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Microbiology Research
Volume 3
Issue 2
10.4081/mr.2012.e18
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Article

Cosynthesis and Different Expression Proteins Analysis of Antibiotic Synthesized-Blocked Mutants of Streptomyces Roseoflavus Men-myco-93-63

by
Li Aixia
1,2,3,
Meng Qing-Fang
1,2,3,
Chi Guo-Tong
1,2,3,
Shen Feng-Ying
1,2,3,
Ji Hong-Liu
1,2,3,
Li Ya-Ning
1,2,3,* and
Liu Da-Qun
1,2,3
1
College of Plant Protection, Agricultural University of Hebei, No.289, Lingyusi Street, Baoding 071001, China
2
National Engineering Research Center for Agriculture in Northern Mountainous Areas, Baoding 071001, China
3
Biological Control Center of Plant Disease and Plant Pests of Hebei Province, Baoding 071001, China
*
Author to whom correspondence should be addressed.
Microbiol. Res. 2012, 3(2), e18; https://doi.org/10.4081/mr.2012.e18
Submission received: 20 August 2011 / Revised: 1 April 2012 / Accepted: 11 April 2012 / Published: 24 July 2012

Abstract

Streptomyces roseoflavus Men-myco-93-63 was isolated from potato scab (S. scabies) decline soil as an antagonistic strain, which can effectively inhibit several phytopathogenic fungi and control important related plant diseases. In this study, cosynthesis and different expression protein analysis between seven antibiotic synthesized-blocked mutants of Men-myco-93-63 were carried out. Two-dimensional gel electrophoresis was used to compare proteins of cosynthesis and non-cosynthesis mutants. By matrix-assisted laser desorption/ionization time-of-flight mass spectrometry, two different protein spots were isolated and identified to be homologous proteins of ectoine hydroxylase and transcriptional regulator (DeoR family protein), which could be related to the biosynthesis of antibiotics of S. roseoflavus Men-myco-93-63.
Keywords: Streptomyces roseoflavus; blocked mutants; cosynthesis; two-dimensional gel electrophoresis; MALDI-TOF-MS Streptomyces roseoflavus; blocked mutants; cosynthesis; two-dimensional gel electrophoresis; MALDI-TOF-MS

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MDPI and ACS Style

Aixia, L.; Qing-Fang, M.; Guo-Tong, C.; Feng-Ying, S.; Hong-Liu, J.; Ya-Ning, L.; Da-Qun, L. Cosynthesis and Different Expression Proteins Analysis of Antibiotic Synthesized-Blocked Mutants of Streptomyces Roseoflavus Men-myco-93-63. Microbiol. Res. 2012, 3, e18. https://doi.org/10.4081/mr.2012.e18

AMA Style

Aixia L, Qing-Fang M, Guo-Tong C, Feng-Ying S, Hong-Liu J, Ya-Ning L, Da-Qun L. Cosynthesis and Different Expression Proteins Analysis of Antibiotic Synthesized-Blocked Mutants of Streptomyces Roseoflavus Men-myco-93-63. Microbiology Research. 2012; 3(2):e18. https://doi.org/10.4081/mr.2012.e18

Chicago/Turabian Style

Aixia, Li, Meng Qing-Fang, Chi Guo-Tong, Shen Feng-Ying, Ji Hong-Liu, Li Ya-Ning, and Liu Da-Qun. 2012. "Cosynthesis and Different Expression Proteins Analysis of Antibiotic Synthesized-Blocked Mutants of Streptomyces Roseoflavus Men-myco-93-63" Microbiology Research 3, no. 2: e18. https://doi.org/10.4081/mr.2012.e18

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