Next Article in Journal
African Swine Fever in Saxony—Disease Dynamics
Previous Article in Journal
Mapping the HPV Landscape in South African Women: A Systematic Review and Meta-Analysis of Viral Genotypes, Microbiota, and Immune Signals
 
 
Search for Articles:
Title / Keyword
Author / Affiliation / Email
Journal
Article Type
 
 
Advanced Search
 
Section
Special Issue
Volume
Issue
Number
Page
 
Journals
Viruses
Volume 16
Issue 12
10.3390/v16121895
viruses-logo
Submit to this Journal Review for this Journal Propose a Special Issue
► Article Menu

Article Menu

share Share announcement Help format_quote Cite question_answer Discuss in SciProfiles
first_page
settings
Order Article Reprints
Font Type:
Arial Georgia Verdana
Font Size:
Aa Aa Aa
Line Spacing:
Column Width:
Background:
Article

Peripheral B Lymphocyte Serves as a Reservoir for the Persistently Covert Infection of Mandarin Fish Siniperca chuatsi Ranavirus

by
Wenfeng Zhang
1,2,3,
Hui Gong
4,
Qianqian Sun
1,2,3,
Yuting Fu
1,2,3,
Xiaosi Wu
1,2,3,
Hengwei Deng
5,
Shaoping Weng
1,2,3,
Jianguo He
1,2,3 and
Chuanfu Dong
1,2,3,*
1
State Key Laboratory of Biocontrol, School of Life Sciences, Sun Yat-sen University, Guangzhou 510275, China
2
Southern Marine Science and Engineering Guangdong Laboratory (Zhuhai), Zhuhai 519000, China
3
Guangdong Province Key Laboratory of Aquatic Economic Animals, Institute of Aquatic Economic Animals, Sun Yat-sen University, Guangzhou 510275, China
4
Fish Diseases Unit, Institute of Biotechnology, Fujian Academy of Agricultural Sciences, Fuzhou 350003, China
5
School of Marine Biology and Fisheries, Hainan University, Haikou 570228, China
*
Author to whom correspondence should be addressed.
Viruses 2024, 16(12), 1895; https://doi.org/10.3390/v16121895
Submission received: 21 November 2024 / Revised: 5 December 2024 / Accepted: 6 December 2024 / Published: 9 December 2024
(This article belongs to the Section Animal Viruses)
Browse Figures
Versions Notes

Abstract

Mandarin fish ranavirus (MRV) is a distinctive member among the genus Ranavirus of the family Iridoviridae. The persistently covert infection of MRV was previously observed in a natural outbreak of MRV, but the underlying mechanism remains unclear. Here, we show that mandarin fish peripheral B lymphocytes are implemented as viral reservoirs to maintain the persistent infection. When mandarin fish were infected with a sublethal dosage of MRV under a nonpermissive temperature (19 °C) and a permissive temperature (26 °C), all of the fish in the 19 °C group survived and entered the persistent phase of infection, characterized by a very low viral load in white blood cells, whereas some of the fish died of MRV infection in the 26 °C group, and the survival fish then initiated a persistent infection status. Raising the temperature, vaccination and dexamethasone treatment can reactivate the quiescent MRV to replicate and result in partial mortality. The viral reservoir investigation showed that IgM+-labeled B lymphocytes, but not CD3Δ+-labeled T lymphocytes and MRC-1+-labeled macrophages, are target cells for the persistent infection of MRV. Moreover, the reactivation of the quiescent MRV was confirmed through a non-TLR5 signal pathway manner. Collectively, we demonstrate the presence of the B cell-dependent persistent infection of ranavirus, and provide a new clue for better understanding the complex infection mechanism of vertebrate iridovirus.
Keywords: mandarin fish ranavirus (MRV); persistent infection; B lymphocytes; reactivation; TLR mandarin fish ranavirus (MRV); persistent infection; B lymphocytes; reactivation; TLR

Share and Cite

MDPI and ACS Style

Zhang, W.; Gong, H.; Sun, Q.; Fu, Y.; Wu, X.; Deng, H.; Weng, S.; He, J.; Dong, C. Peripheral B Lymphocyte Serves as a Reservoir for the Persistently Covert Infection of Mandarin Fish Siniperca chuatsi Ranavirus. Viruses 2024, 16, 1895. https://doi.org/10.3390/v16121895

AMA Style

Zhang W, Gong H, Sun Q, Fu Y, Wu X, Deng H, Weng S, He J, Dong C. Peripheral B Lymphocyte Serves as a Reservoir for the Persistently Covert Infection of Mandarin Fish Siniperca chuatsi Ranavirus. Viruses. 2024; 16(12):1895. https://doi.org/10.3390/v16121895

Chicago/Turabian Style

Zhang, Wenfeng, Hui Gong, Qianqian Sun, Yuting Fu, Xiaosi Wu, Hengwei Deng, Shaoping Weng, Jianguo He, and Chuanfu Dong. 2024. "Peripheral B Lymphocyte Serves as a Reservoir for the Persistently Covert Infection of Mandarin Fish Siniperca chuatsi Ranavirus" Viruses 16, no. 12: 1895. https://doi.org/10.3390/v16121895

APA Style

Zhang, W., Gong, H., Sun, Q., Fu, Y., Wu, X., Deng, H., Weng, S., He, J., & Dong, C. (2024). Peripheral B Lymphocyte Serves as a Reservoir for the Persistently Covert Infection of Mandarin Fish Siniperca chuatsi Ranavirus. Viruses, 16(12), 1895. https://doi.org/10.3390/v16121895

Note that from the first issue of 2016, this journal uses article numbers instead of page numbers. See further details here.

Article Metrics

Back to TopTop