Next Article in Journal
Prevalence and Correlates of Sarcopenia among Elderly CKD Outpatients on Tertiary Care
Previous Article in Journal
Iron Supplements Containing Lactobacillus plantarum 299v Increase Ferric Iron and Up-regulate the Ferric Reductase DCYTB in Human Caco-2/HT29 MTX Co-Cultures
 
 
Search for Articles:
Title / Keyword
Author / Affiliation / Email
Journal
Article Type
 
 
Advanced Search
 
Section
Special Issue
Volume
Issue
Number
Page
 
Journals
Nutrients
Volume 10
Issue 12
10.3390/nu10121950
nutrients-logo
Submit to this Journal Review for this Journal Propose a Special Issue
► Article Menu

Article Menu

share Share announcement Help format_quote Cite question_answer Discuss in SciProfiles
first_page
settings
Order Article Reprints
Font Type:
Arial Georgia Verdana
Font Size:
Aa Aa Aa
Line Spacing:
Column Width:
Background:
Article

Oleuropein, the Main Polyphenol of Olea europaea Leaf Extract, Has an Anti-Cancer Effect on Human BRAF Melanoma Cells and Potentiates the Cytotoxicity of Current Chemotherapies

by
Jessica Ruzzolini
1,
Silvia Peppicelli
1,
Elena Andreucci
1,
Francesca Bianchini
1,
Arianna Scardigli
2,
Annalisa Romani
2,
Giancarlo La Marca
3,
Chiara Nediani
1,* and
Lido Calorini
1,4,*
1
Department of Experimental and Clinical Biomedical Sciences “Mario Serio”, University of Florence, 50134 Florence, Italy
2
PHYTOLAB (Pharmaceutical, Cosmetic, Food Supplement Technology and Analysis)-DiSIA, Scientific and Technological Pole, University of Florence, 50134 Florence, Italy
3
Mass Spectrometry Laboratory, Clinic of Pediatric Neurology, Meyer University Children’s Hospital, 50139 Florence, Italy
4
Istituto Toscano Tumori and Center of Excellence for Research, Transfer and High Education DenoTHE University of Florence, 50139 Florence, Italy
*
Authors to whom correspondence should be addressed.
Nutrients 2018, 10(12), 1950; https://doi.org/10.3390/nu10121950
Submission received: 9 November 2018 / Revised: 30 November 2018 / Accepted: 5 December 2018 / Published: 8 December 2018
Browse Figures
Versions Notes

Abstract

Oleuropein (Ole), a secoiridoid glucoside present in Olea europaea leaves, gained scientific interest thanks to its several biological properties, including the anticancer one. We verified whether Ole might potentiate the cytotoxicity of conventional drugs used to treat melanoma, disclosing a potentially new therapeutic strategy. We tested the cytotoxic action of Ole alone or in combination with chemotherapeutics on A375 human melanoma cells. We found that Ole was able, at a dose of 500 µM, to stimulate apoptosis, while at a non-toxic dose of 250 µM, it affected cell proliferation and induced the downregulation of the pAKT/pS6 pathway. A dose of 250 µM Ole did not potentiate the effect of Vemurafenib (PLX4032), but it succeeded in increasing the cytotoxic effect of Dacarbazine (DTIC). The major effect was found in the association between Ole and Everolimus (RAD001), also on PLX4032-resistant BRAF melanoma cells, which possibly cooperate in the inhibition of the pAKT/pS6 pathway. Of interest, an olive leaf extract enriched in equimolar Ole was more effective and able to further improve DTIC and RAD001 efficacy on BRAF melanoma cells with respect to Ole alone. Therefore, Ole represents a natural product able to potentiate a wide array of chemotherapeutics against BRAF melanoma cells affecting the pAKT/pS6 pathway.
Keywords: BRAF melanoma; chemotherapeutics; extra virgin oil; Oleuropein; olive leaf extract BRAF melanoma; chemotherapeutics; extra virgin oil; Oleuropein; olive leaf extract

Share and Cite

MDPI and ACS Style

Ruzzolini, J.; Peppicelli, S.; Andreucci, E.; Bianchini, F.; Scardigli, A.; Romani, A.; La Marca, G.; Nediani, C.; Calorini, L. Oleuropein, the Main Polyphenol of Olea europaea Leaf Extract, Has an Anti-Cancer Effect on Human BRAF Melanoma Cells and Potentiates the Cytotoxicity of Current Chemotherapies. Nutrients 2018, 10, 1950. https://doi.org/10.3390/nu10121950

AMA Style

Ruzzolini J, Peppicelli S, Andreucci E, Bianchini F, Scardigli A, Romani A, La Marca G, Nediani C, Calorini L. Oleuropein, the Main Polyphenol of Olea europaea Leaf Extract, Has an Anti-Cancer Effect on Human BRAF Melanoma Cells and Potentiates the Cytotoxicity of Current Chemotherapies. Nutrients. 2018; 10(12):1950. https://doi.org/10.3390/nu10121950

Chicago/Turabian Style

Ruzzolini, Jessica, Silvia Peppicelli, Elena Andreucci, Francesca Bianchini, Arianna Scardigli, Annalisa Romani, Giancarlo La Marca, Chiara Nediani, and Lido Calorini. 2018. "Oleuropein, the Main Polyphenol of Olea europaea Leaf Extract, Has an Anti-Cancer Effect on Human BRAF Melanoma Cells and Potentiates the Cytotoxicity of Current Chemotherapies" Nutrients 10, no. 12: 1950. https://doi.org/10.3390/nu10121950

APA Style

Ruzzolini, J., Peppicelli, S., Andreucci, E., Bianchini, F., Scardigli, A., Romani, A., La Marca, G., Nediani, C., & Calorini, L. (2018). Oleuropein, the Main Polyphenol of Olea europaea Leaf Extract, Has an Anti-Cancer Effect on Human BRAF Melanoma Cells and Potentiates the Cytotoxicity of Current Chemotherapies. Nutrients, 10(12), 1950. https://doi.org/10.3390/nu10121950

Note that from the first issue of 2016, this journal uses article numbers instead of page numbers. See further details here.

Article Metrics

Back to TopTop