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Volume 3
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10.4081/pb.2012.e7
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International Journal of Plant Biology is published by MDPI from Volume 13 Issue 1 (2022). Previous articles were published by another publisher in Open Access under a CC-BY (or CC-BY-NC-ND) licence, and they are hosted by MDPI on mdpi.com as a courtesy and upon agreement with PAGEPress.
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Article

Optimization of Total RNA Isolation Method from the Aromatic Medicinal Plant Artemisia annua L.

by
Suganthi Appalasamy
,
Ning Shu Ping
,
Arvind Bhatt
,
Ahmad Sofiman Othman
,
Nad-Ali Babaeian Jelodar
and
Chan Lai Keng
*
Plant Tissue and Cell Culture Laboratory, School of Biological Sciences, University of Sains Malaysia, Penang 11800, Malaysia
*
Author to whom correspondence should be addressed.
Int. J. Plant Biol. 2012, 3(1), e7; https://doi.org/10.4081/pb.2012.e7
Submission received: 30 September 2011 / Revised: 11 July 2012 / Accepted: 23 July 2012 / Published: 10 December 2012

Abstract

This paper is the first report on the development of a protocol that allows rapid and simplified extraction of total RNA from Artemisia annua L., an aromatic medicinal plant. This innovative protocol ensures a consistently high quantity and good quality of total RNA without any contamination of polyphenols, polysaccharides and proteins. The total RNA obtained is also free of fungal RNA even when extracted from fungal infested plants. The extraction buffer used in the proposed modified protocol was made up of non-hazardous chemicals. High concentrations of polyphenols of A. annua L. could be successfully eliminated and the prepared total RNA could be used for downstream reactions.
Keywords: Artemisia annua L.; tissue cultured leaves; total RNA extraction; PCR Artemisia annua L.; tissue cultured leaves; total RNA extraction; PCR

Share and Cite

MDPI and ACS Style

Appalasamy, S.; Shu Ping, N.; Bhatt, A.; Othman, A.S.; Jelodar, N.-A.B.; Lai Keng, C. Optimization of Total RNA Isolation Method from the Aromatic Medicinal Plant Artemisia annua L. Int. J. Plant Biol. 2012, 3, e7. https://doi.org/10.4081/pb.2012.e7

AMA Style

Appalasamy S, Shu Ping N, Bhatt A, Othman AS, Jelodar N-AB, Lai Keng C. Optimization of Total RNA Isolation Method from the Aromatic Medicinal Plant Artemisia annua L. International Journal of Plant Biology. 2012; 3(1):e7. https://doi.org/10.4081/pb.2012.e7

Chicago/Turabian Style

Appalasamy, Suganthi, Ning Shu Ping, Arvind Bhatt, Ahmad Sofiman Othman, Nad-Ali Babaeian Jelodar, and Chan Lai Keng. 2012. "Optimization of Total RNA Isolation Method from the Aromatic Medicinal Plant Artemisia annua L." International Journal of Plant Biology 3, no. 1: e7. https://doi.org/10.4081/pb.2012.e7

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