Screening and Physiological Responses of Maize Inbred Lines to Drought Stress in South China
Abstract
:1. Introduction
2. Materials and Methods
2.1. Plant Material and Experimental Treatments
2.2. The First Experiment
2.3. The Second Experiment
2.4. Sampling and Measurements
2.4.1. Chlorophyll Contents
2.4.2. Morphological Parameters
2.4.3. Relative Water Content
2.4.4. Photosynthetic Pigment
2.4.5. Antioxidant Activity
2.4.6. Malondialdehyde Content
2.4.7. Proline Content
2.5. Tolerance Analysis
2.6. Statistical Analysis
3. Results
3.1. Response of Maize Seedling Traits to Drought Stress
3.2. Comprehensive Evaluation of D Value
3.3. Survival Rate Analysis
3.4. Principal Component Analysis
3.5. Correlation Analysis
3.6. Cluster Analysis
3.7. Phenotypic Differences in Drought Response between Two Maize Inbred Lines
3.8. Differences in Physiological Indices between Two Maize Inbred Lines under Drought Stress
4. Discussion
5. Conclusions
Supplementary Materials
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Acknowledgments
Conflicts of Interest
Abbreviations
References
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Indicator | MAX | MIN | Mean ± SD | CV (%) | TC | CV of TC (%) | ||||
---|---|---|---|---|---|---|---|---|---|---|
WW | DS | WW | DS | WW | DS | WW | DS | Mean | ||
SPAD | 50.43 | 46.60 | 35.90 | 23.80 | 41.84 ± 2.39 a | 37.70 ± 3.11 b | 5.71 | 8.24 | 0.90 | 6.16 |
PH (cm) | 154.80 | 117.27 | 81.10 | 64.23 | 122.18 ± 11.12 a | 94.39 ± 8.18 b | 9.03 | 8.67 | 0.77 | 8.47 |
PFW (g) | 88.69 | 43.93 | 25.10 | 7.83 | 59.95 ± 11.49 a | 23.97 ± 6.30 b | 19.14 | 26.30 | 0.41 | 29.71 |
PDW (g) | 8.21 | 5.39 | 1.50 | 1.14 | 5.39 ± 1.04 a | 3.68 ± 0.69 b | 19.26 | 18.63 | 0.69 | 18.69 |
RFW (g) | 25.70 | 7.36 | 2.32 | 1.05 | 5.59 ± 1.86 a | 3.67 ± 0.97 b | 33.37 | 26.43 | 0.69 | 23.43 |
RDW (g) | 1.22 | 0.89 | 0.24 | 0.08 | 0.59 ± 0.18 a | 0.43 ± 0.16 b | 30.28 | 36.57 | 0.74 | 30.16 |
RL (cm) | 59.47 | 79.77 | 20.97 | 31.57 | 38.18 ± 15.72 b | 48.72 ± 6.61 a | 15.72 | 13.57 | 1.29 | 13.65 |
RCR | 0.25 | 0.61 | 0.04 | 0.06 | 0.09 ± 0.03 b | 0.16 ± 0.07 a | 32.18 | 40.32 | 1.79 | 35.10 |
Indicator | Principal Component | Weight of Principal Component | Total | ||||
---|---|---|---|---|---|---|---|
F1 | F2 | F3 | F1 | F2 | F3 | F | |
SPAD | 0.04 | 0.06 | −0.81 | 0.01 | 0.02 | 0.38 | 0.08 |
PH (cm) | −0.07 | 0.73 | 0.10 | 0.02 | 0.20 | 0.05 | 0.10 |
PFW (g) | −0.01 | 0.89 | 0.04 | 0.00 | 0.25 | 0.02 | 0.10 |
PDW (g) | 0.31 | 0.80 | 0.00 | 0.09 | 0.22 | 0.00 | 0.13 |
RFW (g) | 0.87 | 0.17 | 0.16 | 0.25 | 0.05 | 0.08 | 0.14 |
RDW (g) | 0.88 | −0.09 | 0.08 | 0.25 | 0.02 | 0.04 | 0.12 |
RL (cm) | 0.51 | 0.17 | −0.22 | 0.15 | 0.05 | 0.10 | 0.10 |
RCR | 0.70 | −0.57 | 0.12 | 0.20 | 0.16 | 0.06 | 0.16 |
SR | 0.09 | 0.14 | 0.58 | 0.03 | 0.04 | 0.28 | 0.08 |
Eigenvalue | 2.45 | 2.36 | 1.07 | ||||
CR (%) | 27.20 | 26.24 | 11.88 | ||||
CCR (%) | 27.20 | 53.44 | 65.32 |
Inbred Line | Days | Plant Height (cm) | Stem Diameter (mm) | Leaf Area (cm2) |
---|---|---|---|---|
CML323 | 0 | 39.35 ± 1.02 b | 3.07 ± 0.08 b | 24.96 ± 6.21 a |
1 | 42.85 ± 3.38 b | 3.31 ± 0.10 b | 24.62 ± 5.56 a | |
3 | 50.6 ± 4.73 a | 3.74 ± 0.40 a | 23.27 ± 2.27 a | |
5 | 50.1 ± 1.95 a | 3.63 ± 0.13 a | 21.29 ± 0.84 a | |
CB2-49-1 | 0 | 36.78 ± 2.73 c | 3.05 ± 0.22 c | 31.93 ± 5.41 a |
1 | 38.98 ± 2.66 ab | 3.19 ± 0.18 bc | 29.01 ± 1.92 ab | |
3 | 42.15 ± 2.15 a | 3.47 ± 0.22 a | 29.09 ± 5.26 ab | |
5 | 41.83 ± 3.22 a | 3.41 ± 0.13 ab | 26.00 ± 3.12 b |
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Zhang, Z.; Xie, X.; Naseer, M.A.; Zhou, H.; Cheng, W.; Xie, H.; Qin, L.; Yang, X.; Jiang, Y.; Zhou, X. Screening and Physiological Responses of Maize Inbred Lines to Drought Stress in South China. Sustainability 2024, 16, 7366. https://doi.org/10.3390/su16177366
Zhang Z, Xie X, Naseer MA, Zhou H, Cheng W, Xie H, Qin L, Yang X, Jiang Y, Zhou X. Screening and Physiological Responses of Maize Inbred Lines to Drought Stress in South China. Sustainability. 2024; 16(17):7366. https://doi.org/10.3390/su16177366
Chicago/Turabian StyleZhang, Zhiqin, Xiaodong Xie, Muhammad Asad Naseer, Haiyu Zhou, Weidong Cheng, Hexia Xie, Lanqiu Qin, Xiang Yang, Yufeng Jiang, and Xunbo Zhou. 2024. "Screening and Physiological Responses of Maize Inbred Lines to Drought Stress in South China" Sustainability 16, no. 17: 7366. https://doi.org/10.3390/su16177366