IGF-1 Signaling Regulates Mitochondrial Remodeling during Myogenic Differentiation
Abstract
:1. Introduction
2. Materials and Methods
2.1. Chemicals and Reagents
2.2. Cell Culture
2.3. SiRNA Transfection
2.4. Immunofluostaining
2.5. Western Blotting
2.6. Transmission Electron Microscopy (TEM)
2.7. Quantitative Real-Time PCR (RT-PCR)
2.8. Flow Cytometry
2.9. Statistical Analysis
3. Results
3.1. IGF-1 Signaling Is Essential for Myogenic Differentiation
3.2. Inhibition of IGF-1 Signaling Leads to Mitochondrial Dysfunction and Damage
3.3. IGF-1 Signaling Regulates Mitochondrial Biogenesis during Myogenic Differentiation
3.4. IGF-1 Signaling Regulates Mitophagy during Myogenic Differentiation
3.5. IGF-1 Induces PGC-1α and BNIP3 Accumulation Dose-Dependently during Differentiation
3.6. IGF-1 Alleviates Impaired Myoblast Differentiation Caused by Mitophagy Deficiency
4. Discussion
5. Conclusions
Author Contributions
Funding
Institutional Review Board Statement
Data Availability Statement
Conflicts of Interest
References
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Gene | Primer Sequence |
---|---|
Bcl2l13 | F:5′-ATGGCGTCCTCTACGACTG-3′ |
R:5′-GGTGAGGGACCTTGTTGTTTC-3′ | |
Bnip3 | F:5′-GTTCCAGCCTCCGTCTCTATT-3′ |
R:5′-CCTCAGACAGAGTGCTGTTTTTC-3′ | |
Cox7a1 | F:5′-CAGCGTCATGGTCAGTCTGT-3′ |
R:5′-AGAAAACCGTGTGGCAGAGA-3′ | |
Fundc1 | F:5′-CCCCCTCCCCAAGACTATGAA-3′ |
R:5′-CCACCCATTACAATCTGAGTAGC-3′ | |
Lpl | F:5′-GAAAGGGCTCTGCCTGAGTT-3′ |
R:5′-TAGGGCATCTGAGAGCGAGT-3′ | |
Nd1 | F:5′-CACTATTCGGAGCTTTACG-3′ |
R:5′-TGTTTCTGCTAGGGTTGA-3′ | |
Nix/Bnip3L | F:5′-ATGTCTCACTTAGTCGAGCCG-3′ |
R:5′-CTCATGCTGTGCATCCAGGA-3′ | |
Nrf1 | F:5′-GCACCTTTGGAGAATGTGGT-3′ |
R:5′-CTGAGCCTGGGTCATTTTGT-3′ | |
Ppargc1a | F:5′-GGACATGTGCAGCCAAGACTC-3′ |
R:5′-CACTTCAATCCACCCAGAAAGCT-3′ | |
Phb2 | F:5′- ATCCGTGTTCACCGTGGAAG-3′ |
R:5′-CCCGAATGTCATAGATGATGGG-3′ | |
Pink1 | F:5′-GCTGATCGAGGAGAAGCAG-3′ |
R:5′-GATAATCCTCCAGACGGAAGC-3′ | |
Tfam | F:5′- CCAAAAAGACCTCGTTCAGC-3′ |
R:5′-CTTCAGCCATCTGCTCTTCC-3′ | |
Tfb1m | F:5′-CACCGAGGGCTTGGAATGTT-3′ |
R:5′-TAGAACCCGCAGCTTTCTGG-3′ | |
18S | F:5′-GTAACCCGTTGAACCCCATT-3′ |
R:5′-CCATCCAATCGGTAGTAGCG-3′ |
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Guan, X.; Yan, Q.; Wang, D.; Du, G.; Zhou, J. IGF-1 Signaling Regulates Mitochondrial Remodeling during Myogenic Differentiation. Nutrients 2022, 14, 1249. https://doi.org/10.3390/nu14061249
Guan X, Yan Q, Wang D, Du G, Zhou J. IGF-1 Signaling Regulates Mitochondrial Remodeling during Myogenic Differentiation. Nutrients. 2022; 14(6):1249. https://doi.org/10.3390/nu14061249
Chicago/Turabian StyleGuan, Xin, Qiyang Yan, Dandan Wang, Guocheng Du, and Jingwen Zhou. 2022. "IGF-1 Signaling Regulates Mitochondrial Remodeling during Myogenic Differentiation" Nutrients 14, no. 6: 1249. https://doi.org/10.3390/nu14061249