Preserved Skeletal Muscle Mitochondrial Function, Redox State, Inflammation and Mass in Obese Mice with Chronic Heart Failure
Abstract
:1. Introduction
2. Methods
2.1. Study Protocol
2.2. Blood Glucose
2.3. Mitochondrial Function
2.4. Superoxide Generation and Redox State
2.5. Protein Expression and Phosphorylation
2.6. Statistical Analysis
3. Results
3.1. Calorie Intake, Body and Tissue Weight and Plasma Glucose
3.2. Skeletal Muscle Mitochondrial ATP and ROS Production
3.3. Skeletal Muscle Cytokine Profile
3.4. Skeletal Muscle Insulin Signalling
4. Discussion
Author Contributions
Funding
Acknowledgments
Conflicts of Interest
References
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Sham | LCHF | ObCHF | ||
---|---|---|---|---|
Body Weight [g] | T0 | 42.0 ± 1.5 a | 42.4 ± 0.9 a | 41.9 ± 1.6 a |
Body Weight [g] | T12 | 44.1 ± 1.2 a | 43.1 ± 1.3 a | 53.0 ± 0.9 b,* |
Body Weight [g] | T20 | 46.9 ± 1.3 a,* | 43.9 ± 1.1 b | 53.6 ± 1.4 c,* |
Average daily caloric intake (kcal/d) | T0-T12 | 7.7 ± 0.6 a | 7.8 ± 0.3 a | 25.5 ± 4.1 b |
Average daily caloric intake (kcal/d) | T13-T20 | 7.7 ± 0.9 a | 8.1 ± 0.4 a | 8.9 ± 2.1 a,* |
Left Retroperitoneal Fat Pad mass [mg] | T20 | 294 ± 70 a | 249 ± 58 b | 493 ± 72 c |
Left Epididimal Fat Pad mass [mg] | T20 | 1031 ± 161 a | 675 ± 162 b | 1351 ± 126 c |
Plasma Glucose [mg/dl] | T20 | 131 ± 4 a | 140 ± 3 a | 155 ± 3 b |
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Gortan Cappellari, G.; Aleksova, A.; Dal Ferro, M.; Cannatà, A.; Semolic, A.; Zanetti, M.; Springer, J.; Anker, S.D.; Giacca, M.; Sinagra, G.; et al. Preserved Skeletal Muscle Mitochondrial Function, Redox State, Inflammation and Mass in Obese Mice with Chronic Heart Failure. Nutrients 2020, 12, 3393. https://doi.org/10.3390/nu12113393
Gortan Cappellari G, Aleksova A, Dal Ferro M, Cannatà A, Semolic A, Zanetti M, Springer J, Anker SD, Giacca M, Sinagra G, et al. Preserved Skeletal Muscle Mitochondrial Function, Redox State, Inflammation and Mass in Obese Mice with Chronic Heart Failure. Nutrients. 2020; 12(11):3393. https://doi.org/10.3390/nu12113393
Chicago/Turabian StyleGortan Cappellari, Gianluca, Aneta Aleksova, Matteo Dal Ferro, Antonio Cannatà, Annamaria Semolic, Michela Zanetti, Jochen Springer, Stefan D. Anker, Mauro Giacca, Gianfranco Sinagra, and et al. 2020. "Preserved Skeletal Muscle Mitochondrial Function, Redox State, Inflammation and Mass in Obese Mice with Chronic Heart Failure" Nutrients 12, no. 11: 3393. https://doi.org/10.3390/nu12113393