Chitin Oligosaccharide N,N′-Diacetylchitobiose (GlcNAc2) as Antimicrobial Coating against Listeria monocytogenes on Ready-to-Eat Shrimp
Round 1
Reviewer 1 Report
Please see the attached file
Comments for author File: 
Comments.pdf
Minor corrections are needed. It is recommended to review entire manuscript for English corrections.
Author Response
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Author Response File: Author Response.pdf
Reviewer 2 Report
The article 'Chitin oligosaccharides, N,N’-diacetylchitobiose (GlcNAc2) as antimicrobial coating against Listeria monocytogenes on ready-to-eat shrimp' is highly beneficial piece of research. It makes a significant contribution to our understanding of food safety and shelf-life extension strategies in the seafood industry.
The authors provide a thorough investigation into the use of chitin oligosaccharides, specifically N,N’-diacetylchitobiose (GlcNAc2), as a protective antimicrobial coating on ready-to-eat shrimp. Their detailed analysis and experimental methodology are commendable, demonstrating a robust approach to the investigation of such a critical topic.
Their results, showing the significant potential of GlcNAc2 in inhibiting the growth of Listeria monocytogenes, provide valuable insights that could be pivotal in the design of new, sustainable methods for seafood preservation. Furthermore, the article is well-structured and well-written, making the complex scientific data accessible to a broad audience.
The authors' focus on a biodegradable and non-toxic compound like GlcNAc2 also aligns with the increasing demand for sustainable and safe food preservation technologies.
I have few suggestions to improve the manuscript:
1. Section Materials and Methods: add paragraph Chemicals, indicating all reagents used, their purity and CAS.
2. Section Materials and Methods: add paragraph Strains, below chemicals adding information about Listeria etc.
3. Section 2.4.2. Colour: how many measurements of each sample have been taken?
4. Section 2.4.4. Lipid peroxidation: how many concentration points had a calibration curve of mda? What was correlation coefficient (r) of the curve? Against which blank spectrophotometric measurement was done ?
5. Figure 2: write unit of TBARS in brackets (mg malonaldehyde/kg shrimp)
6. Figure 3. write unit of Moisture content changes in brackets
Author Response
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Author Response File: Author Response.pdf
Reviewer 3 Report
The manuscript “Chitin oligosaccharides, N,N’-diacetylchitobiose (GlcNAc2) as antimicrobial coating against Listeria monocytogenes on ready-to-eat shrimp N,N’-diacetylchitobios” contains sufficient data for the development of the food industry and the scientific results are in full agreement with the aim and scope of the journal. The authors have studied N,N’-diacetylchitobiose (GlcNAc2) as an antimicrobial coating against L. monocytogenes for on ready-to-eat shrimp by monitoring of the texture properties, colour, moisture content and bacterial counts for some days. The study shows that the GlcNAc2 used as an antimicrobial coating was able to inhibit the growth of L. monocytogenes, while maintaining the quality of the ready-to-eat shrimp during refrigerated storage. The manuscript is well and carefully written, data are solid and conclusions are justified by the results. The discussions on the primary results and their significance are scientifically sound. I recommend to accept Ms in the current form.
Author Response
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Author Response File: Author Response.pdf
Round 2
Reviewer 1 Report
Thanks for answering all minor comments. Yet i recommend authors to answer few critical comments highlighted in yellow in the attached file. Addition of those answers will improve this manuscript significantly and hence can be publishable in this journal.
Comments for author File: Comments.pdf
Author Response
Please see the attachment.
Author Response File: Author Response.pdf
