The Effect of Sheep and Cow Milk Supplementation of a Low Calcium Diet on the Distribution of Macro and Trace Minerals in the Organs of Weanling Rats
Abstract
:1. Introduction
2. Materials and Methods
2.1. Animals
2.2. Diets and Procedures
2.3. Proximate Analysis of Diets and Milk
2.4. Analysis of Mineral Composition
2.5. Statistical Analysis
3. Results and Discussion
3.1. Diet Composition
3.2. Rat Food and Milk Intake
3.3. Rat Weight Gain
3.4. Macro and Trace Minerals in Soft Organs and Serum
3.5. Non-Essential Minerals in Soft Organs and Serum
4. Conclusions
Supplementary Materials
Author Contributions
Funding
Acknowledgments
Conflicts of Interest
References
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Diet | Modified-AIN-93M (g) | Low Ca/P modified-AIN-93M (g) |
---|---|---|
Ingredient | ||
Beef protein extract | 140 | 140 |
L-cystine | 1.8 | 1.8 |
Corn starch | 495.69 | 495.69 |
Maltodextrin | 125 | 125 |
Sucrose | 106.69 | 106.69 |
Cellulose | 50 | 50 |
Soybean oil | 40 | 40 |
t-butylhydroquinone | 0.008 | 0.008 |
Mineral mix a | 3.5 | 3.5 |
NaCl | 2.59 | 2.59 |
CaCO3 b | 12.495 | 6.248 |
KH2PO4 c | 8.75 | 4.375 |
K3C6H5O d | 0.98 | 4.375 |
Vitamin mix e | 10 | 10 |
Choline bitartrate | 2.5 | 2.5 |
Yellow dye f | 0.00 | 0.05 |
Fraction | Basal Diet * | Milk # | ||
---|---|---|---|---|
Modified-AIN-93M (%) | Low Ca/P Modified-AIN-93M (%) | Cow Milk (%) | Sheep Milk (%) | |
Protein | 14 | 14 | 3.69 | 5.55 |
Lipid | 4 | 4 | 3.63^ | 8.72 ^ |
Carbohydrate | 76 | 76 | - | - |
Lactose | - | - | 4.81 | 4.47 |
Diet | Control [µg/day] | Low Ca/P Control [µg/day] | Low Ca/P + CM [µg/day] | Low Ca/P + SM [µg/day] | |
---|---|---|---|---|---|
Mineral Type | Mineral Intake | ||||
Macro | Ca * | 75.4 ± 7.17 b | 53.8 ± 3.75 c | 93.3 ± 5.44 a | 97.4 ± 11.7 a |
Mg * | 14.8 ± 1.40 ab | 14.0 ± 0.98 b | 15.8 ± 0.73 a | 15.4 ± 1.38 a | |
K * | 180 ± 17.1 b | 168 ± 11.7 bc | 198 ± 9.01 a | 160 ± 13.5 c | |
P * | 56.1 ± 5.33 b | 42.7 ± 2.98 c | 76.7 ± 4.61 a | 79.5 ± 9.68 a | |
Na * | 123 ± 11.7 a | 113 ± 7.85 b | 108 ± 5.89 b | 113 ± 9.65 b | |
Trace | Cu | 148 ± 14.1 a | 117 ± 8.17 b | 97.8 ± 6.80 c | 96.9 ± 8.02 c |
Fe | 180 ± 17.1 a | 168 ± 11.7 a | 148 ± 9.30 b | 170 ± 14.6 a | |
Mn | 262 ± 24.9 a | 220 ± 15.4 b | 182 ± 12.9 c | 165 ± 14.3 c | |
Mo | 3.63 ± 0.35 a | 2.66 ± 0.19 b | 2.18 ± 0.16 c | 1.94 ± 0.17 d | |
Ni | 14.0 ± 1.33 a | 12.3 ± 0.86 b | 10.1 ± 0.72 c | 9.07 ± 0.79 c | |
Zn * | 1.03 ± 0.10 a | 0.93 ± 0.07 b | 0.91 ± 0.06 bc | 0.84 ± 0.07 c | |
