Protective Effect of IgY Embedded in W/O/W Emulsion on LPS Enteritis-Induced Colonic Injury in Mice

Wang, Zhaohui; Ye, Ruihua; Xu, Zijian; Zhang, Shidi; Liu, Chuanming; Zhu, Kongdi; Wang, Pengjie; Huang, Jiaqiang

doi:10.3390/nu16193361

Open AccessArticle

Protective Effect of IgY Embedded in W/O/W Emulsion on LPS Enteritis-Induced Colonic Injury in Mice

by

Zhaohui Wang

¹,

Ruihua Ye

²,

Zijian Xu

¹,

Shidi Zhang

¹,

Chuanming Liu

¹,

Kongdi Zhu

¹,

Pengjie Wang

^3,*

and

Jiaqiang Huang

^1,*

¹

Key Laboratory of Precision Nutrition and Food Quality, Department of Nutrition and Health, Ministry of Education, China Agricultural University, Beijing 100083, China

²

College of Veterinary Medicine, China Agricultural University, Beijing 100083, China

³

Beijing Advanced Innovation Center for Food Nutrition and Human Health, China Agricultural University, Beijing 100083, China

^*

Authors to whom correspondence should be addressed.

Nutrients 2024, 16(19), 3361; https://doi.org/10.3390/nu16193361

Submission received: 30 August 2024 / Revised: 28 September 2024 / Accepted: 1 October 2024 / Published: 3 October 2024

(This article belongs to the Special Issue Inflammation and Nutritional Therapy)

Download

Browse Figures

Review Reports Versions Notes

Abstract

Chicken yolk immunoglobulin (IgY), an immunologically active component, is used as an alternative to antibiotics for the treatment of enteritis. In this study, IgY was embedded in a W/O/W emulsion to overcome the digestive barrier and to investigate the protective effect of IgY against LPS-induced enteritis in mice. Four different hydrophilic emulsifiers (T80, PC, SC, and WPI) were selected to prepare separate W/O/W emulsions for encapsulating IgY. The results showed that the IgY-embedded double emulsion in the WPI group was the most effective. IgY embedded in the W/O/W emulsion could reduce the damage of LPS to the mouse intestine and prevent LPS-induced intestinal mucosal damage in mice. It increased the number of cup cells, promoted the expression of Muc2, and increased the mRNA expression levels of KLF3, TFF3, Itln1, and Ang4 (p < 0.05). It also enhanced the antioxidant capacity of the colon tissue, reduced the level of inflammatory factors in the colon tissue, and protected the integrity of the colon tissue. Stable embedding of IgY could be achieved using the W/O/W emulsion. In addition, the IgY-embedded W/O/W emulsion can be used as a dietary supplement to protect against LPS-induced enteritis in mice.

Keywords: enteritis; IgY; LPS; W/O/W

Share and Cite

MDPI and ACS Style

Wang, Z.; Ye, R.; Xu, Z.; Zhang, S.; Liu, C.; Zhu, K.; Wang, P.; Huang, J. Protective Effect of IgY Embedded in W/O/W Emulsion on LPS Enteritis-Induced Colonic Injury in Mice. Nutrients 2024, 16, 3361. https://doi.org/10.3390/nu16193361

AMA Style

Wang Z, Ye R, Xu Z, Zhang S, Liu C, Zhu K, Wang P, Huang J. Protective Effect of IgY Embedded in W/O/W Emulsion on LPS Enteritis-Induced Colonic Injury in Mice. Nutrients. 2024; 16(19):3361. https://doi.org/10.3390/nu16193361

Chicago/Turabian Style

Wang, Zhaohui, Ruihua Ye, Zijian Xu, Shidi Zhang, Chuanming Liu, Kongdi Zhu, Pengjie Wang, and Jiaqiang Huang. 2024. "Protective Effect of IgY Embedded in W/O/W Emulsion on LPS Enteritis-Induced Colonic Injury in Mice" Nutrients 16, no. 19: 3361. https://doi.org/10.3390/nu16193361

APA Style

Wang, Z., Ye, R., Xu, Z., Zhang, S., Liu, C., Zhu, K., Wang, P., & Huang, J. (2024). Protective Effect of IgY Embedded in W/O/W Emulsion on LPS Enteritis-Induced Colonic Injury in Mice. Nutrients, 16(19), 3361. https://doi.org/10.3390/nu16193361

Note that from the first issue of 2016, this journal uses article numbers instead of page numbers. See further details here.

Article Menu

Protective Effect of IgY Embedded in W/O/W Emulsion on LPS Enteritis-Induced Colonic Injury in Mice

Abstract

Share and Cite

Article Metrics

Article Access Statistics

Further Information

Guidelines

MDPI Initiatives

Follow MDPI