Diagnostic and Therapeutic Perspectives Associated to Cobalamin-Dependent Metabolism and Transcobalamins’ Synthesis in Solid Cancers

Round 1

Reviewer 1 Report

This is a well-designed and well-written article concerning on the relationship between the cobalamin-dependent metabolic pathways, the tumor or plasma transcobalamins levels, and their implications in cancer cell growth to formulate relevant hypotheses for future diagnostic and therapeutic perspectives. The article is comprehensive and contains excellently described content about cobalamin-dependent metabolism and transcobalamins synthesis in solid cancers. Moreover, the paper include many figures showed eg metabolic pathaways what facilitates the understanding of the text. I reccommend to accept the article in present form.

Reviewer 2 Report

In this manuscript (review), the authors presented the diagnosis and prognosis values of the cobalamin (including transcobalamins I and II) and vitamin B12 in a number of solid cancerous lesions, along with their potential use in the treatment of cancer. The reports that bring information about possible biomarkers that can be used for cancer detection are very useful since most malignant lesions are having an insidious evolution and usually the late detection is often associated with a reserved/unpredictable or bad prognosis. Since the biomarkers described in this paper could be useful not only for cancer detection but for other tumor-related details (e.g., the metabolic pathways of the malignant lesions, cancer prognosis, therapeutic strategies etc.), knowledge about them is more than welcome. The review mentions the involvement of cobalamin (including TCN1 and TCN2) or vitamin B12 in the evolution only of some solid cancerous lesions, but despite this, out there are not many realible biomarkers for cancer detection and so many tumoral types. Accordingly, the paper is essential in the approached field and is well documented and written, while the results are clearly presented. Good job!