Molecular Identification of Endophytic Bacteria from Silybum marianum and Their Effect on Brassica napus Growth under Heavy Metal Stress
, Sajjad Ullah Khan 2,†, Ihsan Ullah 1, Hassan A. Hemeg 3, Rahma Ashamrani 1, Nadiah Al-sulami 1, Ezzudin Ghazi Alniami 4, Mohammed Hashem Alqethami 1 and Abrar Ullah 1
Round 1
Reviewer 1 Report
Overall, the manuscript well written, with many area can be improved. I suggest several aspect on method, discussion and introduction part. Hope the suggestion can help.
Comments for author File: 
Comments.pdf
Author Response
Respected Reviewer,
Hope everything will be fine on your side.
Thank you for the nice comments. Your comments make our paper in a good condition.
We try our best to Review the Manuscript according to your kind instructions.
Thank you Again
Be happy and have a nice day
With regards
Yasir Anwar
Author Response File: Author Response.pdf
Reviewer 2 Report
Dear Drs, authors
Happy day and happy years.
The authors try to link between the presence of the heavy metals represented in the Cr with the ability to enhance the growth and as they say the health of the plants.
They trying to link between mainly three variables, the plant, heavy metals (Cr) as well as the entophytic bacteria.
The subject and the idea is cleaver and interesting.
But some critical points need to be highlighted.
- The antimicrobial effect of the Cr (used quantity) against both of the entophytic bacteria and the standard used microbes.
- Images from the antagonistic activates should be included.
- In future study the authors should determine the LC50 (ID50) of the Cr on the bacterial cells (not needed in this study).
- The text in some parts need references.
- The authors are advised to avoid the use of over estimated words. Or use the words that reflect an opinion. Meanwhile, they can use words such as, "apparently, proposed and the like.
I found the paper in general nice and accepted, but one week work in its style might improve it significantly.
With my pleasure
Amro Amara
Comments for author File: Comments.pdf
Author Response
Respected Reviewer,
Hope everything will be fine on your side.
Thank you for the nice comments. Your comments make our paper in a good condition.
We try our best to Review the Manuscript according to your kind instructions.
Thank you Again
Be happy and have a nice day
With regards
Yasir Anwar
Author Response File: Author Response.pdf
Reviewer 3 Report
Explanations are required in the material and methods section to clarify the methods used. The material and methods should state which microorganisms were used for antimicrobial testing, with detailed information about the strains used. Did the culture from the discs develop around the disc inhibiting the development of other bacteria present in the agar plate, or the development of the agar-present bacteria was absent around the disc? Please define in detail how the inhibition zones were measured. Additionally, did the authors perform positive control using the same bacteria in the agar plate against the same strain in the discs? A photo would be useful.
How was the bacterial culture added to the pots? 2 ml is a low volume, was this inoculate dissolved in large volume and applied, and were roots soaked in inoculate? How did the authors ensure the inoculation of bacterial culture to the test plants? Was any confirmation performed to identify whether the inoculate was successfully becoming an endophyte of the test plant, or are the effects just a result of the presence of the bacteria in the growth medium?
Author Response
Respected Reviewer,
Hope everything will be fine on your side.
Thank you for the nice comments. Your comments make our paper in a good condition.
We try our best to Review the Manuscript according to your kind instructions.
Thank you Again
Be happy and have a nice day
With regards
Yasir Anwar
Author Response File: Author Response.pdf
Round 2
Reviewer 3 Report
Dear Authors,
in your statement, you confirmed that you addressed my comments. Unfortunately in the version that you uploaded non of my significant comments were actually addressed. I advise you to incorporate your response in the manuscript before resubmission.
Author Response
Respected Reviewer,
we try our best to revise the paper according to your kind comments.
Be happy and have a nice day
with regards
Yasir Anwar
Author Response File: Author Response.pdf
Round 3
Reviewer 3 Report
The authors addressed the comments effectively.
Best regards
