Next Article in Journal
Structural Character of Sorghum Endosperm Transfer Cells and Their Relationship with Embryo and Endosperm
Previous Article in Journal
A Simplified Method for Differential Staining of Aborted and Non-Aborted Pollen Grains
 
 
Search for Articles:
Title / Keyword
Author / Affiliation / Email
Journal
Article Type
 
 
Advanced Search
 
Section
Special Issue
Volume
Issue
Number
Page
 
Journals
IJPB
Volume 1
Issue 2
10.4081/pb.2010.e14
ijpb-logo
Submit to this Journal Review for this Journal Propose a Special Issue
► Article Menu

Article Menu

share Share announcement Help format_quote Cite question_answer Discuss in SciProfiles thumb_up
...
Endorse textsms
...
Comment
International Journal of Plant Biology is published by MDPI from Volume 13 Issue 1 (2022). Previous articles were published by another publisher in Open Access under a CC-BY (or CC-BY-NC-ND) licence, and they are hosted by MDPI on mdpi.com as a courtesy and upon agreement with PAGEPress.
first_page
settings
Order Article Reprints
Font Type:
Arial Georgia Verdana
Font Size:
Aa Aa Aa
Line Spacing:
Column Width:
Background:
Article

A Rapid Method for Isolation of Low-Molecular-Weight Rna from Arabidopsis Using Low Salt Concentration Buffer

by
Han Cheng
1,
Jing Gao
1,
Ze-Wei An
1 and
Hua-Sun Huang
1,2,*
1
Key Laboratory of Rubber Biology, Ministry of Agriculture, Rubber Research Institute, Chinese Academy of Tropical Agricultural Science, Danzhou 571737, China
2
Rubber Research Institute, Chinese Academy of Tropical Agricultural Science, Danzhou 571737, China
*
Author to whom correspondence should be addressed.
Int. J. Plant Biol. 2010, 1(2), e14; https://doi.org/10.4081/pb.2010.e14
Submission received: 23 January 2010 / Revised: 26 July 2010 / Accepted: 27 July 2010 / Published: 11 August 2010
Versions Notes

Abstract

We have developed a rapid extraction method using low salt concentration buffer for the isolation of low-molecular-weight RNA from Arabidopsis tissues. The method was quick and efficient, and the small scale extraction process took no more than 1 hour, while yield and RNA quality were comparable with those of previously reported. The LMW RNA isolated using this method was high quality, abundant in small RNA and free of high molecular weight RNA. This method can be used to extract low-molecular-weight RNA for the purpose of small RNA cloning and detection, and library construction.
Keywords: low-molecular-weight RNA; low salt concentration buffer; Arabidopsis low-molecular-weight RNA; low salt concentration buffer; Arabidopsis

Share and Cite

MDPI and ACS Style

Cheng, H.; Gao, J.; An, Z.-W.; Huang, H.-S. A Rapid Method for Isolation of Low-Molecular-Weight Rna from Arabidopsis Using Low Salt Concentration Buffer. Int. J. Plant Biol. 2010, 1, e14. https://doi.org/10.4081/pb.2010.e14

AMA Style

Cheng H, Gao J, An Z-W, Huang H-S. A Rapid Method for Isolation of Low-Molecular-Weight Rna from Arabidopsis Using Low Salt Concentration Buffer. International Journal of Plant Biology. 2010; 1(2):e14. https://doi.org/10.4081/pb.2010.e14

Chicago/Turabian Style

Cheng, Han, Jing Gao, Ze-Wei An, and Hua-Sun Huang. 2010. "A Rapid Method for Isolation of Low-Molecular-Weight Rna from Arabidopsis Using Low Salt Concentration Buffer" International Journal of Plant Biology 1, no. 2: e14. https://doi.org/10.4081/pb.2010.e14

Article Metrics

Back to TopTop