The Skin Histopathology of Pro- and Parabiotics in a Mouse Model of Atopic Dermatitis
Abstract
:1. Introduction
2. Material and Methods
2.1. Strain and Atopic Agents
2.2. Animals
2.3. Blood Biochemistry Analysis
2.4. Measuring Skin Damage and Scratching Behavior
2.5. Histopathology and Microscopic Confirmation Using Skin Staining
2.6. Statistical Analysis
3. Results
3.1. Body Weight
3.2. Comparison of IgG and IgM Using Animal Serum
3.3. Anti-Atopic Effects of Pro- and Parabiotics Using H&E Staining
3.4. Anti-Atopic Effects of Pro- and Parabiotics via CD8 Antibody and Filaggrin Staining
3.5. Anti-Atopic Effects of Pro- and Parabiotics via Toluidine Blue Staining
3.6. Skin Damage and Scores by Date
4. Discussion
5. Conclusions
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Acknowledgments
Conflicts of Interest
References
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Day | |||
---|---|---|---|
1 | 8 | 15 | |
Vehicle Control group (VC) | 24.39 ± 0.61 NS | 24.70 ± 0.74 NS | 25.18 ± 0.73 NS |
Atopy-induced Negative Control group (NC) | 24.71 ± 1.82 NS | 25.06 ± 1.79 NS | 25.66 ± 1.31 NS |
Lactobacillus sakei T1 | 24.58 ± 1.35 NS | 24.95 ± 1.03 NS | 25.42 ± 1.40 NS |
Lactobacillus casei T2 | 24.00 ± 0.83 NS | 25.00 ± 1.43 NS | 25.65 ± 0.84 NS |
Lactobacillus sakei–Lactobacillus casei (4:3) T3 | 22.54 ± 1.30 NS | 23.22 ± 0.97 NS | 23.84 ± 0.87 NS |
IgG (mg/dL) | IgM (mg/dL) | |
---|---|---|
Vehicle Control group (VC) | 10.00 ± 2.70 A | 19.96 ± 2.74 A |
Atopy-induced Negative Control group (NC) | 18.12 ± 2.16 BC | 21.32 ± 2.32 AB |
Lactobacillus sakei T1 | 16.12 ± 1.36 B | 22.38 ± 3.40 AB |
Lactobacillus casei T2 | 19.30 ± 1.97 BC | 27.38 ± 5.64 B |
Lactobacillus sakei–Lactobacillus casei (4:3) T3 | 21.17 ± 3.84 C | 26.10 ± 0.79 AB |
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Kim, H.H.; Jeong, S.H.; Park, M.Y.; Bhosale, P.B.; Abusaliya, A.; Heo, J.D.; Kim, H.W.; Seong, J.K.; Kim, T.Y.; Park, J.W.; et al. The Skin Histopathology of Pro- and Parabiotics in a Mouse Model of Atopic Dermatitis. Nutrients 2024, 16, 2903. https://doi.org/10.3390/nu16172903
Kim HH, Jeong SH, Park MY, Bhosale PB, Abusaliya A, Heo JD, Kim HW, Seong JK, Kim TY, Park JW, et al. The Skin Histopathology of Pro- and Parabiotics in a Mouse Model of Atopic Dermatitis. Nutrients. 2024; 16(17):2903. https://doi.org/10.3390/nu16172903
Chicago/Turabian StyleKim, Hun Hwan, Se Hyo Jeong, Min Yeong Park, Pritam Bhagwan Bhosale, Abuyaseer Abusaliya, Jeong Doo Heo, Hyun Wook Kim, Je Kyung Seong, Tae Yang Kim, Jeong Woo Park, and et al. 2024. "The Skin Histopathology of Pro- and Parabiotics in a Mouse Model of Atopic Dermatitis" Nutrients 16, no. 17: 2903. https://doi.org/10.3390/nu16172903