Administration of Lactobacillus reuteri Combined with Clostridium butyricum Attenuates Cisplatin-Induced Renal Damage by Gut Microbiota Reconstitution, Increasing Butyric Acid Production, and Suppressing Renal Inflammation

Round 1

Reviewer 1 Report

Authors showed that Cisplatin-induced nephrotoxicity is associated with gut microbiota disturbance with using rats which impaired by cisplatin, supplemented Lactobacillus reuteri and Clostridium butyricum (LCs). The trail suggested LCs probiotic serves as a mediator of the gut–kidney axis in cisplatin-induced nephrotoxicity to restore the intestinal microbiota composition and suppressing uremic toxin production and enhancing butyrate production. 

Comments 

The study appears to be properly conducted, and the manuscript is clearly written. However, the following points needs to be addressed.

1. Gut-kidney axis is the most important topic of this manuscript, why don't you use a diagram to illustrate this?
2. So many abbreviations there, please clarify them. 
3. L88, DSS?
4. L283, correct font size of (Table 1).
5. L247, describe full of PCoA. This is first PCoA in this manuscript.
6. L440, that is second PCoA.

Author Response

Dear Reviewer 1,

We deeply appreciated your encouragements and suggestions. We have carefully revised our original manuscript and further improvement the quality of our manuscript. The replies and explanations from point to point for all comments and suggestions. The changes are highlighted in blue and given below. We have highlighted all changes of our typo errors by using red font in the revised manuscript.  Please see the attachment.

Sincerely Yours

 

Chu-Chyn Ou

School of Nutrition,

Chung Shan Medical University,

Taichung, Taiwan

Author Response File: Author Response.pdf

Reviewer 2 Report

1. The effects of LCs on the cis plat induced nephro damage has been reviewed. The work has been well explained covering all the required parameters and assays.
2. There are very few minor concerns in the manuscript.
3. The preliminary introduction lines are vague and needs to be changed. (The traditional cancer treatment (chemotherapy) could provide potential cure; however, many limitations such as the adverse effects are identified. Cisplatin is widely used as an effective chemotherapeutic agent for many carcinomas; however, it can lead to adverse side effects on the kidney and gastrointestinal tract,)
4. The authors are suggested to check minor typos in the manuscript.

Author Response

Dear Reviewer 2,

We deeply appreciated your encouragement and suggestions. We have carefully revised our original manuscript and further improvement the quality of our manuscript. The replies and explanations from point to point for all comments and suggestions. The changes are highlighted in blue. Please see the attachment.

Sincerely Yours

 

Chu-Chyn Ou

School of Nutrition,

Chung Shan Medical University,

Taichung, Taiwan

Author Response File: Author Response.docx