Valorization of Hibiscus Flower (Hibiscus sabdariffa L.) Anthocyanins to Produce Sustainable Spray-Dried Ingredients

Round 1

Reviewer 1 Report

Comments and Suggestions for Authors

 

This manuscript presents an interesting study on applying hibiscus flowers (Hibiscus sabdariffa L.) anthocyanins to produce sustainable spray-dried ingredients based on optimizing the extraction and spray-drying processes, maintaining the antioxidant properties of the material. The manuscript is generally well-written, with an objective and well-contextualized introduction to the subject. The methodology is detailed and reproducible. The results were well presented; however, a deeper discussion is necessary to highlight the main scientific contributions achieved in this study further. Including more comparisons with previous studies is an option for a better discussion of the results. Therefore, the manuscript requires a minor review before being accepted for publication.

Comments to authors

Materials and methods

-Line 76: Was the choice of hammer mill and cyclone sizes based on previous studies? If yes, add a reference. Otherwise, justify the sizes used;

-Line 90: Display the centrifugation speed as 4000 x g (g in italics);

-Lines 81 and 108: I suggest that authors present the experimental planning information separately from the results so that information such as factors levels and real values ​​used are made clear, which will contribute to a better understanding by the reader.

Results and discussion

I suggest correlating the results with previous literature investigations to make the discussion more interesting and complete. Give greater emphasis to the advances achieved with this investigation so that the paper does not become purely statistical.

 

 

Author Response

Please find the responses in the attached document

Author Response File: Author Response.pdf

Reviewer 2 Report

Comments and Suggestions for Authors

The manuscript deals with an interesting topic of extraction and spray drying of anthocyanins recovered from hibiscus flowers (Hibiscus sabdariffa L.) to produce extracts with preserved phenolic antioxidants.

 

Below, I offer some minor remarks which should be addressed before manuscript publication:

I would think it reasonable to carry out the DPPH, ABTS antioxidant analysis of the extract.

 

Lines 260 to 268 is material and methods.

 

Lines 269-278:  Discuss the results with other works.

Author Response

Please find the responses in the attached document.

Author Response File: Author Response.pdf

Reviewer 3 Report

Comments and Suggestions for Authors

Dear authors,

In my opinion, the topic of your research is interesting and useful for further research dealing with the investigation of herbs and different natural products as sustainable anthocyanins supply to produce spray dried ingredients with antioxidant capacity. Your paper is exceptionally well-written and easy to follow, demonstrating your expertise through the references you have cited.

I have a few technical suggestions that I believe could enhance the quality of your paper:

1. To facilitate comparison and tracking, I recommend including an explanation of the abbreviations for the following items:

Table 2- abbreviation for HFF

Figure 1- abbreviations for ANC and C3G/L

Figure 2- abbreviation for ANC and HFF

2. In Table S1, I suggest incorporating three levels of significance:

* for p<0.5, ** for p<0.01 and *** for p<0.001.

 

Author Response

Please find the responses in the attached document.

Author Response File: Author Response.pdf