Non-essential | Al | 220 ± 21.0 a | 240 ± 16.8 a | 197 ± 14.1 b | 234 ± 19.8 a |
Ce # | 390 ± 37.1 a | 303 ± 21.1 b | 249 ± 17.8 c | 221 ± 19.5 d | |
Cr | 36.5 ± 3.47 a | 31.0 ± 2.16 b | 25.5 ± 1.83 c | 22.6 ± 1.99 d | |
Cs | 0.37 ± 0.04 b | 0.35 ± 0.02 b | 0.33 ± 0.02 b | 1.13 ± 0.17 a | |
Er # | 32.7 ± 3.11 a | 30.4 ± 2.12 a | 25.0 ± 1.79 b | 22.1 ± 1.95 c | |
La | 2.77 ± 0.26 a | 2.56 ± 0.18 b | 2.11 ± 0.15 c | 1.87 ± 0.16 d | |
Li | 2.77 ± 0.26 a | 2.56 ± 0.18 a | 2.11 ± 0.15 b | 2.17 ± 0.18 b | |
Nd # | 215 ± 20.5 a | 185 ± 12.9 b | 152 ± 10.9 c | 135 ± 11.9 d | |
Ni | 14.0 ± 1.33 a | 12.3 ± 0.86 b | 10.1 ± 0.72 c | 9.07 ± 0.79 c | |
Rb | 72.2 ± 6.86 c | 67.2 ± 4.69 c | 131 ± 8.4 a | 110 ± 12.6 b | |
Sr | 41.9 ± 3.98 b | 31.6 ± 2.20 c | 46.9 ± 2.38 b | 75.1 ± 10.1 a | |
U | 240 ± 22.9 a | 153 ± 10.6 b | 125 ± 8.99 c | 111 ± 9.81 c | |
V | 12.0 ± 1.12 a | 10.0 ± 0.73 b | 9.00 ± 0.62 c | 8.00 ± 0.67 d | |
Y # | 511 ± 48.5 a | 453 ± 31.6 a | 373 ± 26.7 b | 331 ± 29.1 c |
Diet | Control | Low Ca/P Control | Low Ca/P + CM | Low Ca/P + SM | ||
---|---|---|---|---|---|---|
Organ | Element Type | Element | [µg/kg] | [µg/kg] | [µg/kg] | [µg/kg] |
Brain | Macro | Ca # | 46.3 ± 3.79 | 46.9 ± 2.70 | 46.5 ± 2.92 | 45.9 ± 3.32 |
K @ | 3.64 ± 0.18 | 3.76 ± 0.20 | 3.73 ± 0.16 | 3.66 ± 0.20 | ||
Mg # | 148 ± 4.40 | 151 ± 5.77 | 150 ± 5.03 | 149 ± 5.16 | ||
Na # | 1.07 ± 0.04 | 1.10 ± 0.07 | 1.11 ± 0.04 | 1.10 ± 0.05 | ||
P @ | 2.88 ±0.10 | 2.93 ± 0.14 | 2.96 ± 0.14 | 2.95 ± 0.11 | ||
Trace | Cu # | 1.85 ± 0.10 | 1.83 ± 0.10 | 1.85 ± 0.10 | 1.91 ± 0.11 | |
Fe # | 17.9 ± 2.14 | 17.5 ± 1.79 | 17.2 ± 2.48 | 18.2 ± 2.76 | ||
Mn | 353 ± 35.2 | 373 ± 22.9 | 370 ± 16.5 | 364 ± 17.5 | ||
Mo | 26.2 ± 2.67 | 25.6 ± 2.39 | 26.1 ± 1.82 | 25.1 ± 1.79 | ||
Zn # | 11.7 ± 0.46 | 11.9 ± 0.49 | 11.9 ± 0.43 | 11.6 ± 0.52 | ||
Non-essential | As | 22.2 ± 4.16 a | 20.7 ± 2.84 ab | 15.1 ± 2.23 c | 16.3 ± 3.12 bc | |
Co | 3.89 ± 0.42 | 4.06 ± 0.82 | 3.41 ± 0.41 | 3.47 ± 0.44 | ||
Cs | 5.20 ± 0.79 b | 4.87 ± 0.44 b | 4.61 ± 0.48 b | 9.38 ± 1.74 a | ||
Rb # | 1.29 ± 0.13 b | 1.35 ± 0.17 b | 2.09 ± 0.22 a | 2.05 ± 0.27 a | ||
Sr | 10.7 ± 2.39 b | 29.9 ± 17.6 a | 13.8 ± 6.16 b | 17.8 ± 8.97 ab | ||
Pb | BDL | BDL | BDL | BDL | ||
Kidney | Macro | Ca # | 69.5 ± 8.41 | 69.2 ± 4.97 | 67.9 ± 6.03 | 69.0 ± 5.25 |
K @ | 2.49 ± 0.38 | 2.71 ± 0.15 | 2.54 ± 0.28 | 2.55 ± 0.19 | ||
Mg # | 180 ± 26.2 | 185 ± 9.28 | 178 ± 17.3 | 179 ± 13.2 | ||
