Detection of Antibodies against Feline Morbillivirus by Recombinant Matrix Enzyme-Linked Immunosorbent Assay
Abstract
:1. Introduction
2. Materials and Methods
2.1. Sample Collection
2.2. Expression, Enrichment, and Identification of the Recombinant (r)FeMV-M Protein
2.3. Anti-FeMV Ab Screening by WB Analysis of the Serum Samples
2.4. Optimization and Detection of Anti-FeMV-M Abs by i-ELISA
2.5. Detection of FeMV RNA by Real-Time Reverse-Transcription Polymerase Chain Reaction (RT-qPCR)
2.6. Statistical Analysis
3. Results
3.1. Expression and Identification of His-rFeMV-M Protein and Serum Immunoblotting
3.2. Optimization of the His-rFeMV-M-Based i-ELISA and Its Application
3.3. Detection of FeMV RNA and Anti-FeMV-M Ab in Blood Samples
4. Discussion
Supplementary Materials
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Acknowledgments
Conflicts of Interest
References
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i-ELISA | WB | Total | |
---|---|---|---|
Positive | Negative | ||
Positive | 82 | 11 | 93 |
Negative | 9 | 34 | 43 |
Total | 91 | 45 | 136 |
Total (% coincidence) | 90.1% (82/91) | 75.6% (34/45) | 85.3% (116/136) |
RT-qPCR | FeMV Serology Results from Western Blotting | Total | |
---|---|---|---|
Positive | Negative | ||
Positive | 6 (RNA+/Ab+) | 5 (RNA+/Ab−) | 11 |
Negative | 85 (RNA−/Ab+) | 40 (RNA−/Ab−) | 125 |
Total | 91 | 45 | 136 |
Analyzed Method | Result | Shelter (n = 56) | Household (n = 80) | p Value |
---|---|---|---|---|
RT-qPCR | Positive | 11 * | 0 | <0.001 |
Negative | 45 | 80 | ||
Immunoblotting | Positive | 26 | 65 * | <0.001 |
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Share and Cite
Chaiyasak, S.; Piewbang, C.; Ratthanophart, J.; Techakriengkrai, N.; Rattanaporn, K.; Techangamsuwan, S. Detection of Antibodies against Feline Morbillivirus by Recombinant Matrix Enzyme-Linked Immunosorbent Assay. Viruses 2024, 16, 1339. https://doi.org/10.3390/v16081339
Chaiyasak S, Piewbang C, Ratthanophart J, Techakriengkrai N, Rattanaporn K, Techangamsuwan S. Detection of Antibodies against Feline Morbillivirus by Recombinant Matrix Enzyme-Linked Immunosorbent Assay. Viruses. 2024; 16(8):1339. https://doi.org/10.3390/v16081339
Chicago/Turabian StyleChaiyasak, Surangkanang, Chutchai Piewbang, Jadsada Ratthanophart, Navapon Techakriengkrai, Kittipong Rattanaporn, and Somporn Techangamsuwan. 2024. "Detection of Antibodies against Feline Morbillivirus by Recombinant Matrix Enzyme-Linked Immunosorbent Assay" Viruses 16, no. 8: 1339. https://doi.org/10.3390/v16081339