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Open AccessArticle
Agrobacterium tumefaciens-Mediated Genetic Transformation of Eclipta alba
1
Department of Bio-Sciences and Technology, M.M. Engineering College, Maharishi Markandeshwar (Deemed to be University), Mullana-Ambala 133207, India
2
Institute of Plant Sciences, Agricultural Research Organisation (ARO), The Volcani Center, Rishon LeZion 7505101, Israel
3
University Centre for Research and Development, University Institute of Pharmaceutical Sciences, Chandigarh University, Gharuan, Mohali 140413, India
*
Author to whom correspondence should be addressed.
Int. J. Plant Biol. 2024, 15(3), 641-651; https://doi.org/10.3390/ijpb15030047 (registering DOI)
Submission received: 11 June 2024
/
Revised: 3 July 2024
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Accepted: 5 July 2024
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Published: 10 July 2024
Abstract
Eclipta alba (Linn.) Hassk. (Asteraceae) is a high value medicinal plant which possesses diverse medicinal properties. It is an important herb for the treatment of various disorders, and is primarily used as a hepatoprotectant. Its major biochemical constituents include wedelolactone and dimethyl-wedelolactone (coumestans), which possess anti-hepatotoxic properties. Due to its numerous medicinal properties, it is in high demand by the pharmaceutical industry and therefore requires urgent biotechnological interventions for its improvement. Therefore, the present study was constructed with the aim of developing an efficient genetic transformation protocol for E. alba, which will help in the mass production of the active compounds found in E. alba. Agrobacterium tumefaciens strain LBA 4404, containing vector pBI121, was used to genetically transform the plant, and the effect of various factors such as infection type, light cycle effect, effect of pH, among others, on the genetic transformation efficiency was analyzed. Regenerated transformed shoots were confirmed using the standard Polymerase Chain Reaction PCR method. Kanamycin-resistant and beta- glucurosidaseGUS-positive shoots indicated the development of transgenic shoots in E. alba. Amplification of nptll and uidA genes confirmed the integration of t-DNA transgenic shoots. In conclusion, various factors affecting the transformation efficiency were analyzed, and a reliable A. tumefaciens-mediated genetic transformation protocol was developed.
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MDPI and ACS Style
Aggarwal, D.; Datta, V.; Tuli, H.S.; Kumar, P.; Ramniwas, S.
Agrobacterium tumefaciens-Mediated Genetic Transformation of Eclipta alba. Int. J. Plant Biol. 2024, 15, 641-651.
https://doi.org/10.3390/ijpb15030047
AMA Style
Aggarwal D, Datta V, Tuli HS, Kumar P, Ramniwas S.
Agrobacterium tumefaciens-Mediated Genetic Transformation of Eclipta alba. International Journal of Plant Biology. 2024; 15(3):641-651.
https://doi.org/10.3390/ijpb15030047
Chicago/Turabian Style
Aggarwal, Diwakar, Vasudha Datta, Hardeep Singh Tuli, Pawan Kumar, and Seema Ramniwas.
2024. "Agrobacterium tumefaciens-Mediated Genetic Transformation of Eclipta alba" International Journal of Plant Biology 15, no. 3: 641-651.
https://doi.org/10.3390/ijpb15030047
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