Na # | 1.42 ± 0.15 | 1.42 ± 0.07 | 1.40 ± 0.10 | 1.46 ± 0.14 | ||
P @ | 2.61 ± 0.40 | 2.62 ± 0.11 | 2.54 ± 0.26 | 2.54 ± 0.21 | ||
Trace | Cu # | 3.88 ± 0.54 b | 3.38 ± 0.20 b | 5.29 ± 1.13 a | 5.34 ± 1.01 a | |
Fe # | 60.0 ± 10.4 | 57.7 ± 6.58 | 59.5 ± 7.94 | 61.9 ± 10.6 | ||
Mn | 750 ± 123 | 829 ± 68.8 | 755 ± 105 | 764 ± 78.6 | ||
Mo | 180 ± 25.2 | 184 ± 15.6 | 183 ± 23.6 | 187 ± 16.7 | ||
Zn # | 18.0 ± 2.14 | 18.1 ± 1.98 | 18.0 ± 2.00 | 18.5 ± 1.46 | ||
Non-essential | As | 75.8 ± 19.1 ab | 65.1 ± 6.12 a | 56.6 ± 10.7 ab | 61.0 ± 14.5 b | |
Co | 85.4 ± 17.5 | 83.3 ± 11.4 | 68.9 ± 9.89 | 66.1 ± 9.26 | ||
Cs | 14.0 ± 2.95 b | 13.8 ± 1.62 b | 12.0 ± 1.37 b | 20.2 ± 2.69 a | ||
Rb # | 2.92 ± 0.51 b | 3.54 ± 0.37 b | 4.96 ± 0.67 a | 4.76 ± 0.66 a | ||
Sr | 24.1 ± 4.70 b | 46.7 ± 5.22 a | 27.6 ± 5.37 b | 39.4 ± 8.14 a | ||
Pb | 51.7 ± 38.8 | 81.5 ± 48.7 | 55.2 ± 59.0 | 105 ± 76.1 | ||
Liver | Macro | Ca # | 33.7 ± 3.56 | 32.8 ± 3.17 | 35.4 ± 1.91 | 36.4 ± 3.35 |
K @ | 3.34 ± 0.27 b | 3.41 ± 0.10 b | 3.61 ± 0.14 a | 3.55 ± 0.19 ab | ||
Mg # | 201 ± 17.6 | 206 ± 7.91 | 209 ± 9.70 | 209 ± 14.4 | ||
Na # | 668 ± 45.0 | 686 ± 37.9 | 663 ± 46.4 | 690 ± 49.6 | ||
P @ | 2.90 ± 0.21 | 2.93 ± 0.14 | 3.00 ± 0.18 | 3.04 ± 0.22 | ||
Trace | Cu # | 3.08 ± 0.34 b | 3.12 ± 0.13 b | 3.34 ± 0.24 ab | 3.41 ± 0.19 a | |
Fe # | 128 ± 22.8 a | 138 ± 16.9 a | 89.1 ± 9.79 b | 88.8 ± 10.6 b | ||
Mn | 1.41 ± 0.16 b | 1.57 ± 0.15 ab | 1.72 ± 0.15 a | 1.79 ± 0.11 a | ||
Mo | 235 ± 23.9 b | 214 ± 29.5 b | 297 ± 30.7 a | 335 ± 37.9 a | ||
Zn # | 20.8 ± 1.75 b | 21.0 ± 1.82 b | 24.0 ± 1.97 a | 25.0 ± 2.06 a | ||
Non-essential | As | 57.1 ± 13.4 ab | 64.3 ± 11.4 a | 45.8 ± 11.6 b | 47.7 ± 12.4 ab | |
Co | 15.6 ± 1.70 ab | 18.1 ± 2.88 a | 12.1 ± 2.81 c | 12.3 ± 2.44 bc | ||
Cs | 8.80 ± 1.05 b | 8.49 ± 0.91 b | 8.23 ± 0.80 b | 15.6 ± 1.88 a | ||
Rb # | 4.84 ± 0.42 b | 4.98 ± 0.54 b | 8.51 ± 0.82 a | 8.38 ± 0.92 a | ||
Sr | 11.6 ± 1.56 c | 17.0 ± 2.07 a | 13.0 ± 1.92 bc | 15.1 ± 1.44 ab | ||
Pb | BDL | BDL | BDL | BDL | ||
Spleen | Macro | Ca # | 31.2 ± 5.81 | 36.6 ± 4.88 | 34.2 ± 7.04 | 37.3 ± 3.69 |
K @ | 4.12 ± 0.54 | 4.54 ± 0.36 | 4.35 ± 0.53 | 4.47 ± 0.50 | ||
Mg # | 187 ± 25.0 | 207 ± 17.7 | 199 ± 24.8 | 206 ± 22.7 | ||
Na # | 490 ± 71.4 b | 559 ± 65.0 a | 551 ± 41.8 ab | 545 ± 52.5 ab | ||
P @ | 3.05 ± 0.45 | 3.39 ± 0.32 | 3.35 ± 0.15 | 3.39 ± 0.43 | ||
Trace | Cu # | 0.94 ± 0.16 | 0.98 ± 0.09 | 0.92 ± 0.19 | 1.00 ± 0.12 | |
