Assessment of Survival Kinetics for Emergent Highly Pathogenic Clade 2.3.4.4 H5Nx Avian Influenza Viruses
Abstract
:1. Introduction
2. Materials and Methods
2.1. Viruses and Safety Statement
2.2. Cell Lines
2.3. Preparation of Replicate Samples for Infectivity Assessment at Different Temperatures
2.4. Virus Quantification after Incubation at Different Temperatures
2.5. Statistical Analyses
3. Results and Discussion
4. Conclusions
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Acknowledgments
Conflicts of Interest
References
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Isolate | Subtype/Clade | Descriptor in Text | Collection Date 1 |
---|---|---|---|
A/duck/England/1279/2014 | H5N8 2.3.4.4a | H5N8-2014 | 16 November 2014 |
A/wigeon/Wales/52833/2016 | H5N8 2.3.4.4b | H5N8-2016 | 14 December 2016 |
A/swan/England/001986/2017 | H5N6 2.3.4.4b | H5N6-2017 | 31 December 2017 |
A/chicken/England/030786/2020 | H5N8 2.3.4.4b | H5N8-2020 | 1 November 2020 |
A/chicken/Wales/053969/2021 | H5N1 2.3.4.4b | H5N1-2021 | 31 October 2021 |
Temperature | 4 °C | 20 °C | 30 °C | ||||
---|---|---|---|---|---|---|---|
Strain | DT | Extinction 1 | DT | Extinction 1 | Strain | DT (days) | Extinction 1 |
H5N8-2020 | 24.2 | 116.1 | 8.0 | 38.5 | H5N8-2016 | 2.7 | 13.2 |
H5N8-2016 | 14.0 | 69.8 | 6.1 | 28.8 | H5N1-2021 | 2.3 | 8.6 |
H5N1-2021 | 9.5 | 43.2 | 4.5 | 17.2 | H5N8-2020 | 2.5 | 7.8 |
H5N6-2017 | 11.2 | 40.0 | 2.8 | 10.4 | |||
H5N8-2014 | 7.8 | 22.9 | 3.0 | 8.7 |
HPAIV | Result of Multiple Comparisons of DT Value/Extinction Time at 4 °C | ||||
---|---|---|---|---|---|
HPAIV Isolate | H5N8-2014 | H5N8-2016 | H5N6-2017 | H5N8-2020 | H5N1-2021 |
H5N8-2014 | - | <0.05/<0.001 | <0.001 | <0.001 | 0.33/<0.001 |
H5N8-2016 | - | - | 0.06/<0.001 | <0.001 | <0.001 |
H5N6-2017 | - | - | - | <0.001 | 0.22/0.58 |
H5N8-2020 | - | - | - | - | <0.001 |
H5N1-2021 | - | - | - | - | - |
HPAIV | Result of Multiple Comparisons of DT Value/Extinction Time at 20 °C | ||||
---|---|---|---|---|---|
HPAIV Isolate | H5N8-2014 | H5N8-2016 | H5N6-2017 | H5N8-2020 | H5N1-2021 |
H5N8-2014 | - | <0.01/<0.001 | 0.35/0.97 | <0.001 | 0.55/<0.05 |
H5N8-2016 | - | - | <0.001 | <0.01/<0.001 | <0.001 |
H5N6-2017 | - | - | - | <0.001 | <0.001/0.13 |
H5N8-2020 | - | - | - | - | <0.001 |
H5N1-2021 | - | - | - | - | - |
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Warren, C.J.; Brookes, S.M.; Arnold, M.E.; Irvine, R.M.; Hansen, R.D.E.; Brown, I.H.; Banyard, A.C.; Slomka, M.J. Assessment of Survival Kinetics for Emergent Highly Pathogenic Clade 2.3.4.4 H5Nx Avian Influenza Viruses. Viruses 2024, 16, 889. https://doi.org/10.3390/v16060889
Warren CJ, Brookes SM, Arnold ME, Irvine RM, Hansen RDE, Brown IH, Banyard AC, Slomka MJ. Assessment of Survival Kinetics for Emergent Highly Pathogenic Clade 2.3.4.4 H5Nx Avian Influenza Viruses. Viruses. 2024; 16(6):889. https://doi.org/10.3390/v16060889
Chicago/Turabian StyleWarren, Caroline J., Sharon M. Brookes, Mark E. Arnold, Richard M. Irvine, Rowena D. E. Hansen, Ian H. Brown, Ashley C. Banyard, and Marek J. Slomka. 2024. "Assessment of Survival Kinetics for Emergent Highly Pathogenic Clade 2.3.4.4 H5Nx Avian Influenza Viruses" Viruses 16, no. 6: 889. https://doi.org/10.3390/v16060889