Pathological Study of Demyelination with Cellular Reactions in the Cerebellum of Dogs Infected with Canine Distemper Virus
Abstract
:1. Introduction
2. Materials and Methods
2.1. Dogs
2.2. Histopathology and Immunohistochemistry
2.3. Image Capture and Analysis
2.4. Statistical Analysis
3. Results
3.1. Relationship Between Each Stage and Age or Sex
3.2. Histopathological and Immunohistochemical Findings
4. Discussion
5. Conclusions
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Acknowledgments
Conflicts of Interest
References
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Primary Antibody | Source | Dilution | Species/Type | Detection System |
---|---|---|---|---|
MCA 1893 Canine Distemper Virus | Biorad | 1:250 | Mouse/monoclonal | Mouse-on-canine HRP-Polymer |
Glial Fibrillary Protein (GFAP) | Biocare Medical | RTU * | Mouse/monoclonal | MACH 4 Universal HRP-Polymer |
Anti-Iba1 (ab5076) | Abcam | 1:1000 | Goat/polyclonal | Rabbit Anti-Goat IgG H&L (HRP) ab6741 |
Calbindin (orb5912) | Biorbyt | 1:200 | Rabbit/polyclonal | MACH 4 Universal HRP-Polymer |
Groups | Number of Animals | Average Month Age (Range) | Histopathological Findings |
---|---|---|---|
Control | 5 | (45 days old–6 years old) | No changes |
Acute | 4 | (25 days old–3 years old) | glial cell reaction, inclusion bodies, CDV-immunoreactive particles, meningitis |
Subacute 1 | 4 | (30 days old–3 years old) | demyelination, glial cell reaction, inclusion bodies, CDV-immunoreactive particles, meningitis |
Subacute 2 | 9 | (2 months old–10 years old) | demyelination, glial cell reaction, inclusion bodies, CDV-immunoreactive particles, meningitis, mild decreased granular layer cells, and Purkinje cells |
Subacute 3 | 5 | (3 months old–3 years old) | demyelination, glial cell reaction, inclusion bodies, CDV-immunoreactive particles, meningitis, moderate decreased granular layer cells, and Purkinje cells |
Chronic | 6 | (3 months old–3 years old) | demyelination, gliosis, glial cell reaction, inclusion bodies, CDV-immunoreactive particles, meningitis, severe decreased granular layer cells, and Purkinje cells |
Groups | Number of Animals | Average Month Age (Range) | Numbers | ||
---|---|---|---|---|---|
Astrocytes | Microglia | Purkinje Cells | |||
Control | 5 | (45 days old–6 years old) | 35 ± 11 a | 64 ± 19 | 49 ± 6 a |
Acute | 4 | (25 days old–3 years old) | 41 ± 12 | 64 ± 13 | 42 ± 17 |
Subacute 1 | 4 | (30 days old–3 years old) | 42 ± 13 | 56 ± 16 | 25 ± 6 |
Subacute 2 | 9 | (2 months old–10 years old) | 36 ± 13 | 75 ± 26 | 24 ± 14 b |
Subacute 3 | 5 | (3 months old–3 years old) | 32 ± 12 | 63 ± 26 | 15 ± 12 c |
Chronic | 6 | (3 months old–3 years old) | 63 ± 22 b | 44 ± 17 | 23 ± 13 d |
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Verdes, J.M.; Larrañaga, C.; Godiño, G.; Varela, B.; Yozzi, V.; Iribarnegaray, V.; Delucchi, L.; Yamasaki, K. Pathological Study of Demyelination with Cellular Reactions in the Cerebellum of Dogs Infected with Canine Distemper Virus. Viruses 2024, 16, 1719. https://doi.org/10.3390/v16111719
Verdes JM, Larrañaga C, Godiño G, Varela B, Yozzi V, Iribarnegaray V, Delucchi L, Yamasaki K. Pathological Study of Demyelination with Cellular Reactions in the Cerebellum of Dogs Infected with Canine Distemper Virus. Viruses. 2024; 16(11):1719. https://doi.org/10.3390/v16111719
Chicago/Turabian StyleVerdes, José Manuel, Camila Larrañaga, Guillermo Godiño, Belén Varela, Victoria Yozzi, Victoria Iribarnegaray, Luis Delucchi, and Kanji Yamasaki. 2024. "Pathological Study of Demyelination with Cellular Reactions in the Cerebellum of Dogs Infected with Canine Distemper Virus" Viruses 16, no. 11: 1719. https://doi.org/10.3390/v16111719
APA StyleVerdes, J. M., Larrañaga, C., Godiño, G., Varela, B., Yozzi, V., Iribarnegaray, V., Delucchi, L., & Yamasaki, K. (2024). Pathological Study of Demyelination with Cellular Reactions in the Cerebellum of Dogs Infected with Canine Distemper Virus. Viruses, 16(11), 1719. https://doi.org/10.3390/v16111719