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Honeysuckle-Derived miR2911 Inhibits Replication of Porcine Reproductive and Respiratory Syndrome Virus by Targeting Viral Gene Regions
by
Xinyan Cao
Xinyan Cao 1,†,
Jiaxi Xue
Jiaxi Xue 1,†,
Adnan Ali
Adnan Ali 1,
Manyi Zhang
Manyi Zhang 2,
Jinliang Sheng
Jinliang Sheng 1,
Yanming Sun
Yanming Sun 1,*
and
Yanbing Zhang
Yanbing Zhang 1,2,*
1
College of Animal Science and Technology, Shihezi University, Shihezi 832003, China
2
Xinjiang Production & Construction Corps Key Laboratory of Animal biomedicine, Tumushuke 843900, China
*
Authors to whom correspondence should be addressed.
†
These authors contributed equally to this work.
Viruses 2024, 16(9), 1350; https://doi.org/10.3390/v16091350 (registering DOI)
Submission received: 18 July 2024
/
Revised: 21 August 2024
/
Accepted: 22 August 2024
/
Published: 23 August 2024
Abstract
The highly abundant and stable antiviral small RNA derived from honeysuckle, known as miR2911, has been shown to play a key role in inhibiting influenza virus infection and SARS-CoV-2 infection. However, whether miR2911 inhibits the replication of porcine reproductive and respiratory syndrome virus (PRRSV) remains unknown. Hence, this study investigated the mechanisms underlying the action of miR2911 during PRRSV infection. Six targets of miR2911 within the PRRSV orf1 (Nsp2: 2459 to 2477, 1871 to 1892, 954 to 977, and 1271 to 1292; Nsp1: 274 to 296 and 822 to 841) were successfully identified by using the miRanda v1.0b software. The miR2911 target sequence was analyzed by target sequence comparison, and only individual base mutations existed in different prevalent strains, and the miR2911 target region was highly conserved among different strains. Subsequently, through the dual luciferase reporter gene assay and miR2911 overexpression assay, it was demonstrated that miR2911 significantly inhibits the replication of PRRSV by targeting regions of PRRSV Nsp1 and Nsp2. These findings offer new insights for the development of novel anti-PRRSV drugs.
Share and Cite
MDPI and ACS Style
Cao, X.; Xue, J.; Ali, A.; Zhang, M.; Sheng, J.; Sun, Y.; Zhang, Y.
Honeysuckle-Derived miR2911 Inhibits Replication of Porcine Reproductive and Respiratory Syndrome Virus by Targeting Viral Gene Regions. Viruses 2024, 16, 1350.
https://doi.org/10.3390/v16091350
AMA Style
Cao X, Xue J, Ali A, Zhang M, Sheng J, Sun Y, Zhang Y.
Honeysuckle-Derived miR2911 Inhibits Replication of Porcine Reproductive and Respiratory Syndrome Virus by Targeting Viral Gene Regions. Viruses. 2024; 16(9):1350.
https://doi.org/10.3390/v16091350
Chicago/Turabian Style
Cao, Xinyan, Jiaxi Xue, Adnan Ali, Manyi Zhang, Jinliang Sheng, Yanming Sun, and Yanbing Zhang.
2024. "Honeysuckle-Derived miR2911 Inhibits Replication of Porcine Reproductive and Respiratory Syndrome Virus by Targeting Viral Gene Regions" Viruses 16, no. 9: 1350.
https://doi.org/10.3390/v16091350
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