First Molecular Detection and Characterization of Fowl Aviadenovirus Serotype 11 from Broiler Chickens in Chile
Round 1
Reviewer 1 Report
Comments and Suggestions for AuthorsThe authors made a good attempt to characterize the fowl adeno virus through this case report. Just need some minor editing as mentioned in the comment sections in the attached draft.
Comments for author File: Comments.pdf
Comments on the Quality of English LanguageEnglish looks OK, needs few minor editing.
Author Response
Thank you very much for taking the time to review this manuscript. Please find the detailed responses below and the corresponding revisions/corrections highlighted/in track changes in the re-submitted files.
Author Response File: Author Response.pdf
Reviewer 2 Report
Comments and Suggestions for AuthorsDear authors
Minor considerations need to be discussed.
The inclusion of a histopathologic study of the IBH cases are encouraged, if possible. Microscopic evaluation of livers with IBH-gross compatible lesions is necessary to determine the presence of basophilic intranuclear inclusion bodies, and link with gross lesions, PCR, sequencing and phylogenetic analysis.
The authors are also recommended to avoid the comparison and contrast with previously reports in which IBH/hydropericardium syndrome gross or microscopic findings were not included (e.g. Santander-Parra et al., 2023). Mixing the evaluation of FAdV isolates from both birds with and without IBH/hydropericardium syndrome lesions could drive to a wrong direction of the characterization of this viral agent.
Author Response
Thank you very much for taking the time to review this manuscript. Please find the detailed responses below and the corresponding revisions/corrections highlighted/in track changes in the re-submitted files.
Author Response File: Author Response.pdf
Reviewer 3 Report
Comments and Suggestions for AuthorsIt is scientifically significant to know the prevalance of FAdV around the planet. I would recommend the author to confirm the PCR results with one more (or two more) pair of primers specific for FAdV.
Line 144,145: The author hasnt explained the Negative control. Is it just the uninfected liver extract?
Author Response
Thank you very much for taking the time to review this manuscript. Please find the detailed responses below and the corresponding revisions/corrections highlighted/in track changes in the re-submitted files.
Author Response File: Author Response.pdf