19 | 2019 | Broiler | Gyeonsang | MW984661 |
Kr/D021/19 | 2019 | Broiler | Jeolla | MW984662 |
Kr/R019/19 | 2019 | Broiler | Gyeonsang | MW984663 |
Kr/R024/19 | 2019 | Broiler | Gyeonsang | MW984664 |
Kr/R014/19 | 2019 | Broiler | Gyeonsang | MW984665 |
Kr/Q010/19 | 2019 | Broiler | Gyeonsang | MW984666 |
Kr/D030/19 | 2019 | Broiler | Gyeonsang | MW984667 |
Kr/D031/19 | 2019 | Layer | Geyonggi | MW984668 |
Kr/D034/19 | 2019 | Broiler | Jeolla | MW984669 |
Kr/D038/19 | 2019 | Broiler | Jeolla | MW984670 |
Kr/D039/19 | 2019 | Broiler | Geyonggi | MW984671 |
Kr/D060/19 | 2019 | Native | Chungcheong | MW984672 |
Kr/D068/19 | 2019 | Layer | Geyonggi | MW984673 |
Kr/D072/19 | 2019 | Broiler | Chungcheong | MW984674 |
Isolate | Recombinant Pattern | Ciliostasis Score A | |||
---|---|---|---|---|---|
3 dpi | 7 dpi | 14 dpi | 21 dpi | ||
Kr/D030/18 | rGI-19-II | 0.0 ± 0.0 B | 4.0 ± 0.0 | 2.3 ± 0.6 | 0.9 ± 0.6 |
Kr/Q037/18 | rGI-19-III | 0.0 ± 0.1 | 2.1 ± 0.9 | 3.9 ± 0.2 | 2.6 ± 0.9 |
Kr/D025/18 | GI-19 | 0.0 ± 0.0 | 3.7 ± 0.5 | 4.0 ± 0.0 | 0.2 ± 0.5 |
Control | 0.0 ± 0.0 | 0.0 ± 0.0 | 0.0 ± 0.0 | 0.0 ± 0.0 |
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Youn, S.-Y.; Lee, J.-Y.; Bae, Y.-C.; Kwon, Y.-K.; Kim, H.-R. Genetic and Pathogenic Characterization of QX(GI-19)-Recombinant Infectious Bronchitis Viruses in South Korea. Viruses 2021, 13, 1163. https://doi.org/10.3390/v13061163
Youn S-Y, Lee J-Y, Bae Y-C, Kwon Y-K, Kim H-R. Genetic and Pathogenic Characterization of QX(GI-19)-Recombinant Infectious Bronchitis Viruses in South Korea. Viruses. 2021; 13(6):1163. https://doi.org/10.3390/v13061163
Chicago/Turabian StyleYoun, So-Youn, Ji-Youn Lee, You-Chan Bae, Yong-Kuk Kwon, and Hye-Ryoung Kim. 2021. "Genetic and Pathogenic Characterization of QX(GI-19)-Recombinant Infectious Bronchitis Viruses in South Korea" Viruses 13, no. 6: 1163. https://doi.org/10.3390/v13061163