Wastewater Surveillance of Mpox during the Summer Season of 2023 in Slovenia
Abstract
:1. Introduction
2. Materials and Methods
2.1. Mpox Monitoring Plan
2.2. Sampling and Transport of Samples
2.3. Implementation of the Microbiological Diagnostics
3. Results
4. Discussion
5. Conclusions
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Acknowledgements
Conflicts of Interest
References
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MPXV Inoculum Dilution | Sediment | Wastewater Sample | ||||||
---|---|---|---|---|---|---|---|---|
MPXV | EAV | MPXV | EAV | |||||
Undiluted | 1:10 | Undiluted | 1:10 | Undiluted | 1:10 | Undiluted | 1:10 | |
1:500 | 29.62 | 31.61 | 34.78 | 35.48 | 20.50 | 22.14 | 27.87 | 27.56 |
1:5000 | 33.41 | 36.66 | 34.04 | 35.15 | 24.21 | 26.31 | 27.95 | 27.78 |
1:50,000 | 32.72 | * | 35.25 | 35.49 | 26.90 | 29.36 | 27.30 | 28.24 |
1:500,000 | * | * | 35.74 | 36.28 | 30.12 | 31.81 | 28.16 | 28.08 |
1:5,000,000 | * | * | 35.38 | 36.48 | 33.89 | * | 27.98 | 28.10 |
Sampling Date | Wastewater Treatment Plant | Flow Rate [L/Day] | MPXV PCR Result |
---|---|---|---|
4 June 2023 | Ljubljana | 61,200,000 | Negative |
4 June 2023 | Koper | 12,360,000 | Negative |
11 June 2023 | Ljubljana | 89,426,000 | Negative |
12 June 2023 | Koper | 16,970,000 | Negative |
18 June 2023 | Ljubljana | 52,360,000 | Negative |
19 June 2023 | Koper | 13,600,000 | Negative |
25 June 2023 | Ljubljana | 43,388,000 | Negative |
26 June 2023 | Koper | 11,990,000 | Negative |
3 July 2023 | Ljubljana | 44,816,000 | Negative |
4 July 2023 | Koper | 14,382,000 | Negative |
10 July 2023 | Ljubljana | 45,783,000 | Negative |
11 July 2023 | Koper | 13,410,000 | Negative |
16 July 2023 | Ljubljana | 51,493,000 | Negative |
17 July 2023 | Koper | 12,990,000 | Negative |
23 July 2023 | Ljubljana | 44,415,000 | Negative |
24 July 2023 | Koper | 18,570,000 | Negative |
30 July 2023 | Ljubljana | 57,549,000 | Negative |
31 July 2023 | Koper | 15,410,000 | Negative |
6 August 2023 | Ljubljana | 138,822,000 | Negative |
7 August 2023 | Koper | 18,900,000 | Negative |
16 August 2023 | Ljubljana | 57,397,000 | Negative |
16 August 2023 | Koper | ** | Negative |
21 August 2023 | Ljubljana | 53,473,000 | Negative |
21 August 2023 | Koper | ** | Negative |
27 August 2023 | Ljubljana | 45,793,000 | Negative |
28 August 2023 | Koper | ** | Negative |
3 September 2023 | Ljubljana | 70,545,000 | Negative |
4 September 2023 | Koper | ** | Negative |
10 September 2023 | Ljubljana | 50,844,000 | Negative |
11 September 2023 | Koper | ** | Negative |
17 September 2023 | Ljubljana | 47,420,000 | Negative |
18 September 2023 | Koper | ** | Negative |
25 September 2023 | Ljubljana | 92,995,000 | Negative |
25 September 2023 | Koper | ** | Negative |
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Rožanec, J.; Kranjec, N.; Obid, I.; Steyer, A.; Cerar Kišek, T.; Koritnik, T.; Fafangel, M.; Galičič, A. Wastewater Surveillance of Mpox during the Summer Season of 2023 in Slovenia. Infect. Dis. Rep. 2024, 16, 836-845. https://doi.org/10.3390/idr16050065
Rožanec J, Kranjec N, Obid I, Steyer A, Cerar Kišek T, Koritnik T, Fafangel M, Galičič A. Wastewater Surveillance of Mpox during the Summer Season of 2023 in Slovenia. Infectious Disease Reports. 2024; 16(5):836-845. https://doi.org/10.3390/idr16050065
Chicago/Turabian StyleRožanec, Jan, Natalija Kranjec, Ivana Obid, Andrej Steyer, Tjaša Cerar Kišek, Tom Koritnik, Mario Fafangel, and An Galičič. 2024. "Wastewater Surveillance of Mpox during the Summer Season of 2023 in Slovenia" Infectious Disease Reports 16, no. 5: 836-845. https://doi.org/10.3390/idr16050065