Bactericera cockerelli Picorna-like Virus and Three New Viruses Found Circulating in Populations of Potato/Tomato Psyllids (Bactericera cockerelli)
Abstract
:1. Introduction
2. Materials and Methods
2.1. Psyllid and Plant Sampling and Sample Processing
2.2. RNA Extraction and HTS Analysis
2.3. Nucleic Acid Extraction, RT-PCR Testing, and Sanger Sequencing
2.4. Sequence and Phylogenetic Analysis
3. Results
3.1. Genome Analysis and Confirmation of the Presence of a New Tymo-like Virus in Psyllids
3.2. Genome Analysis and Confirmation of the Presence of New Solemo-like Viruses in Psyllids
3.3. Phylogenetic Analyses and Taxonomy of BcTLV, BcSLV-1, and BcSLV-2
3.4. BcPLV, BcTLV, and BcSLV-1 and -2 Can Be Found in Field-Collected Psyllids
4. Discussion
5. Conclusions
Supplementary Materials
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Acknowledgments
Conflicts of Interest
References
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Psyllid Sample ID a | Origin | Date Collected | Host Plant | Virus Sequences Found b | Largest Contig Size, nt | GenBank Accession Number |
---|---|---|---|---|---|---|
PSC | Wapato, WA | Feb-2021 | potato | PVS | 8546 | PP408647 |
BcPLV | 10,122 | PP408652 | ||||
PSW | Wapato, WA | Feb 2021 | potato/ tomato | BcTLV | 6843 | PP408654 |
PVS | 4339 | PP408649 | ||||
PVY | 3729 | PP408651 | ||||
BcPLV | 10,150 | PP408653 | ||||
PSNW | Wapato, WA | Feb 2021 | potato | PVS | 8537 | PP408650 |
BcSLV-2_1 | 3498 | PP408657 | ||||
BcSLV-2_2 | 3365 | PP408658 | ||||
KPF | Kimberly, ID | Aug 2020 | potato | PVS | 8407 | PP408648 |
BcSLV-1 | 5479 | PP408655 | ||||
BcSLV-2_1 | 3379 | PP408656 |
Date of Collection | BcPLV a | BcTLV a | BcSLV-1 a | BcSLV-2 a |
---|---|---|---|---|
7 July | 0/2 | 0/2 | 0/2 | 0/2 |
11 July | 1/3 | 0/3 | 2/3 | 1/3 |
14 July | 2/14 | 0/14 | 0/14 | 4/14 |
21 July | 8/10 | 0/10 | 0/10 | 6/10 |
14 August | 0/29 | 0/29 | 0/29 | 5/29 |
11 August | 0/23 | 1/23 | 0/23 | 12/23 |
15 August | 0/7 | 0/7 | 0/7 | 2/7 |
18 August | 9/44 | 1/44 | 5/44 | 29/44 |
25 August | 5/25 | 0/25 | 1/25 | 17/25 |
28 August | 21/28 | 0/28 | 1/28 | 19/28 |
1 September | 9/15 | 0/15 | 0/15 | 7/15 |
8 September | 1/6 | 2/6 | 0/6 | 3/6 |
14 September | 2/12 | 0/12 | 0/12 | 5/12 |
Total: | 58/218 | 4/218 | 9/218 | 110/218 |
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Dahan, J.; Orellana, G.E.; Wald, K.B.; Wenninger, E.J.; Cooper, W.R.; Karasev, A.V. Bactericera cockerelli Picorna-like Virus and Three New Viruses Found Circulating in Populations of Potato/Tomato Psyllids (Bactericera cockerelli). Viruses 2024, 16, 415. https://doi.org/10.3390/v16030415
Dahan J, Orellana GE, Wald KB, Wenninger EJ, Cooper WR, Karasev AV. Bactericera cockerelli Picorna-like Virus and Three New Viruses Found Circulating in Populations of Potato/Tomato Psyllids (Bactericera cockerelli). Viruses. 2024; 16(3):415. https://doi.org/10.3390/v16030415
Chicago/Turabian StyleDahan, Jennifer, Gardenia E. Orellana, Kaleigh B. Wald, Erik J. Wenninger, W. Rodney Cooper, and Alexander V. Karasev. 2024. "Bactericera cockerelli Picorna-like Virus and Three New Viruses Found Circulating in Populations of Potato/Tomato Psyllids (Bactericera cockerelli)" Viruses 16, no. 3: 415. https://doi.org/10.3390/v16030415