Selenoneine Ameliorates Hepatocellular Injury and Hepatic Steatosis in a Mouse Model of NAFLD

Miyata, Masaaki; Matsushita, Koki; Shindo, Ryunosuke; Shimokawa, Yutaro; Sugiura, Yoshimasa; Yamashita, Michiaki

doi:10.3390/nu12061898

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Selenoneine Ameliorates Hepatocellular Injury and Hepatic Steatosis in a Mouse Model of NAFLD

by

Masaaki Miyata

^*,

Koki Matsushita

,

Ryunosuke Shindo

,

Yutaro Shimokawa

,

Yoshimasa Sugiura

and

Michiaki Yamashita

Department of Food Science and Technology, National Research and Development Agency, Japan Fisheries Research and Education Agency, National Fisheries University, 2-7-1, Nagata-Honmachi, Shimonoseki 759-6595, Japan

^*

Author to whom correspondence should be addressed.

Nutrients 2020, 12(6), 1898; https://doi.org/10.3390/nu12061898

Submission received: 9 May 2020 / Revised: 20 June 2020 / Accepted: 23 June 2020 / Published: 26 June 2020

(This article belongs to the Special Issue Dietary Minerals and Human Health)

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Abstract

Selenoneine is a novel organic selenium compound markedly found in the blood, muscles, and other tissues of fish. This study aimed to determine whether selenoneine attenuates hepatocellular injury and hepatic steatosis in a mouse model of non-alcoholic fatty liver disease (NAFLD). Mice lacking farnesoid X receptor (FXR) were used as a model for fatty liver disease, because they exhibited hepatomegaly, hepatic steatosis, and hepatic inflammation. Fxr-null mice were fed a 0.3 mg Se/kg selenoneine-containing diet for four months. Significant decreases in the levels of hepatomegaly, hepatic damage-associated diagnostic markers, hepatic triglycerides, and total bile acids were found in Fxr-null mice fed with a selenoneine-rich diet. Hepatic and blood clot total selenium concentrations were 1.7 and 1.9 times higher in the selenoneine group than in the control group. A marked accumulation of selenoneine was found in the liver and blood clot of the selenoneine group. The expression levels of oxidative stress-related genes (heme oxygenase 1 (Hmox1), glutathione S-transferase alpha 1 (Gsta1), and Gsta2), fatty acid synthetic genes (stearoyl CoA desaturase 1(Scd1) and acetyl-CoA carboxylase 1 (Acc1)), and selenoprotein (glutathione peroxidase 1 (Gpx1) and selenoprotein P (Selenop)) were significantly decreased in the selenoneine group. These results suggest that selenoneine attenuates hepatic steatosis and hepatocellular injury in an NAFLD mouse model.

Keywords: selenoneine; farnesoid X receptor; non-alcoholic fatty liver disease; selenium; steatosis

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MDPI and ACS Style

Miyata, M.; Matsushita, K.; Shindo, R.; Shimokawa, Y.; Sugiura, Y.; Yamashita, M. Selenoneine Ameliorates Hepatocellular Injury and Hepatic Steatosis in a Mouse Model of NAFLD. Nutrients 2020, 12, 1898. https://doi.org/10.3390/nu12061898

AMA Style

Miyata M, Matsushita K, Shindo R, Shimokawa Y, Sugiura Y, Yamashita M. Selenoneine Ameliorates Hepatocellular Injury and Hepatic Steatosis in a Mouse Model of NAFLD. Nutrients. 2020; 12(6):1898. https://doi.org/10.3390/nu12061898

Chicago/Turabian Style

Miyata, Masaaki, Koki Matsushita, Ryunosuke Shindo, Yutaro Shimokawa, Yoshimasa Sugiura, and Michiaki Yamashita. 2020. "Selenoneine Ameliorates Hepatocellular Injury and Hepatic Steatosis in a Mouse Model of NAFLD" Nutrients 12, no. 6: 1898. https://doi.org/10.3390/nu12061898

APA Style

Miyata, M., Matsushita, K., Shindo, R., Shimokawa, Y., Sugiura, Y., & Yamashita, M. (2020). Selenoneine Ameliorates Hepatocellular Injury and Hepatic Steatosis in a Mouse Model of NAFLD. Nutrients, 12(6), 1898. https://doi.org/10.3390/nu12061898

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Selenoneine Ameliorates Hepatocellular Injury and Hepatic Steatosis in a Mouse Model of NAFLD

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