Next Article in Journal
Influence of Parental Healthy-Eating Attitudes and Nutritional Knowledge on Nutritional Adequacy and Diet Quality among Preschoolers: The SENDO Project
Next Article in Special Issue
Proteomic Analysis of Zn Depletion/Repletion in the Hormone-Secreting Thyroid Follicular Cell Line FRTL-5
Previous Article in Journal
New Protocol for Production of Reduced-Gluten Wheat Bread and Pasta and Clinical Effect in Patients with Irritable Bowel Syndrome: A randomised, Double-Blind, Cross-Over Study
Previous Article in Special Issue
Fe, Zn and Se Bioavailability in Chicken Meat Emulsions Enriched with Minerals, Hydroxytyrosol and Extra Virgin Olive Oil as Measured by Caco-2 Cell Model
 
 
Search for Articles:
Title / Keyword
Author / Affiliation / Email
Journal
Article Type
 
 
Advanced Search
 
Section
Special Issue
Volume
Issue
Number
Page
 
Journals
Nutrients
Volume 10
Issue 12
10.3390/nu10121874
nutrients-logo
Submit to this Journal Review for this Journal Propose a Special Issue
► Article Menu

Article Menu

share Share announcement Help format_quote Cite question_answer Discuss in SciProfiles
first_page
settings
Order Article Reprints
Font Type:
Arial Georgia Verdana
Font Size:
Aa Aa Aa
Line Spacing:
Column Width:
Background:
Article

The Zinc-Metallothionein Redox System Reduces Oxidative Stress in Retinal Pigment Epithelial Cells

by
Sara Rodríguez-Menéndez
1,2,
Montserrat García
1,3,*,
Beatriz Fernández
1,2,*,
Lydia Álvarez
1,
Andrés Fernández-Vega-Cueto
1,
Miguel Coca-Prados
1,4,
Rosario Pereiro
1,2 and
Héctor González-Iglesias
1,2,3
1
Instituto Universitario Fernández-Vega (Fundación de Investigación Oftalmológica, Universidad de Oviedo), 33012 Oviedo, Spain
2
Department of Physical and Analytical Chemistry, Faculty of Chemistry, University of Oviedo, Julián Clavería, 8, 33006 Oviedo, Spain
3
Instituto Oftalmológico Fernández-Vega, Avda. Dres. Fernández-Vega, 34, 33012 Oviedo, Spain
4
Department of Ophthalmology and Visual Sciences, Yale University School of Medicine, 300 George St, 8100A, New Haven, CT 06510, USA
*
Authors to whom correspondence should be addressed.
Nutrients 2018, 10(12), 1874; https://doi.org/10.3390/nu10121874
Submission received: 6 November 2018 / Revised: 22 November 2018 / Accepted: 27 November 2018 / Published: 2 December 2018
(This article belongs to the Special Issue Health Effects of Dietary Zinc)
Browse Figures
Versions Notes

Abstract

Oxidative stress affects all the structures of the human eye, particularly the retina and its retinal pigment epithelium (RPE). The RPE limits oxidative damage by several protective mechanisms, including the non-enzymatic antioxidant system zinc-metallothionein (Zn-MT). This work aimed to investigate the role of Zn-MT in the protection of RPE from the oxidative damage of reactive oxygen intermediates by analytical and biochemical-based techniques. The Zn-MT system was induced in an in vitro model of RPE cells and determined by elemental mass spectrometry with enriched isotopes and mathematical calculations. Induced-oxidative stress was quantified using fluorescent probes. We observed that 25, 50 or 100 μM of zinc induced Zn-MT synthesis (1.6-, 3.6- and 11.9-fold, respectively), while pre-treated cells with zinc (25, 50, and 100 μM) and subsequent 2,2′-Azobis(2-methylpropionamidine) dihydrochloride (AAPH) treatment increased Zn-MT levels in a lesser extent (0.8-, 2.1-, 6.1-fold, respectively), exerting a stoichiometric transition in the Zn-MT complex. Moreover, AAPH treatment decreased MT levels (0.4-fold), while the stoichiometry remained constant or slightly higher when compared to non-treated cells. Convincingly, induction of Zn-MT significantly attenuated oxidative stress produced by free radicals’ generators. We conclude that the stoichiometry of Zn-MT plays an important role in oxidative stress response, related with cellular metal homeostasis.
Keywords: zinc; supplementation; metallothioneins; Zn-MT stoichiometry; retinal pigment epithelial cells; oxidative stress; mass-spectrometry zinc; supplementation; metallothioneins; Zn-MT stoichiometry; retinal pigment epithelial cells; oxidative stress; mass-spectrometry
Graphical Abstract

Share and Cite

MDPI and ACS Style

Rodríguez-Menéndez, S.; García, M.; Fernández, B.; Álvarez, L.; Fernández-Vega-Cueto, A.; Coca-Prados, M.; Pereiro, R.; González-Iglesias, H. The Zinc-Metallothionein Redox System Reduces Oxidative Stress in Retinal Pigment Epithelial Cells. Nutrients 2018, 10, 1874. https://doi.org/10.3390/nu10121874

AMA Style

Rodríguez-Menéndez S, García M, Fernández B, Álvarez L, Fernández-Vega-Cueto A, Coca-Prados M, Pereiro R, González-Iglesias H. The Zinc-Metallothionein Redox System Reduces Oxidative Stress in Retinal Pigment Epithelial Cells. Nutrients. 2018; 10(12):1874. https://doi.org/10.3390/nu10121874

Chicago/Turabian Style

Rodríguez-Menéndez, Sara, Montserrat García, Beatriz Fernández, Lydia Álvarez, Andrés Fernández-Vega-Cueto, Miguel Coca-Prados, Rosario Pereiro, and Héctor González-Iglesias. 2018. "The Zinc-Metallothionein Redox System Reduces Oxidative Stress in Retinal Pigment Epithelial Cells" Nutrients 10, no. 12: 1874. https://doi.org/10.3390/nu10121874

APA Style

Rodríguez-Menéndez, S., García, M., Fernández, B., Álvarez, L., Fernández-Vega-Cueto, A., Coca-Prados, M., Pereiro, R., & González-Iglesias, H. (2018). The Zinc-Metallothionein Redox System Reduces Oxidative Stress in Retinal Pigment Epithelial Cells. Nutrients, 10(12), 1874. https://doi.org/10.3390/nu10121874

Note that from the first issue of 2016, this journal uses article numbers instead of page numbers. See further details here.

Article Metrics

Back to TopTop