Sedum takesimense Protects PC12 Cells against Corticosterone-Induced Neurotoxicity by Inhibiting Neural Apoptosis
Abstract
:1. Introduction
2. Materials and Methods
2.1. Preparation of WEST
2.2. Cell Culture and Treatment
2.3. Cell Viability Assay
2.4. LDH Leakage Assay
2.5. Hoechst 33342 and PI Double Staining
2.6. TUNEL Staining
2.7. Intracellular ROS Level Assay
2.8. Intracellular Ca2+ Level Assay
2.9. Detection of mPTP Opening
2.10. Measurement of MMP
2.11. ATP Detection Assay
2.12. Western Blot Analysis
2.13. Statistical Analysis
3. Results
3.1. WEST Alleviates Corticosterone-Induced Injury in PC12 Cells
3.2. WEST Inhibits Apoptosis Induced by Corticosterone in PC12 Cells
3.3. WEST Prevents Corticosterone-Induced ER Stress in PC12 Cells
3.4. WEST Protects PC12 Cells against Mitochondrial Dysfunction Following Cell Damage
3.5. WEST Modulates the Expression of Apoptosis-Related Proteins in Corticosterone-Treated PC12 Cells
4. Discussion
Supplementary Materials
Author Contributions
Funding
Conflicts of Interest
References
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Compounds | |
---|---|
ferulic acid caffeic acid gallic acid methyl gallate myricetin quercetin luteolin rhodalin | rhodalidin arbutin 2,6-di-O-galloylarbutin luteolin-7-O-β-D-glucoside gossypetin-8-O-β-D-xylopyranoside 1-(4-hydroxyphenyl)-2-(3,5-dihydroxyphenyl)-2-hydroxyethanone |
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Yun, H.-Y.; Jeong, Y. Sedum takesimense Protects PC12 Cells against Corticosterone-Induced Neurotoxicity by Inhibiting Neural Apoptosis. Nutrients 2020, 12, 3713. https://doi.org/10.3390/nu12123713
Yun H-Y, Jeong Y. Sedum takesimense Protects PC12 Cells against Corticosterone-Induced Neurotoxicity by Inhibiting Neural Apoptosis. Nutrients. 2020; 12(12):3713. https://doi.org/10.3390/nu12123713
Chicago/Turabian StyleYun, Hea-Yeon, and Yoonhwa Jeong. 2020. "Sedum takesimense Protects PC12 Cells against Corticosterone-Induced Neurotoxicity by Inhibiting Neural Apoptosis" Nutrients 12, no. 12: 3713. https://doi.org/10.3390/nu12123713