Mixed Berry Juice and Cellulose Fiber Have Differential Effects on Peripheral Blood Mononuclear Cell Respiration in Overweight Adults
, George P. Albaugh 1, Hawi A. Debelo 3,4, Mario G. Ferruzzi 3,5, David J. Baer 1 and Janet A. Novotny 1,*Round 1
Reviewer 1 Report
These study confirm, berries and other antocyanin-rich foods have demonstrate anti-obesity effects in humans.
The article is a confirmation of the many health benefits of eating berries. There are a variety of chemical compounds present in berries that bring benefits mainly due to their antioxidant activity. Many studies highlight the health benefits of these compounds. The authors have presented this very well. Blueberries are especially recommended for patients with diabetes due to their low sugar intake.
All this has been demonstrated in human trials. The experiments were well coordinated, interpreted and translated.
Author Response
Thank you for your comments and for the review of our manuscript.
Reviewer 2 Report
The purpose of this study was to determine the impact consumption of berry anthocyanins and fiber on cellular respiration. An attempt to explain the impact of anthocyanins and fiber on the human body is very important and needed to consciously combat with obesity in our society. The manuscript is carefully written, modern analytical techniques were used to assess the content of anthocyanins in the juice and berries. The purpose of the research is clearly presented, and the need for research is justified in the introduction of the article.
The description of the analytical method for the determination of phenolic compounds, including anthocyanins, lacks information on the extraction of these compounds from freeze-dried berries. What extraction reagents were used?
Many anthocyanin compounds were identified in the work. In the description of the analytical methods, it was written that standards were used. Were all standards of anthocyanin compounds really held? If so, I recommend writing a producer of these standards.
Author Response
Thank you for your comments and for your review of our manuscript. We have made all the suggested changes. We added a clear description of our extraction protocol to the methods section. We also added a list of authentic standards with their sources. And we explained how we measured quantities of compounds for which there were no authentic standards.
Again, thank you for your review.
