Effects of the Application of Nutrients on Soil Bacterial Community Composition and Diversity in a Larix olgensis Plantation, Northeast China
Abstract
:1. Introduction
2. Materials and Methods
2.1. Site Description
2.2. Experimental Design and Soil Sample Collection
2.3. Soil Chemical Properties and Biological Analysis
2.3.1. Chemical Properties Analysis
2.3.2. DNA Extraction and PCR Amplification
2.4. Bioinformatics
2.5. Statistical Analyses
3. Results
3.1. Characteristics of Soil Bacterial Diversity under Different Nutrient Application Measures
3.2. Effects of the Application of Nutrients on Soil Bacterial Community Composition
3.3. The Relationship between the Soil Bacterial Community Structure and Soil Chemical Properties
4. Discussion
4.1. Response of Soil Chemical Properties to the Different Nutrient Application Regimes
4.2. Soil Bacterial Diversity under Nutrient Application Regimes
4.3. Changes in the Main Soil Bacterial Community under Different Nutrient Application Measures
5. Conclusions
Supplementary Materials
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Acknowledgments
Conflicts of Interest
References
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Treat. | (g/kg) Total N | (g/kg) Total P | (g/kg) Total K | (g/kg) Organic C | (mg/kg) Available P | (mg/kg) Available K | pH |
---|---|---|---|---|---|---|---|
CK | 2.48 ± 0.28 a | 0.95 ± 0.21 a | 5.95 ± 1.85 a | 60.63 ± 22.53 a | 14.93 ± 6.72 a | 109.77 ± 33.09 b | 4.81 ± 0.25 a |
N | 2.98 ± 1.01 a | 0.93 ± 0.44 a | 3.04 ± 1.48 b | 58.27 ± 8.90 a | 12.47 ± 4.55 a | 149.94 ± 29.04 b | 4.54 ± 0.30 ab |
NP | 2.33 ± 0.76 a | 0.97 ± 0.23 a | 3.65 ± 1.00 ab | 62.44 ± 15.66 a | 18.74 ± 8.82 a | 199.15 ± 27.26 ab | 4.43 ± 0.04 b |
NPK | 3.62 ± 0.89 a | 1.09 ± 0.37 a | 4.61 ± 1.13 ab | 59.64 ± 23.58 a | 15.49 ± 4.12 a | 286.15 ± 68.08 a | 4.55 ± 0.02 ab |
Treat. | Chao1 Index | Shannon Index | Richness Index | Coverage |
---|---|---|---|---|
CK | 1717.01 ± 35.851 b | 5.83 ± 0.090 a | 1519.00 ± 42.46 a | 0.99 ± 0.00 a |
N | 1790.50 ± 32.010 a | 5.80 ± 0.107 a | 1549.33 ± 36.23 a | 0.99 ± 0.00 a |
NP | 1781.14 ± 23.490 a | 5.75 ± 0.108 a | 1544.67 ± 41.04 a | 0.99 ± 0.00 a |
NPK | 1772.19 ± 39.659 ab | 5.81 ± 0.107 a | 1549.67 ± 13.58 a | 0.99 ± 0.00 a |
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Cui, J.; Sun, Z.; Wang, Z.; Gong, L. Effects of the Application of Nutrients on Soil Bacterial Community Composition and Diversity in a Larix olgensis Plantation, Northeast China. Sustainability 2022, 14, 16759. https://doi.org/10.3390/su142416759
Cui J, Sun Z, Wang Z, Gong L. Effects of the Application of Nutrients on Soil Bacterial Community Composition and Diversity in a Larix olgensis Plantation, Northeast China. Sustainability. 2022; 14(24):16759. https://doi.org/10.3390/su142416759
Chicago/Turabian StyleCui, Jinyao, Zhihu Sun, Zixuan Wang, and Lifang Gong. 2022. "Effects of the Application of Nutrients on Soil Bacterial Community Composition and Diversity in a Larix olgensis Plantation, Northeast China" Sustainability 14, no. 24: 16759. https://doi.org/10.3390/su142416759