Green Synthesis of Gold and Silver Nanoparticles Using Invasive Alien Plant Parthenium hysterophorus and Their Antimicrobial and Antioxidant Activities
, Siraye Esubalew Debebe 1,2, Archana Bachheti 3, Yashwant S. Rawat 4 and Rakesh Kumar Bachheti 1,2,*
Round 1
Reviewer 1 Report
Rakesh Kumar Bachheti et al. described the synthesis of silver and gold nanoparticles by using the plant extract of Parthenium hysterophorus and exploited its application as antimicrobial, antifungal, and antioxidant properties. In this work, the authors claimed that the use of plant extract not only reduced the cationic silver/gold metal but also acted as a caped reagent to have their stabilization. The authors used UV-Vis, IR spectroscopy, and DLS to characterize Nanoparticles. The TEM imaging and XRD were also used to corroborate the particle size of Nanoparticles. Please consider the following comments.
1. On page 3, line 96, the authors claimed that simultaneous studies for synthesizing silver and gold NP had not been studied with antibacterial, antifungal, and antioxidant properties. There are several reports on synthesizing silver nanoparticles using the plant extract of Parthenium hysterophorus.
o J Clust Sci 2021, 32, 167–177
o Digest Journal of Nanomaterials and Biostructures 2009, 4, 45 – 50
o Int. J. Curr. Microbiol. App. Sci 2013, 2, 220-227
o Molecules 2020, 25, 3324; doi:10.3390/molecules25153324
There is a need to mention that silver nanoparticle synthesis and its biological evaluation were explored. And authors report the synthesis of Gold nanoparticles, which nobody has reported so far.
2. Though it is well explored that the plant extract of Parthenium hysterophorus is acting as a reducing agent, it is worth exploring its mechanism or biological pathways as a reducing agent. Cyclic voltammetry is recommended for studying this kind of reduction mechanism.
3. Using the bar charts for a better understanding of the biological activity is recommended.
4. Are any ATCC numbers available for the bacterial/fungus used in the biological activity?
5. The quality of TEM Images needs to be improved (High-resolution images are recommended)
6. It is recommended to include the control zone inhibition in Table 2.
7. Using the multiplication sign-in table 3 is recommended, so avoid using *.
Author Response
Response to the comments of the reviewers
Author Response File: 
Author Response.pdf
Reviewer 2 Report
1. I would recommend that the authors emphasize the novelty of their work. The cited work, "Green Synthesis of Silver Nanoparticles Using Parthenium Hysterophorus: Optimization, Characterization and In Vitro Therapeutic Evaluation" (Molecules, 2020, 25(15), 3324), discusses research very similar to this study, including the synthesis method, particle characterization, and antimicrobial property analysis. Therefore, it is crucial to demonstrate the originality and significance of the work in this manuscript.
2. I suggest that the authors clarify in the Abstract that the particle size measured by DLS is the hydrodynamic size to avoid confusion with the particle size measured by TEM.
3. There is a typo in Figure 5 (XRD). The legends for both figures read "AgNPs."
Author Response
Response to the comments of the reviewers
Author Response File: Author Response.pdf
Reviewer 3 Report
The article is and devoted to green synthesis of Au and Ag nanoparticles on base Parthenium hysterophorus, investigatation their antimicrobial and antioxidant activity is interesting . The work will be useful specialists in this field of science. There are some minor comments on the work, which are highlighted in yellow in the attached file. I recommend the article be published after minor corrections.
Comments for author File: Comments.pdf
Author Response
Response to the comments of the reviewers
Author Response File: Author Response.pdf
Round 2
Reviewer 2 Report
Accept in present form
