Studies on Optimization of Sustainable Lactic Acid Production by Bacillus amyloliquefaciens from Sugarcane Molasses through Microbial Fermentation

Round 1

Reviewer 1 Report

Title	OK
Abstract	-          Line 15: lactic acid produce by microbes can also be racemic. Suggest adding ‘when specific microbial strains were used’ in the sentences
Introduction	-          Please include objective of your study -          Include reason why author want to isolate lactic acid producer from milk industry?
Materials and Methods	-          Figure 1: What are the definition of ‘before treatment’ and ‘after treatment’? Suggest including this information in the figure note. -          Figure 1: What are the differences in left picture and right picture? Please include explanation in Figure caption. -          Figure 2 was not mentioned anywhere in the manuscript. -          Line 90: why additional glucose was needed? The broth already have sugar inside? -          Line 91: what is the name of fermentation broth used here? Please include. -          Line 92: include temperature of room temperature. The might be different from room at different location.
Results and Discussion	-          Line 170: what is BCP-MRS method? In Figure 1 supplementary file shows TLC of Standard LA and Microbial LA, not BCP-MRS as mentioned by author. -          Line 184: include the feedback mechanisms in the manuscript. -          Suggest not to use the word supplementary file for Figure/ tables in the manuscript. -          Line 183: Why author use the term Plate instead of figure? -          Plate 1: Lactic acid production graph data is not clearly presented. -          Plate 2: What is control J2V2AA and J2V4AA means? -          Line 204: What do you mean by non-fastidious Bacillus amyloliquefaciens? -          Line 217: include the figure caption for the figure in manuscript. -          Line 230 – 231: inhibition due to high sugar concentration. -          Line 222: suggest to change the subtopic title to the effect of sugarcane molasses concentration on… -          Table 1: please include result from this study in this table.
Overall	-          Strongly suggest for professional English proofread -          Please correct the typing error for the use of capital letter in the manuscript. -          No statistical analysis was conducted in the manuscript. Suggest to include statistical analysis to strengthen authors data. -

Author Response

 "Please see the attachment."

Author Response File: Author Response.pdf

Reviewer 2 Report

1.        Giving the section “Abbreviation” in the begining of manuscript

2.        Page 2, line 83: giving the meaning of BCP-MRS.

3.        Page 3, line 97: specification of pilot scale? I think that the statement of this paragraph is not very clear.

4.        Page 4, line 144: fresh 0.3% Fecl3 solutions=> fresh 0.3% FeCl₃ solutions

5.        Page 6, line 190: Plate 1: Screening… => Figure 1: Screening…

6.        Page 7, line 202: Plate 2: Pilot Scale…. => Figure 2: Pilot Scale….

7.        Page 7, line 210: Plate 3: Phylogenetic…=> Figure 3: Phylogenetic…

8.        In the manuscript, many Figures are lack of Title. Please check?

9.        Page 9, line 222: Effect of different concentration Sugarcane Molasses on LA Production should be discussed clearly.

 

Author Response

 "Please see the attachment."

Author Response File: Author Response.pdf

Round 2

Reviewer 1 Report

Abstract

-          Line 19: Remove the in “… This study the produced sustainable lactic acid…”

Introduction

-          Line 59 – 62: I would suggest to remove the result statement from introduction section as “… In this study, high lactic acid production has been achieved through fermentation using sugarcane molasses as a carbon source by Novel Non-fastidious Bacillus amyloliquefaciens strain J2V2AA isolated from milk industry effluent [18]..”

Material and Methods

-          Figure 1: Suggest the author to not mixed up the location for isolation lactic acid producing bacteria and location for sugarcane molasses. Location for sugarcane molasses should be included under Subtopic 2.3. Suggest to rename the Figure 1 caption to: Location for milk effluent sample collection.

-          Line 92 – 93: please correct grammatical and typing error in the sentences. What do the author means by minimal SM concentration? I would suggest to include the minimum value in the text.

Result and discussions:

-          No statistical analysis was included in the figure to determine the significant different or not. Normally, the significant difference of sample

-          Line 177 – 178: please include explanation on feedback mechanisms.

-          Line 226 – 227: Please rewrite the Figure 7 captions as a title. Do not include result in the caption. Label A, B and C in the figure box. No need to include right, left, down.

-           

Overall

-          Many grammatical and typing errors such as capital letter for general words, suggest the author to re-check the whole manuscript

-          Example: Line 92 – 93: “… as a Carbon Source for the LA Production Pilot-scale experiment was carried out with minimal SM concentration…”

 

Author Response

"Please see the attachment."

Author Response File: Author Response.pdf

Reviewer 2 Report

1.        Page 2, line 46: ,Various researchers….. => Various researchers…..

2.        Page 2, line 48:….. acid [11],[12]. =>…….. acid [11,12].

3.        Page 3, line 94: size of pilot scale? Inoculum?

4.        Page 4, line 101: Therefore, Optimization….=> Therefore, optimization….

5.        Page 4, line 137: Vis Spectrophotometer)… => Vis Spectrophotometer…

6.        Page 7, line 202: Title of Figure 5-9 should be located at the bottom of Figures.

 

Author Response

"Please see the attachment."

Author Response File: Author Response.pdf