Fe # | 305 ± 55.7 ab | 427 ± 99.1 a | 238 ± 75.0 bc | 205 ± 41.5 c | ||
Mn | 150 ± 25.2 | 178 ± 20.0 | 154 ± 28.6 | 162 ± 18.0 | ||
Mo | 53.2 ± 14.9 ab | 66.1 ± 11 a | 50.4 ± 14.9 b | 55.4 ± 12.4 ab | ||
Zn # | 16.2 ± 2.28 | 18.2 ± 1.94 | 17.3 ± 1.96 | 18.0 ± 2.03 | ||
Non-essential | As | 171 ± 45.7 a | 197 ± 37.7 a | 116 ± 31.8 b | 115 ± 16.5 b | |
Co | 8.78 ± 1.10 a | 10.1 ± 1.71 a | 5.89 ± 1.64 b | 5.59 ± 0.85 b | ||
Cs | 8.01 ± 2.11 b | 8.22 ± 0.94 b | 6.52 ± 1.26 b | 12.3 ± 1.57 a | ||
Rb # | 3.15 ± 0.40 c | 3.73 ± 0.44 bc | 5.05 ± 1.13 ab | 5.43 ± 0.80 a | ||
Sr | 10.3 ± 1.42 c | 19.5 ± 3.24 a | 11.8 ± 2.21 b | 17.2 ± 2.92 a | ||
Pb | BDL | BDL | BDL | BDL |
Diet | Control | Low Ca/P Control | Low Ca/P + CM | Low Ca/P + SM | |
---|---|---|---|---|---|
Element Type | Element | [µg/mL] | [µg/mL] | [µg/mL] | [µg/mL] |
Macro | Ca | 142 ± 10.8 | 140 ± 8.73 | 141 ± 8.06 | 138 ± 10.2 |
K | 331 ± 44.1 | 284 ± 54.3 | 306 ± 46.5 | 281 ± 45.3 | |
Mg | 29.1 ± 6.30 | 28.6 ± 4.46 | 26.5 ± 3.72 | 29.9 ± 7.20 | |
P | 199 ± 18.1 | 181 ± 12.5 | 187 ± 19.3 | 186 ± 9.71 | |
Trace | Cu | 1.00 ± 0.12 | 0.94 ± 0.05 | 1.00 ± 0.10 | 1.01 ± 0.09 |
Fe | 3.41 ± 0.91 | 3.07 ± 1.37 | 4.20 ± 1.15 | 4.11 ± 1.17 | |
Zn | 1.57 ± 0.15 | 1.55 ± 0.17 | 1.65 ± 0.25 | 1.70 ± 0.26 | |
Non-essential | Cs * | 0.88 ± 0.23ab | 0.67 ± 0.12b | 0.66 ± 0.14b | 1.00 ± 0.17a |
Rb | 0.28 ± 0.06ab | 0.22 ± 0.05b | 0.37 ± 0.09a | 0.37 ± 0.09a | |
Sr | BDL | BDL | BDL | BDL | |
Pb | BDL | BDL | BDL | BDL |
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Burrow, K.; Young, W.; McConnell, M.; Carne, A.; Barr, D.; Reid, M.; Bekhit, A.E.-D. The Effect of Sheep and Cow Milk Supplementation of a Low Calcium Diet on the Distribution of Macro and Trace Minerals in the Organs of Weanling Rats. Nutrients 2020, 12, 594. https://doi.org/10.3390/nu12030594
Burrow K, Young W, McConnell M, Carne A, Barr D, Reid M, Bekhit AE-D. The Effect of Sheep and Cow Milk Supplementation of a Low Calcium Diet on the Distribution of Macro and Trace Minerals in the Organs of Weanling Rats. Nutrients. 2020; 12(3):594. https://doi.org/10.3390/nu12030594
Chicago/Turabian StyleBurrow, Keegan, Wayne Young, Michelle McConnell, Alan Carne, David Barr, Malcolm Reid, and Alaa El-Din Bekhit. 2020. "The Effect of Sheep and Cow Milk Supplementation of a Low Calcium Diet on the Distribution of Macro and Trace Minerals in the Organs of Weanling Rats" Nutrients 12, no. 3: 594. https://doi.org/10.3390/nu12030594