Microbial Protein Production Using Lignocellulosic Biomass (Switchgrass) and Klebsiella oxytoca M5A1—A Nitrogen Fixer
Abstract
:1. Introduction
2. Methodology
2.1. Media
2.2. Culturing of Klebsiella oxytoca M5A1
2.3. Biomass Pretreatment
2.4. Enzymatic Hydrolysis of Biomass
2.5. Analytical Methods: Glucose Utilization and Organic Acids Production
2.6. Fermentation Conditions and Growth Profiling
2.7. Protein Quantification
2.8. Acetylene Reduction Assay
2.9. Statistical Analysis
3. Result and Discussion
3.1. Growth Profile K. oxytoca M5A1 in Switchgrass Hydrolysate
3.2. Glucose Utilization of K. oxytoca M5A during Fermentation
3.3. MP Content of K. oxytoca M5A1
3.4. Nitrogen-Fixing Capability of K. oxytoca M5A1
3.5. Organic Acids Profile during Fermentation to Protein
4. Conclusions
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Conflicts of Interest
References
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Ayodele, T.; Alarape, K.; Bello, I.A.; Tijani, A.; Musiliu, L.; Hammed, A. Microbial Protein Production Using Lignocellulosic Biomass (Switchgrass) and Klebsiella oxytoca M5A1—A Nitrogen Fixer. Sustainability 2024, 16, 5486. https://doi.org/10.3390/su16135486
Ayodele T, Alarape K, Bello IA, Tijani A, Musiliu L, Hammed A. Microbial Protein Production Using Lignocellulosic Biomass (Switchgrass) and Klebsiella oxytoca M5A1—A Nitrogen Fixer. Sustainability. 2024; 16(13):5486. https://doi.org/10.3390/su16135486
Chicago/Turabian StyleAyodele, Tawakalt, Kudirat Alarape, Ibrahim Adebayo Bello, Abodunrin Tijani, Liadi Musiliu, and Ademola Hammed. 2024. "Microbial Protein Production Using Lignocellulosic Biomass (Switchgrass) and Klebsiella oxytoca M5A1—A Nitrogen Fixer" Sustainability 16, no. 13: 5486. https://doi.org/10.3390/su16135486