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Microbiology Research
Volume 2
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10.4081/mr.2011.e7
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Article

A Novel Site of Insertion of IS6110 in the moaB3 Gene of a Clinical Isolate of Mycobacterium tuberculosis

by
Suma Sarojini
1,2,
GK Madhavilatha
1,
Smitha Soman
1,
R Ajay Kumar
1 and
Sathish Mundayoor
1,*
1
Mycobacterial Research Gtment of Molecuroup, Deparlar Microbiology, Rajiv Gandhi Centre for Biotechnology, Trivandrum, India
2
Department of Biotechnology, Christ University, Bangalore, India
*
Author to whom correspondence should be addressed.
Microbiol. Res. 2011, 2(1), e7; https://doi.org/10.4081/mr.2011.e7
Submission received: 6 October 2010 / Revised: 15 July 2010 / Accepted: 15 July 2010 / Published: 5 August 2011

Abstract

In Mycobacterium tuberculosis, genomic variation is generated mainly by insertions and deletions rather than by point mutations. RvD5 is one such deletion in M. tuberculosis H37Rv. Previous studies from our laboratory have shown the presence of moaA3 gene in the RvD5 region in a large number of clinical isolates, that is absent in M. tuberculosis H37Rv and H37Ra. The present study was aimed at investigating the RvD5 locus of the clinical isolates by a detailed PCR analysis. Here we report a new point of insertion of the mobile genetic element, IS6110 in the genome of one clinical isolate of M. tuberculosis. The insertion has disrupted the moaB3 gene, one of the ORFs in the RvD5 region, which is involved in the molybdopterin biosynthetic pathway. This insertion of IS6110 in the moaB3 of the clinical isolate is different when compared to the insertion in the moaB3 gene of M. tuberculosis H37Rv where 4kb RvD5 region has been lost by homologous recombination and only a truncated form of the gene is present. This finding is of relevance since IS6110 is a major element determining the genome plasticity of M. tuberculosis and its numerical and positional polymorphism has always been of special interest.
Keywords: IS6110; moaB3; Mycobacterium tuberculosis IS6110; moaB3; Mycobacterium tuberculosis

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MDPI and ACS Style

Sarojini, S.; Madhavilatha, G.; Soman, S.; Ajay Kumar, R.; Mundayoor, S. A Novel Site of Insertion of IS6110 in the moaB3 Gene of a Clinical Isolate of Mycobacterium tuberculosis. Microbiol. Res. 2011, 2, e7. https://doi.org/10.4081/mr.2011.e7

AMA Style

Sarojini S, Madhavilatha G, Soman S, Ajay Kumar R, Mundayoor S. A Novel Site of Insertion of IS6110 in the moaB3 Gene of a Clinical Isolate of Mycobacterium tuberculosis. Microbiology Research. 2011; 2(1):e7. https://doi.org/10.4081/mr.2011.e7

Chicago/Turabian Style

Sarojini, Suma, GK Madhavilatha, Smitha Soman, R Ajay Kumar, and Sathish Mundayoor. 2011. "A Novel Site of Insertion of IS6110 in the moaB3 Gene of a Clinical Isolate of Mycobacterium tuberculosis" Microbiology Research 2, no. 1: e7. https://doi.org/10.4081/mr.2011.e7

APA Style

Sarojini, S., Madhavilatha, G., Soman, S., Ajay Kumar, R., & Mundayoor, S. (2011). A Novel Site of Insertion of IS6110 in the moaB3 Gene of a Clinical Isolate of Mycobacterium tuberculosis. Microbiology Research, 2(1), e7. https://doi.org/10.4081/mr.2011.e7

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